2 documents found, page 1 of 1
Density separation of petrous bone powders for optimized ancient DNA yields
Fernandes, Daniel; Sirak, Kendra A.; Cheronet, Olivia; Novak, Mario; Brück, Florian; Zelger, Evelyn; Llanos-Lizcano, Alejandro; Wagner, AnnaDensity separation is a process routinely used to segregate minerals, organic matter, and even microplastics, from soils and sediments. Here we apply density separation to archaeological bone powders before DNA extraction to increase endogenous DNA recovery relative to a standard control extraction of the same powders. Using nontoxic heavy liquid solutions, we separated powders from the petrous bones of 10 indi...
Ancient DNA and deep population structure in sub-Saharan African foragers
Lipson, Mark; Sawchuk, Elizabeth A.; Thompson, Jessica C.; Oppenheimer, Jonas; Tryon, Christian A.; Ranhorn, Kathryn L.; de Luna, Kathryn M.Multiple lines of genetic and archaeological evidence suggest that there were major demographic changes in the terminal Late Pleistocene epoch and early Holocene epoch of sub-Saharan Africa(1-4). Inferences about this period are challenging to make because demographic shifts in the past 5,000 years have obscured the structures of more ancient populations(3,5). Here we present genome-wide ancient DNA data for si...