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KSP and MPS1 kinases as potential therapeutic targets for ovarian cancer
Sampaio, Francisca; Gomes, Carina; Silva, João P. N.; Bousbaa, Hassan; Silva, Patrícia M. A.Background: Ovarian cancer ranks among the top causes of cancer-related deaths in women worldwide [1]. The conventional treatment for ovarian cancer involves surgery and chemotherapy, typically using a combination of paclitaxel and carboplatin [2]. However, despite the initial positive response to this treatment regimen, the development of treatment resistance has emerged as a significant challenge in managing ...
KSP and Aurora B as potential biomarkers and therapeutic targets for head and n...
Silva, João P. N.; Silva, Raquel; Vilas-Boas, Maria Do Carmo; Silva, Patrícia M. A. Silva; Bousbaa, HassanBackground: Head and neck cancer (HNC) is the seventh most prevalent worldwide [1]. The treatment for HNC often involves a combination of modalities such as surgery, radiation therapy, and chemotherapy. However, HNC patients’ survival rate has shown little improvement [2]. Treatment failures are often attributed to local recurrence, lymph node metastases, and drug resistance [3]. Hence, the need for new biomark...
Co-inhibition of mitotic kinesins and kinases with BCL-2 family inhibitors enha...
Silva, João P. N.; Monteiro, L.; Silva, Patrícia M.A.; Bousbaa, HassanBackground: Head and neck cancer is the seventh most prevalent cancer worldwide and it includes oral cancer [1]. Approximately 90% of all oral cancers are oral squamous cell carcinomas [2,3]. Despite their promising preclinical results, the inhibitors of mitotic kinesins and kinases failed in clinical trials, probably because they are not too effective in inducing apoptosis when used in monotherapy [4,5]. Thus,...
Effective cytocompatible nanovectors based on serine-derived gemini surfactants...
Costa, Catarina; Oliveira, Isabel S.; Silva, João P. N.; Silva, Sandra G.; Botelho, Cláudia M.; Vale, M. C.; Real Oliveira, M. Elisabete C.D.Non-viral gene therapy based on gene silencing with small interfering RNA (siRNA) has attracted great interest over recent years. Among various types of cationic complexation agents, amino acid-based surfactants have been recently explored for nucleic acid delivery due to their low toxicity and high biocompatibility. Monoolein (MO), in turn, has been used as helper lipid in liposomal systems due to its ability ...
Tunable pDNA/DODAB:MO lipoplexes: the effect of incubation temperature on pDNA/...
Silva, João P. N.; Oliveira, Ana Cristina Norberto Gonçalves; Lúcio, M.; Gomes, Andreia C; Coutinho, Paulo J. P.; Oliveira, M. ElisabetetDioctadecyldimethylammonium bromide (DODAB):1-monooleoyl-rac-glycerol (MO) cationic liposomeswere reported as a promising alternative to common transfection agents, showing superior effective-ness on the transfection of the 293T mammalian cell line with pSV- -gal plasmid DNA. The study ofDODAB:MO aggregates in the absence of DNA has indicated that their morphology depends on the balancebetween DODAB’s tendency...
Structural dynamics and physicochemical properties of pDNA/DODAB:MO lipoplexes ...
Silva, João P. N.; Oliveira, I. M. S. C.; Oliveira, Ana Cristina Norberto Gonçalves; Lúcio, M.; Gomes, Andreia C; Coutinho, Paulo J. G.Dioctadecyldimethylammoniumbromide (DODAB):Monoolein (MO) lipoplexes have mainly been studied within the range of high molar ratios of DODAB, with noticeable transfection efficiencies in the Human Embryonic Kidney (HEK, a.k.a. 293T) cell line. In thiswork,we intend to study the effect of highMOcontent on the structure and physicochemical properties of pDNA/DODAB:MOlipoplexes to achieve some correlationwith thei...
Development of dioctadecyldimethylammonium bromide/monoolein liposomes for gene...
Silva, João P. N.; Oliveira, Ana Cristina Norberto Gonçalves; Gomes, A. C.; Oliveira, M. ElisabeteThe artificial introduction of nucleic acids (NA) into mammalian cells (transfection) has become, in recent years, a well-established procedure in basic and applied research, which allowed the study of gene function and regulation. The advances in this area have made possible the use of these methods for gene-based medicines, which constitute alternative therapeutic approaches. One of the most prominent methods...
Aggregation behavior of aqueous dioctadecyldimethylammonium bromide/monoolein m...
Oliveira, I. M. S. C.; Silva, João P. N.; Feitosa, Eloi; Marques, E. F.; Castanheira, Elisabete M. S.; Oliveira, M. ElisabeteA recently described non-viral gene delivery system [dioctadecyldimethylammonium bromide (DODAB)/ monoolein (MO)] has been studied in detail to improve knowledge on the interactions between lamellar (DODAB) and non-lamellar-forming (MO) lipids, as a means to enhance their final cell transfection efficiency. Indeed, the morphology, fluidity, and size of these cationic surfactant/neutral lipid mixtures play an im...