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Screening of lipases from mangrove sediments by metagenomic and cultivation
Araújo, Francisco Jonathan dos Santos; Santiago, Saulo Gonçalves; Nogueira, Vanessa Lúcia Rodrigues; Pereira, Mirella Leite; Hissa, Denise CavalcanteMangroves are costal ecosystems where extreme environmental conditions are found, being considered valuable source for the discovery of novel microbial enzymes. Lipases constitute the most important group of biocatalysts being used in various biotechnological applications. In this context, the current work aimed to screening bacterial isolates and metagenomic clones from mangrove sediments for lipase/esterase p...
Avaliação de proteases e biossurfactantes produzidos por Bacillus subtilis TIM2...
Araújo, Francisco Jonathan dos SantosThe growing demand of modern society for ecofriendly cleaning products has stimulated the search of natural additives and biodegradable products. Since petroleum-derived surfactants added to commercial enzymes are the key components of the major cleaning products, the replacement of synthetic these surfactants by biosurfactants supplemented with microbial enzymes is an alternative for the new generation cleanin...
Expression and purification of a new esterase from metagenomic clone similar to...
Araújo, Francisco Jonathan dos Santos; Gomes, Brenda Suellen Rodrigues; Bessa, Claudiane Carvalho; Soares Júnior, João Alberto de OliveiraEsterases are lipolytic enzymes widely used in industrial applications. This work aimed the overexpression and purification of the esterase LipG7 identified from a metagenomic library constructed from mangrove sediments, which gene sequence shares 80% amino acid identity to 1,4-butanediol diacrylate esterase from the bacterium Porticoccus hydrocarbonoclasticus. LipG7 was expressed in three commercial Escherichi...