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Smoking Induces a Decline in Semen Quality and the Activation of Stress Respons...
Henriques, Magda Carvalho; Santiago, Joana; Patrício, António; Herdeiro, Maria Teresa; Loureiro, Susana; Fardilha, MargaridaMale infertility is a prevalent concern affecting couples worldwide. While genetic factors, hormonal imbalances, and reproductive system defects play significant roles, emerging evidence suggests that lifestyle choices also profoundly impact male fertility. This study aimed to explore the effects of several lifestyle factors, including tobacco and alcohol consumption, physical activity, and dietary habits, on s...
The World Café method and spaces dedicated to active teaching & learning: A dyn...
Pinto-Pinho, Patrícia; Ferreira, Ana; Matos, Bárbara; Santiago, Joana; Henriques, Magda Carvalho; Corda, Pedro Oliveira; Lima, Tânia; Rodrigues, MárioA pilot experiment was developed in a Biomedical Sciences curricular unit to test how using a different knowledge-sharing method (World Café) in a Space for Active Learning and Teaching would affect students’ engagement. Each working group was asked to prepare a poster summarising a different topic about the physiology of the oocyte. On the activity day, rotating discussion sessions moderated by tutors took pla...
Organoids of the male reproductive system: Challenges, opportunities, and their...
Patrício, Daniela; Santiago, Joana; Mano, João F; Fardilha, MargaridaOrganoids are units of function of a given organ able to reproduce, in culture, a biological structure similar in architecture and function to its counterpart in vivo. Today, it is possible to develop an organoid from a fragment of tissue, a stem cell located in an adult organ, an embryonic stem cell, or an induced pluripotent stem cell. In the past decade, many organoids have been developed which mimic stomach...
Age-Dependent Alterations in Semen Parameters and Human Sperm MicroRNA Profile
Santiago, Joana; Silva, Joana V.; Santos, Manuel A. S.; Fardilha, MargaridaThe trend to delay parenthood is increasing, impacting fertility and reproductive outcomes. Advanced paternal age (APA), defined as men's age above 40 years at conception, has been linked with testicular impairment, abnormal semen parameters, and poor reproductive and birth outcomes. Recently, the significance of sperm microRNA for fertilization and embryonic development has emerged. This work aimed to investig...
Effects of Age and Lifelong Moderate-Intensity Exercise Training on Rats' Testi...
Silva, Joana V.; Santiago, Joana; Matos, Bárbara; Henriques, Magda C.; Patrício, Daniela; Martins, Ana D.; Duarte, José A.; Ferreira, RitaAging is associated with testicular morphological and functional alterations, but the underlying molecular mechanisms and the impact of physical exercise are poorly understood. In this study, we examined the effects of age and lifelong moderate-intensity exercise on rat testis. Mature adults (35 weeks) and middle-aged (61 weeks) Wistar Unilever male rats were maintained as sedentary or subjected to a lifelong m...
G-Protein Coupled Receptors in Human Sperm: An In Silico Approach to Identify P...
Corda, Pedro O.; Santiago, Joana; Fardilha, MargaridaG protein-coupled receptors (GPCRs) are involved in several physiological processes, and they represent the largest family of drug targets to date. However, the presence and function of these receptors are poorly described in human spermatozoa. Here, we aimed to identify and characterize the GPCRs present in human spermatozoa and perform an in silico analysis to understand their potential role in sperm function...
PP1, PP2A and PP2B Interplay in the Regulation of Sperm Motility: Lessons from ...
Ferreira, Ana F.; Santiago, Joana; Silva, Joana V.; Oliveira, Pedro F.; Fardilha, MargaridaMale fertility relies on the ability of spermatozoa to fertilize the egg in the female reproductive tract (FRT). Spermatozoa acquire activated motility during epididymal maturation; however, to be capable of fertilization, they must achieve hyperactivated motility in the FRT. Extensive research found that three protein phosphatases (PPs) are crucial to sperm motility regulation, the sperm-specific protein phosp...
New evidences of ubiquitin-proteasome system activity in human sperm
Silva, Joana Vieira; Santiago, Joana; Sousa, Mário; Oliveira, Pedro F; Fardilha, MargaridaSem resumo disponível.
Fighting Bisphenol A-Induced Male Infertility: The Power of Antioxidants
Santiago, Joana; Silva, Joana V.; Santos, Manuel; Fardilha, MargaridaBisphenol A (BPA), a well-known endocrine disruptor present in epoxy resins and polycarbonate plastics, negatively disturbs the male reproductive system affecting male fertility. In vivo studies showed that BPA exposure has deleterious effects on spermatogenesis by disturbing the hypothalamic-pituitary-gonadal axis and inducing oxidative stress in testis. This compound seems to disrupt hormone signalling even a...
Bovine semen sexing: Sperm membrane proteomics as candidates for immunological ...
Quelhas, Joana; Santiago, Joana; Matos, Bárbara; Rocha, António; Lopes, Graça; Fardilha, MargaridaThe use of sexed semen in dairy and beef farms ensures the production of animals of the desired sex, resulting in a reduction of costs and an improvement of environmental sustainability. Several methods have been developed over the years, but most of them were abandoned due to their limited efficacy. Currently, the only commercially available method for the separation of X- and Y-chromosome-bearing sperm is flu...