14 documents found, page 1 of 2
Production of Human Milk Fat Substitutes by Lipase-Catalyzed Acidolysis: Immobi...
Soares, Cleide M. F.; Barbosa, Milson S.; Santos, Samuel B.; Mattedi, Silvana; Lima, Álvaro S.; Pereira, Matheus M.; Tecelão, CarlaHuman milk fat (HMF) triacylglycerols (TAGs) mainly contain palmitic acid esterified at the sn-2 position while oleic and other unsaturated fatty acids are located at positions sn-1,3. This study aimed at the production of HMF substitutes (HMFS) by lipase-catalyzed acidolysis of tripalmitin with oleic acid, in a solvent-free medium. Burkholderia cepacia lipase (BCL) was immobilized in silica (prepared with prot...
Continuous production of dietetic structured lipids using crude acidic Olive po...
Souza-Gonçalves, Joana; Fialho, Arsénio; Soares, Cleide M. F.; Osório, Natália M.; Ferreira-Dias, SuzanaCrude olive pomace oil (OPO) is a by-product of olive oil extraction. In this study, lowcalorie structured triacylglycerols (TAGs) were produced by acidolysis of crude OPO with mediumchain fatty acids (caprylic, C8:0; capric, C10:0) or interesterification with their ethyl ester forms (C8EE, C10EE). These new TAGs present long-chain fatty acids (L) at position sn-2 and medium-chain fatty acids (M) at positions s...
Production of human milk fat substitutes by lipase-catalyzed acidolysis: immobi...
Soares, Cleide M. F.; Barbosa, Milson S.; Santos, Samuel B.; Mattedi, Silvana; Lima, Álvaro S.; Pereira, Matheus M.; Tecelão, CarlaHuman milk fat (HMF) triacylglycerols (TAGs) mainly contain palmitic acid esterified at the sn-2 position while oleic and other unsaturated fatty acids are located at positions sn-1,3. This study aimed at the production of HMF substitutes (HMFS) by lipase-catalyzed acidolysis of tripalmitin with oleic acid, in a solvent-free medium. Burkholderia cepacia lipase (BCL) was immobilized in silica (prepared with prot...
Pre-treatment strategies based on aqueous two-phase systems comprising ionic li...
Pereira, Matheus M.; Brito, Larissa L. R.; de Miranda, Rita de C. M.; de Souza, Ranyere Lucena; Soares, Cleide M. F.; Coutinho, João A. P.ancer remains the cause of millions of deaths every year worldwide, and reliable early-stage diagnosis methods are essential to prevent them. Cancer biomarkers quantification in human fluids has gained relevance in detecting cancer in an early-stage. Among these, the levels of the biomarkers epinephrine, norepinephrine and vanillylmandelic acid in urine have been used for the diagnosis of adrenal cancer. Howeve...
Antimycotic nail polish based on humic acid-coated silver nanoparticles for ony...
Dantas, Ketheleen N. M.; Andrade, Lucas R.; Lisboa, Erika; Santana, Victoria L.; Dos Santos, André L. S.; Mello, Thaís P.; Sangenito, Leandro S.Background: The objective of this work has been the development of silver nanoparticles (AgNPs) coated with humic acid (HA) and their incorporation into an enamel for antifungal activity. AgNPs were synthesized by chemical reduction using sodium borohydride (NaBH4) as a reducing agent and coated with HA. Uncoated AgNPs were synthesized as control. AgNPs and HAAgNPs were characterized by ultravioletvisible spect...
Selective partition of caffeine from coffee bean and guarana seed extracts usin...
Santos, Samuel B.; Reis, Igor A. O.; Silva, Camila P. C.; Campos, Andressa F.; Ventura, Sonia P. M.; Soares, Cleide M. F.; Lima, Alvaro S.This work proposes the application of flexible alcoholic aqueous two-phase systems to manipulate the partition of caffeine. An optimization study was performed the caffeine partitioning towards top phase (60.0 wt% 2-propanol + 20.0 wt% K3PO4 at 303 K - K-caf = 3.4 and R-T = 89.1%) and bottom phase (60.0 wt% methanol + 17.5 wt% K2HPO4/KH2PO4 at 278 K - K-caf = 0.06 and R-B = 81.1%). These processes were evaluate...
Ionic Liquid-Based Aqueous Biphasic Systems as a Versatile Tool for the Recover...
Santos, Joao H.; e Silva, Francisca A.; Ventura, Sonia P. M.; Coutinho, Joao A. P.; de Souza, Ranyere L.; Soares, Cleide M. F.; Lima, Alvaro S.The comparative evaluation of distinct types of ionic liquid-based aqueous biphasic systems (IL-ABS) and more conventional polymer/salt-based ABS to the extraction of two antioxidants, eugenol and propyl gallate, is focused. In a first approach, IL-ABS composed of ILs and potassium citrate (C6H5K3O7/C6H8O7) buffer at pH 7 were applied to the extraction of two antioxidants, enabling the assessment of the impact ...
Lipase purification using ionic liquids as adjuvants in aqueous two-phase systems
Souza, Ranyere L.; Ventura, Sonia P. M.; Soares, Cleide M. F.; Coutinho, Joao A. P.; Lima, Alvaro S.Aqueous two-phase systems (ATPS) are efficient, environmentally friendly, and
Aqueous two-phase systems based on cholinium salts and tetrahydrofuran and thei...
Souza, Ranyere L.; Lima, Rafaella A.; Coutinho, Joao A. P.; Soares, Cleide M. F.; Lima, Alvaro S.Aqueous two-phase systems (ATPS) formed with cholinium-based ionic liquid - ILs (or salts) are a novel, low cost, and high efficient technique for the recovery of biomolecules. This study examines the formation of ATPS based on cholinium-based salts (cholinium chloride, cholinium bitartrate and cholinium dihydrogencitrate) and tetrahydrofuran (THF) for the purification of lipase from Bacillus sp. ITP-001, produ...
Novel aqueous two-phase systems based on tetrahydrofuran and potassium phosphat...
Souza, Ranyere L.; Lima, Rafaella A.; Coutinho, Joao A. P.; Soares, Cleide M. F.; Lima, Alvaro S.Aqueous two-phase systems (ATPS) based on tetrahydrofuran (THF) + potassium phosphate buffer (pH 7) were used in this work for the purification of lipases. Binodal curve, tie lines and critical point were obtained for the new THF-salt ATPS at 25 degrees C and the binodal curve were successfully correlated with the Merchuck and Hu equations. To optimize the extraction capability of this ATPS the effects of the c...