9 documents found, page 1 of 1
Body image concerns in people who underwent a total laryngectomy
Guimarães, Isabel; Torrejano, Gabriela; Aires, Raquel; Gonçalves, Filomena; Freitas, Susana Vaz; Correia, Paula; Romeiro, Cláudia; Silvestre, InêsAbstract: Background: Body image is a potential psychological burden after total laryngectomy (TL) with devastating effects on patients’ health-related quality of life (HRQOL) and communication. This study focused on TL patients to determine the prevalence of dissatisfied body image and whether they have poorer HRQOL and difficulty adjusting to their new voice than TL patients with satisfied body image. It also...
O Centro de Arte Contemporânea e o Museu Nacional de Soares dos Reis. Análise d...
Silvestre, InêsO Centro de Arte Contemporânea (CAC) funcionou entre 1976 e 1980 no Museu Nacional de Soares dos Reis (MNSR), no Porto, com vista à exposição, estudo e divulgação de arte contemporânea. Este artigo tem como objetivo analisar a parceria institucional estabelecida entre o CAC e o MNSR durante esses quatro anos. Para tal, torna-se importante contextualizar a criação do CAC a partir da perspetiva da ausência de um ...
Biofilm Formation by ST17 and ST19 Strains of Streptococcus agalactiae
Silvestre, Inês; Borrego, Maria José; Jordão, LuísaBacterial biofilms are an important virulence factor with a vital role in evasion from the host immune system, colonization and infection. The aim of the present study was to evaluate in vitro the effects of three environmental factors (H+ , glucose and human plasma) in biofilm formation, by carrier and invasive S. agalactiae strains of ST17 and ST19 sequence types, including DNase producers and nonproducers. B...
Global gene expression analysis of Streptococcus agalactiae at exponential grow...
Silvestre, Inês; Borges, Vítor; Duarte, Sílvia; Nunes, Alexandra; Sobral, Rita; Vieira, Luís; Gomes, João Paulo; Borrego, Maria JoséA comparative overview of the global gene expression levels of S. agalactiae reference strain NEM316 at the exponential growth phase was done through RNA-sequencing. The expression levels of 47 genes potentially linked to virulence evidenced that: i) the major nuclease, GBS_RS03720/gbs0661, presented higher mean expression values than the remainder of DNase genes; ii) the genetic pilus island PI-2a genes presen...
Genomic insights on DNase production in Streptococcus agalactiae ST17 and ST19 ...
Silvestre, Inês; Nunes, Alexandra; Borges, Vítor; Isidro, Joana; Silva, Catarina; Vieira, Luís; Gomes, João Paulo; Borrego, Maria JoséStreptococcus agalactiae evasion from the human defense mechanisms has been linked to the production of DNases. These were proposed to contribute to the hypervirulence of S. agalactiae ST17/capsular-type III strains, mostly associated with neonatal meningitis. We performed a comparative genomic analysis between ST17 and ST19 human strains with different cell tropism and distinct DNase production phenotypes. All...
Biofilm formation by ST17 and ST19 strains of Streptococcus agalactiae
Silvestre, Inês; Borrego, Maria José; Jordão, LuísaBacterial biofilms are an important virulence factor with a vital role in evasion from the host immune system, colonization and infection. The aim of the present study was to evaluate in vitro the effects of three environmental factors (H+, glucose and human plasma) in biofilm formation, by carrier and invasive Streptococcus agalactiae strains of ST17 and ST19 sequence types, including DNase producers and non-p...
Self-evaluation of communication experiences after laryngectomy (SECEL): transl...
Guimarães, Isabel; Torrejano, Gabriela; Aires, Raquel; Freitas, Susana; Gonçalves, Filomena; Correia, Paula; Romeiro, Cláudia; Silvestre, InêsIntroduction: The patients’ perception of how communication dysfunction may cause a disadvantage is important information for the clinical decision-making process. Objectives: This study aimed to translate the self-evaluation of communication experiences after laryngectomy (SECEL) to the European Portuguese (EP) and to assess its feasibility, acceptability, reliability, and validity. Material and methods: A cro...
Capsular Type, Sequence Type and Microbial Resistance Factors Impact on DNase A...
Florindo, Carlos; Barroco, Cinthia Alves; Silvestre, Inês; Damião, Vera; Gomes, João Paulo; Spellerberg, Barbara; Santos-Sanches, IldaExtracellular deoxyribonucleases (DNases) contribute to the spread of pathogenic bacteria through the evasion from host innate immunity. Our main objective was to evaluate the production of extracellular DNases by human and bovine Streptococcus agalactiae clinical strains and perform a correlation of genetic lineages and DNase activity with capsular type, genetic determinants, clinical origin (colonization and ...
Voice quality on women with vocal nodules diagnosis: Preliminary study
Silvestre, Inês; Guimarães, Isabel; Teixeira, AntónioObjectives: (1) identify the degree and type of voice disorder; (2) to determine the acoustic parameters values; (3) to assess the relationship between perceptual evaluation and the different voice parameters.Methods: 15 adult women, with vocal nodules diagnosis, performed a vocal perceptual evaluation through the use of GRBAS scale and an acoustic analysis, through the software program Praat, of /a/, /i / and ...