23 documents found, page 1 of 3
Natural infection in ovarian structures by bovine herpesvirus 1: molecular and ...
Pereira, Emílio César Martins; Costa, Eduardo Paulino da; Silva Júnior, Abelardo; Mendes, Vívian Rachel de Araújo; Santos, Giancarlo Magalhães dosNeste trabalho foi avaliada a presença do DNA viral, por meio da Reação em Cadeia da Polimerase (PCR), no tecido ovariano, nos oócitos, líquido folicular e sangue de vacas naturalmente infectadas. Também foi avaliado o perfil sorológico dos animais amostrados. Foram coletadas amostras de soro, sangue, tecido ovariano, líquido folicular e complexo cumulus-oócitos de 147 animais abatidos em frigorífico não vacina...
Cytokine gene expression and molecular detection of Mycobacterium avium subspec...
Schwarz, David G. G.; Pietralonga, Pricila A. G.; Souza, Marina C. C.; Carvalho, Isabel A.; Cruzeiro, Rosyane S.; Malaquias, Juaci V.Mycobacterium avium subespécie paratuberculosis (MAP) pode infectar ruminantes e permanecer subclínica por longos períodos nos rebanhos. A identificação de órgãos mais susceptíveis à infecção e a avaliação da expressão das citocinas no local da infecção são importantes para compreender a patogênese de MAP. Neste estudo foi avaliada a probabilidade de detecção de DNA de MAP e a expressão de citocinas em órgãos d...
Detection of bovine herpesvirus 1 in cumulus-oocyte complexes of cows
Queiroz-Castro, Vanessa L. D.; Costa, Eduardo P. da; Alves, Saullo Vinicius P.; Silva Júnior, Abelardo; Machado-Neves, Mariana; Guimarães, José D.Bovine herpesvirus 1 (BoHV-1) is the causative agent of infectious bovine rhinotracheitis (IBR) and is also associated with reproductive failure. This study investigated the presence of BoHV-1 in cumulus-oocyte complexes (COCs) of naturally-infected cows without clinical signs of IBR. The presence of BoHV-1 in COCs was evaluated by immunofluorescence using confocal laser scanning microscopy. Blood samples and o...
Imunogenicidade de isolados de herpesvírus bovino 5 como candidatos à vacina
Souza, Luiz Felipe Lourenço de; Silva Júnior, Abelardo; Freitas, Carla Maria Batista de; Flores, Eduardo Furtado; Almeida, Márcia Rogéria deO herpesvírus bovino 5 (BoHV-5) é o agente da menigoencefalite herpética bovina. A doença neurológica, associada à infecção pelo BoHV-5, apresenta altas taxas de letalidade em bovinos jovens e está disseminada no Brasil. A prevenção das perdas causadas pela infecção pelo herpesvírus está baseada, principalmente, na imunização dos animais. Nesse sentido, foi delineada uma comparação entre isolados de BoHV-5, bus...
Antimetastatic effect of the pharmacological inhibition of serine/arginine-rich...
Moreira, Gabriela Alves; Lima, Graziela Domingues de Almeida; Siqueira, Raoni Pais; Barros, Marcus Vinícius de AndradeThe Serine/arginine-rich protein kinases (SRPK) are involved in pre-mRNA splicing control through the phosphorylation of the SR protein family of splicing factors. Over the last years, several studies have shown the relevance of SRPK for human cancers and their potential as promising drug targets. In this context, we have previously selected three trifluoromethyl arylamides (named here as SRVIC24, SRVIC30 and S...
Trifluoromethyl arylamides with antileukemia effect and intracellular inhibitor...
Siqueira, Raoni Pais; Barros, Marcus Vinícius de Andrade; Barbosa, Éverton de Almeida Alves; Onofre, Thiago Souza; Gonçalves, Victor Hugo SousaThe serine/arginine-rich protein kinases (SRPKs) have frequently been found with altered activity in a number of cancers, suggesting they could serve as potential therapeutic targets in oncology. Here we describe the synthesis of a series of twenty-two trifluoromethyl arylamides based on the known SRPKs inhibitor N-(2-(piperidin-1-yl)-5-(trifluoromethyl)phenyl)isonicotinamide (SRPIN340) and the evaluation of th...
A porcine circovirus-2 mutant isolated in Brazil contains low-frequency substit...
Salgado, Rafael Locatelli; Vidigal, Pedro Marcus Pereira; Gonzaga, Natalia F.; Souza, Luiz F. L. de; Polêto, Marcelo D.; Onofre, Thiago SouzaPorcine circovirus-2 (PCV2) is the etiologic agent of several diseases in pigs, including multi-systemic wasting syndrome (PMWS). In this work, a new mutant PCV2b was isolated from PMWS-affected pigs on a Brazilian farm. Its genome showed high sequence similarity (>99 % identity) to those from a group of emerging mutants isolated from cases of PMWS outbreaks in vaccinated pigs in China, the USA and South Korea....
Distribution of infectious bronchitis virus strains in different organs and evi...
Pereira, Claiton Gonçalves; Saraiva, Giuliana Loreto; Vidigal, Pedro Marcus Pereira; Fietto, Juliana Lopes Rangel; Bressan, Gustavo CostaOn the basis of partial sequencing of the infectious bronchitis virus (IBV) S1 gene, this study investigated the molecular diversity of the virus in two life periods of a batch of breeding hens at the field level. The chicks were vaccinated against IBV on the second day of life with the vaccine Ma5, but at the age of 18 days, they exhibited clinical signs and macroscopic lesions compatible with avian infectious...
Antibodies against Bovine herpesvirus 1 in dairy herds in the state of Espirito...
Santos, Marcus Rebouças; Ferreira, Hanna Carolina Campos; Santos, Marcos Antônio dos; Saraiva, Giuliana Loreto; Tafuri, Natália FilardiO herpesvírus bovino 1 (Bovine herpesvirus 1 - BoHV-1) causa grandes prejuízos econômicos à pecuária bovina mundial, por comprometer os sistemas respiratório e reprodutivo de bovinos de corte e leiteiros. Nas últimas décadas, os registros de casos clínicos ocasionados pelo BoHV-1, no Brasil, mostram que têm aumentado gradualmente, o que torna importante o delineamento de estudos que mostrem a distribuição da in...
Inhibition of herpes simplex virus 1 (HSV-1) and poliovirus (PV-1) by bacterioc...
Cavicchioli, Valéria Quintana; Carvalho, Otávio Valério de; Paiva, Janine Cerqueira de; Todorov, Svetoslav Dimitrov; Silva Júnior, AbelardoBacteriocins have unusual inhibitory activity, including antiviral properties, and this can be exploited to give alternative applications. Semi–purified bacteriocins of six lactic acid bacteria (LAB) strains isolated from goat milk (two Lactococcus lactis: GLc03 and GLc05, and four Enterococcus durans: GEn09, GEn12, GEn14 and GEn17) were tested for cytotoxicity in Vero cells (50% Cytotoxicity Concentration: CC5...