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A comprehensive method for the sequential separation of extracellular xylanases...

Rodríguez-Sanz, Andrea; Fuciños, Clara; Soares, Célia Maria Gonçalves; Torrado, Ana M.; Lima, Nelson; Rúa, María L.

Several fungal species produce diverse carbohydrate-active enzymes useful for the xylooligosaccharide biorefinery. These enzymes can be isolated by different purification methods, but fungi usually produce other several compounds which interfere in the purification process. So, the present work has three interconnected aims: (i) compare -xylosidase production by Fusarium pernambucanum MUM 18.62 with other crop ...


Isolation of filamentous fungi from different food matrices from Angola and Moz...

Dias, Teresa Vale; Matusse, Claudio; Carvalho, Susana; Silva, Beatriz; Soares, Célia Maria Gonçalves; Lucamba, Zelda; Afonso, Sandra

Agriculture remains the main economic activity in most African countries. However, crops are often contaminated with fungi that can cause diseases or produce mycotoxins, which is a major concern to food safety and security. Little is known about the mycotoxigenic fungi contaminating the most relevant staples in Mozambique and Angola. The aim of this work was to isolate and identify fungi from three food commodi...


Implementation of cutting-edge technologies for the benefit of culture collecti...

Santos, Carla Isabel Arcanjo; Soares, Célia Maria Gonçalves; Venâncio, Armando; Lima, Nelson

Since 1890, microbial culture collections (CCs) have been providing services to the scientific community, acting as reservoirs and providers of microorganisms, including their living cells, genomes, and information, being key players for the development of new and more sustainable products, compounds, and practices. For this reason, the Organisation for Economic Cooperation and Development (OECD) recognized CCs...


Is hand disinfectant a public health problem? A case study in the Centre of Bio...

Dias, Teresa Vale; Soares, Célia Maria Gonçalves; Carvalho, Susana; Lima, Nelson; Venâncio, Armando

For about three years, the world was faced with the CoVID-19 pandemic that showed us the threats of unknown microorganisms. At the beginning of the pandemic several behavioural rules for prevention and protection were imposed. Besides the use of masks, ethanol solutions for hand disinfection were one of the most common measures. Public recipients of alcohol were seen and accessible in every public and private i...


Aflatoxins and ochratoxin A contamination during Merkén Pepper Powder productio...

Costa, Jéssica; Santos, Carla Isabel Arcanjo; Soares, Célia Maria Gonçalves; Lima, Nelson; Santos, Cledir

Berry fruits of Capsicum annuum L. cv. "Cacho de Cabra are used for the manufacture of a traditional pepper powder known as Merkén, which is a Chilean spicy widely consumed at national level in Chile and also exported to different countries. Merkén contamination with Aflatoxins (AFs) and Ochratoxin A (OTA) is a main concerning for local producers and national and worldwide consumers. In the present study, AFs a...


Occurrence of aflatoxins and Ochratoxin A during merkén pepper powder productio...

Costa, Jéssica; Santos, Carla Isabel Arcanjo; Soares, Célia Maria Gonçalves; Rodriguez, R.; Lima, Nelson; Santos, Cledir

The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below. Beta-tubulin (benA) partial sequences: https://www.ncbi.nlm.nih.gov/search/all/?term=, OM400555-OM400584; Internal Transcribed Spacer (ITS) partial sequences: https://www.ncbi.nlm.nih.gov/search/all/?term=, OM307396-OM307401.; Berry fruits of Capsicum ann...


Identificação da micobiota isolada de medronho (Arbutus unedo L.)

Domingues, Joana Margarida Dias; Santos, Carla Isabel Arcanjo; Soares, Célia Maria Gonçalves; Lima, Nelson; Gonçalves, José Carlos

Arbutus unedo L. é uma espécie autóctone em Portugal, com grande capacidade de resistência em solos pobres e áridos, e com facilidade de crescimento em zonas pós-incêndio. O medronho tem diversas aplicações como a produção de aguardente, o consumo do fruto fresco, compotas e outras utilizações culinárias (p. ex., farinha de medronho). No sentido de prolongar o tempo de vida útil de frutos pós-colheita, têm vind...


The effect of calcium concentration on culture medium for the production of bio...

Castañon, Carolina; Soares, Célia Maria Gonçalves; Lima, Nelson; Camões, Aires

Microbial induced calcium carbonate (CaCO3) precipitation (MICCP) is a promising technique for the production of building materials with lower environmental impact than traditional building materials. Sand cementation through microbial activity is a process that uses a microbial agent and nutritive medium as a source of calcium that enables the production of CaCO3, which is used as an agglomerating factor for s...


Penicillium crustosum as a potential OTA producer - new insights from whole - g...

Santos, Carla Isabel Arcanjo; Pinheiro, Miguel; Dias, Teresa; Soares, Célia Maria Gonçalves; Moura, Gabriela M. R. de; Lima, Nelson

Ochratoxin A (OTA) is a well-studied mycotoxin that poses severe health risks. OTA is mainly produced by Aspergillus and Penicillium species associated with food spoilage and it is present in a wide diversity of food and feed products. Recent studies have reported the presence of OTA in food matrices where known OTA producers are not present1,2. For that reason, other species such as P. crustosum are now being ...


Development of a standard operation procedure (SOP) for the polyphasic identifi...

Afonso, Kelly de Almeida; Soares, Célia Maria Gonçalves; Lima, Nelson

The identification of dermatophytes and other clinically relevant filamentous fungi has been essential for a rapid diagnosis and the preservation of authentic microorganisms in culture collections. The aim of this work was the development of a Standard Operating Procedure (SOP) for the polyphasic identification of clinically important species that might be deposited in Micoteca da Universidade do Minho (MUM) an...


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