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Refinement of two-dimensional electrophoresis for vitreous proteome profiling u...
Santos, Fátima Raquel Milhano dos; Albuquerque, Tânia Gonçalves; Gaspar, Leonor Isabel Mesquita; Dias, Joao ML; Sousa, João Paulo Castro DeDespite technological advances, two-dimensional electrophoresis (2DE) of biological fluids, such as vitreous, remains a major challenge. In this study, artificial neural network was applied to optimize the recovery of vitreous proteins and its detection by 2DE analysis through the combination of several solubilizing agents (CHAPS, Genapol, DTT, IPG buffer), temperature, and total voltage. The highest protein re...
Data: 2019
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Origem: uBibliorum