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Simultaneous Detection of Mycobacterium tuberculosis and Mycobacterium avium Co...

Abstract Infection disease caused by nontuberculous mycobacteria (NTM) have been increasingly reported and often manifest with the same symptoms of tuberculosis (TB). The identification of the causative agent is fundamental for the determination of an appropriate therapy, since each species of mycobacteria requires a specific treatment. Thus, rapid and accurate tests to identify mycobacteria of medical interest...

Green propolis as an adjuvant against nontuberculous mycobacteria

Abstract Natural products have been touted as important tools because of their vast potential for the development of compounds with antimicrobial activity and the possible inhibitory activity and/or adjuvant resistance mechanisms. Propolis has been empirically used for many years for the treatment of diseases, mainly due to its antioxidant, anti inflammatory and antimicrobial activities. This study aimed to eva...

Duroia saccifera: in vitro germination, friable calli and identification of β-s...

Abstract Duroia saccifera (Rubiaceae) occurs in the Amazon rainforest and their extracts showed antibacterial properties. To obtain greater quantities of active substances, leaf segments from in vitro D. saccifera seedlings were used as explants for calli induction; calli were multiplied via multiple subcultures, dried and extracted with hexane followed by ethyl acetate (EtOAc) and methanol (MeOH). As D. macrop...

Nontuberculous mycobacteria in patients with suspected tuberculosis and the gen...

ABSTRACT Objective: Nontuberculous mycobacteria (NTM) are a heterogeneous group of bacteria that are widely distributed in nature and associated with opportunistic infections in humans. The aims of this study were to identify NTM in patients with suspected tuberculosis who presented positive cultures and to evaluate the genetic diversity of strains identified as Mycobacterium avium. Methods: We studied pulmonar...

O processo de detecção e tratamento de casos de tuberculose em um presídio

Resumo O estudo objetiva analisar o processo de detecção e tratamento de casos de tuberculose (TB) em um presídio do sul do Brasil. Realizou-se busca ativa e passiva para estimar a magnitude da TB entre uma população de 764 detentos. Simultaneamente às estratégias de detecção e ao seguimento clínico dos 41 casos confirmados, realizou-se observação participante e registros em diário de campo, o que possibilitou ...

Tuberculose em presídios brasileiros: uma revisão integrativa da literatura

Resumo Tuberculosis in Brazilian prisons: an integrative review of the literature O objetivo deste estudo foi sistematizar o conhecimento produzido sobre tuberculose em presídios brasileiros. Foi realizada uma revisão integrativa nas bases Medline, Lilacs e biblioteca eletrônica SciELO, com os descritores: “Tuberculosis”, “Prisons” e “Brazil”. Entre os 61 registros encontrados, 33 foram avaliados (28 duplicados...

Antimycobacterial and cytotoxicity activity of microcystins

Background The present work aimed to evaluate the antimycobacterial activity and cytotoxicity of Microcystis aeruginosa toxins, the MC-LR variant and purified extract of [D-Leu1] microcystin-LR. Methods The antimicrobial activity of M. aeruginosa extract and microcystin was evaluated by resazurin microtiter assay against Mycobacterium tuberculosis, M. terrae, M. chelonae and M. kansasii. The cytotoxicity assay ...

Molecular typing of Mycobacterium bovis isolated in the south of Brazil

Bovine tuberculosis is a major infectious disease of the cattle. In this study, 85 M. bovis isolates from 162 lymph nodes, obtained from a herd of cattle on a farm in southern Brazil, were evaluated using spoligotyping and VNTR. The strains were grouped into five clusters and five orphans, showing a heterogenic genetic profile, what could represent diverse geographic origins of the introduced cows and/or the fr...

Molecular typing of Mycobacterium bovis isolates: a review

Mycobacterium bovis is the main causative agent of animal tuberculosis (TB) and it may cause TB in humans. Molecular typing of M. bovis isolates provides precise epidemiological data on issues of inter- or intra-herd transmission and wildlife reservoirs. Techniques used for typing M. bovis have evolved over the last 2 decades, and PCR-based methods such as spoligotyping and mycobacterial interspersed repetitive...

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