8 documents found, page 1 of 1
Polyhydroxyalkanoates from industrial cheese whey: production and characterizat...
Carvalheira, Mónica; Hilliou, Loic; Oliveira, Catarina S. S.; Guarda, Eliana C.; Reis, Maria A. M.The composition of polyhydroxyalkanoate (PHA) monomers affects the properties and final applications of PHA polymers. This study focused on the feasibility of producing tailored PHA differing in hydroxyvalerate (HV) content, through manipulation of the acidogenic fermented stream composition, and on the characterization of PHA properties to determine the best composition for melt processing. Cheese whey was use...
Valorization of brewery waste through polyhydroxyalkanoates production supporte...
Carvalheira, Mónica; Amorim, Catarina L.; Oliveira, Ana Catarina; Guarda, Eliana C.; Costa, Eunice; Teixeira, Margarida Ribau; Castro, Paula M. L.Raw brewers’ spent grain (BSG), a by-product of beer production and produced at a large scale, presents a composition that has been shown to have potential as feedstock for several biological processes, such as polyhydroxyalkanoates (PHAs) production. Although the high interest in the PHA production from waste, the bioconversion of BSG into PHA using microbial mixed cultures (MMC) has not yet been explored. Thi...
Polyhydroxyalkanoates from industrial cheese whey: production and characterizat...
Carvalheira, Mónica; Hilliou, L.; Oliveira, Catarina S. S.; Guarda, Eliana C.; Reis, Maria A. M.The composition of polyhydroxyalkanoate (PHA) monomers affects the properties and final applications of PHA polymers. This study focused on the feasibility of producing tailored PHA differing in hydroxyvalerate (HV) content, through manipulation of the acidogenic fermented stream composition, and on the characterization of PHA properties to determine the best composition for melt processing. Cheese whey was use...
Valorization of brewery waste through Polyhydroxyalkanoates production supporte...
Carvalheira, Mónica; Amorim, Catarina L.; Oliveira, Ana Catarina; Guarda, Eliana C.; Costa, Eunice; Ribau Teixeira, Margarida; Castro, Paula M. L.Raw brewers’ spent grain (BSG), a by-product of beer production and produced at a large scale, presents a composition that has been shown to have potential as feedstock for several biological processes, such as polyhydroxyalkanoates (PHAs) production. Although the high interest in the PHA production from waste, the bioconversion of BSG into PHA using microbial mixed cultures (MMC) has not yet been explored. Thi...
Monitoring intracellular polyphosphate accumulation in enhanced biological phos...
Mesquita, D. P.; Amaral, A. L.; Leal, C.; Carvalheira, Mónica; Cunha, J. R.; Oehmen, Adrian; Reis, Maria A. M.; Ferreira, Eugénio C.A rapid methodology for intracellular storage polyphosphate (poly-P) identification and monitoring in enhanced biological phosphorus removal (EBPR) systems is proposed based on quantitative image analysis (QIA). In EBPR systems, 4′,6-diamidino-2-phenylindole (DAPI) is usually combined with fluorescence in situ hybridization to evaluate the microbial community. The proposed monitoring technique is based on a QIA...
Biopolymer monitoring using quantitative image analysis techniques
Carvalheira, Mónica; Mesquita, D. P.; Leal, Cristiano; Oehmen, Adrian; Carvalho, Gilda; Cunha, J. R.; Amaral, A. L.; Ferreira, Eugénio C.Polyhydroxyalkanoates (PHAs) are intracellular biopolymers with many applications, particularly as substitutes of polypropylene and polyethylene, due to their thermoplastic properties and biocompatible nature. Furthermore, glycogen is a polysaccharide of glucose with high importance in the metabolism of microbial communities and polyphosphate is a microbial storage compound that should be recovered in order to ...
Intracellular poly-P assessment by DAPI staining and image analysis
Amaral, A. L.; Mesquita, D. P.; Leal, C.; Carvalheira, Mónica; Oehmen, Adrian; Reis, Maria A. M.; Ferreira, Eugénio C.In wastewater treatment, enhanced biological phosphorus removal (EBPR) is considered a well-established process to remove phosphate (P). EBPR is based on the activity of polyphosphate-accumulating organisms (PAOs) able to take up and store large amounts of P as intracellular (poly-P) granules. However, monitoring poly-P in mixed cultures is usually performed by a laborious and time consuming off-line chemical a...
Novel method to quantify intracellular accumulation of polyphosphate in EBPR sy...
Mesquita, D. P.; Amaral, A. L.; Leal, Cristiano; Carvalheira, Mónica; Cunha, J. R.; Oehmen, Adrian; Reis, Maria A. M.; Ferreira, Eugénio C.A new method for intracellular storage polyphosphate (poly-P)identification and quantification in enhanced biological phosphorus removal (EBPR) systems is proposed based on image analysis. In EBPR systems, 4',6-diamidino-2-phenylindole (DAPI) is usually combined with fluororescent in situ hybridization (FISH) to evaluate the microbial community. The proposed technique is based on an image analysis procedure spe...