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An in vitro fermentation model to study the impact of bacteriophages targeting ...

Pinto, Graça; Shetty, Sudarshan A.; Zoetendal, Erwin G.; Gonçalves, Raquel F. S.; Pinheiro, Ana Cristina; Almeida, Carina Manuela Fernandes

Lytic bacteriophages are considered safe for human consumption as biocontrol agents against foodborne pathogens, in particular in ready-to-eat foodstuffs. Phages could, however, evolve to infect different hosts when passing through the gastrointestinal tract (GIT). This underlines the importance of understanding the impact of phages towards colonic microbiota, particularly towards bacterial families usually fou...


Unveiling the impact of STEC infecting phages on the colon microbiota using an ...

Pinto, Graça; Shetty, Sudarshan; Zoetendal, Erwin G.; Gonçalves, Raquel Filipa Silva; Pinheiro, Ana Cristina; Almeida, Carina Manuela Fernandes

(Bacterio)phages are considered safe for humans consumption, being regard as excellent biocontrol tools to prevent foodborne pathogens spread. Phages major advantage is their inherent specificity towards a bacterial species, yet some reports have shown phages ability to evolve to infect different hosts when transiting the gastrointestinal tract (GIT). And so, it is of extreme importance to understand the safety...


Safety assessment of the biocontrol of Shiga toxin-producing Escherichia coli u...

Pinto, Maria Graça Cerqueira; Minnich, Scott A.; Hovde, Carolyn J.; Oliveira, Hugo Alexandre Mendes; Smidt, Hauke; Almeida, Carina Manuela Fernandes

Shiga-toxin producing Escherichia coli (STEC) is an important foodborne pathogen. Although strictly lytic phages have been considered promising tools for STEC biocontrol, safety issues related to Shiga toxin production and emergence of bacteriophage insensitive mutants (BIMs) need to be investigated. We report the isolation of two lytic phages, vB_EcoS_Ace (Ace) and vB_EcoM_Shy (Shy) and the characterization of...


Proteogenomic analysis of Georgfuchsia toluolica revealed unexpected concurrent...

Atashgahi, Siavash; Oosterkamp, Margreet J.; Peng, Peng; Frank, Jeroen; Kraft, Beate; Hornung, Bastian; Schleheck, David; Lücker, Sebastian

Denitrifying Betaproteobacteria play a key role in the anaerobic degradation of monoaromatic hydrocarbons. We performed a multi-omics study to better understand the metabolism of the representative organism Georgfuchsia toluolica strain G5G6 known as a strict anaerobe coupling toluene oxidation with dissimilatory nitrate and Fe(III) reduction. Despite the genomic potential for degradation of different carbon so...


The interactions of bacteriophage Ace and Shiga toxin-producing Escherichia col...

Pinto, Graça; Minnich, Scott A.; Hovde, Carolyn J.; Oliveira, Hugo Alexandre Mendes; Smidt, Hauke; Almeida, Carina Manuela Fernandes; Azeredo, Joana

Strictly lytic phages are considered powerful tools for biocontrol of foodborne pathogens. Safety issues needed to be addressed for the biocontrol of Shiga toxin-producing Escherichia coli (STEC) include: lysogenic conversion, Shiga toxin production through phage induction, and emergence/proliferation of bacteriophage insensitive mutants (BIMs). To address these issues, two new lytic phages, vB EcoS Ace (Ace) a...


Microbiome definition re-visited: old concepts and new challenges

Berg, Gabriele; Rybakova, Daria; Fischer, Doreen; Cernava, Tomislav; Vergès, Marie-Christine Champomier; Charles, Trevor; Chen, Xiaoyulong

The field of microbiome research has evolved rapidly over the past few decades and has become a topic of great scientific and public interest. As a result of this rapid growth in interest covering different fields, we are lacking a clear commonly agreed definition of the term ``microbiome.'' Moreover, a consensus on best practices in microbiome research is missing. Recently, a panel of international experts dis...


Organohalide-respiring Desulfoluna species isolated from marine environments

Peng, Peng; Goris, Tobias; Lu, Yue; Nijsse, Bart; Burrichter, Anna; Schleheck, David; Koehorst, Jasper J.; Liu, Jie; Sipkema, Detmer

The genus Desulfoluna comprises two anaerobic sulfate-reducing strains, D. spongiiphila AA1T and D. butyratoxydans MSL71T, of which only the former was shown to perform organohalide respiration (OHR). Here we isolated a third strain, designated D. spongiiphila strain DBB, from marine intertidal sediment using 1,4-dibromobenzene and sulfate as the electron acceptors and lactate as the electron donor. Each strain...



Addressing global ruminant agricultural challenges through understanding the ru...

Mantovani, Hilario C.; Huws, Sharon A.; Creevey, Christopher J.; Oyama, Linda B.; Mizrahi, Itzhak; Denman, Stuart E.; Popova, Milka

The rumen is a complex ecosystem composed of anaerobic bacteria, protozoa, fungi, methanogenic archaea and phages. These microbes interact closely to breakdown plant material that cannot be digested by humans, whilst providing metabolic energy to the host and, in the case of archaea, producing methane. Consequently, ruminants produce meat and milk, which are rich in high-quality protein, vitamins and minerals, ...

Date: 2019   |   Origin: Oasisbr

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