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Development of an amplicon-based sequencing approach in response to the global ...

Chen, Nicholas F.G.; Chaguza, Chrispin; Gagne, Luc; Doucette, Matthew; Smole, Sandra; Buzby, Erika; Hall, Joshua; Ash, Stephanie; Harrington, Rachel

The 2022 multicountry mpox outbreak concurrent with the ongoing Coronavirus Disease 2019 (COVID-19) pandemic further highlighted the need for genomic surveillance and rapid pathogen whole-genome sequencing. While metagenomic sequencing approaches have been used to sequence many of the early mpox infections, these methods are resource intensive and require samples with high viral DNA concentrations. Given the at...

Date: 2023   |   Origin: Repositório Científico do Instituto Nacional de Saúde
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