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Nematicidal activity of Paecilomyces marquandii proteases on infective larvae o...

Genier,Hugo Leonardo André; Queiroz,José Humberto de; Braga,Fabio Ribeiro; Soares,Filippe Elias de Freitas; Araújo,Jackson Victor de

ABSTRACT The present study aimed to evaluate the action of Paecilomyces marquandii proteases on Ancylostoma spp L3. White halos in the zymogram confirmed the proteolytic action. Difference (p <0.01) between the number of L3 in the differents groups was found, with 41.4% of reduction of Ancylostoma spp. L3 before 24 hours.

Date: 2016   |   Origin: Oasisbr

In vitro predatory activity of conidia of fungal isolates of the Duddingtonia f...

Braga,Fabio Ribeiro; Araujo,Juliana Milani; Araújo,Jackson Victor de; Soares,Filippe Elias de Freitas; Tavela,Alexandre de Oliveira; Frassy,Luiza Neme

INTRODUCTION: Angiostrongylus vasorum is a nematode that parasitizes molluscs, dogs, and even man. METHODS: The objective was to evaluate the predatory activity of the conidia of two fungal isolates of Duddingtonia flagrans (AC001 and CG722) on first-stage larvae (L1) of A. vasorum in laboratory conditions. RESULTS: At the end of the experiment, there were significant reductions (p<0.01) of 74.5% and 63.2%, on ...

Date: 2013   |   Origin: Oasisbr

Proteolytic action of the crude extract ofDuddingtonia flagrans on cyathostomin...

Braga,Fabio Ribeiro; Araújo,Jackson Victor de; Soares,Filippe Elias de Freitas; Araujo,Juliana Milani; Ferreira,Sebastião Rodrigo

The objective of this study was to examine the action of the crude extract of Duddingtonia flagrans (isolates AC001 and CG722) on infective larvae (L3) of cyathostomins in coprocultures and to confirm its proteolytic activity by means of a zymogram. The following groups were formed in coprocultures: Group 1: 10 mL of crude extract of D. flagrans (AC001); group 2: 10 mL of crude extract of AC001 with 10 mM of Ca...

Date: 2013   |   Origin: Oasisbr

First report of interaction of nematophagous fungi onLibyostrongylus douglassii...

Braga,Fabio Ribeiro; Araújo,Jackson Victor; Tavela,Alexandre de Oliveira; Vilela,Vinicius Longo Ribeiro; Soares,Filippe Elias de Freitas

Libyostrongylus douglassii is a gastrointestinal nematode parasite of ostriches that can cause up to 50% mortality in young birds. The objective of this study was to compare the predatory capacity of two isolates of the predatory fungi Duddingtonia flagrans(AC001 and CG722 isolates) and one of Arthrobotrys cladodes (CG719) on infective larvae (L3) of L. douglassii under laboratory conditions, in 2% water-agar m...

Date: 2013   |   Origin: Oasisbr

Optimization of protease production by the fungusMonacrosporium thaumasium and ...

Soares,Filippe Elias de Freitas; Braga,Fabio Ribeiro; Araújo,Jackson Victor de; Lima,Walter dos Santos; Mozzer,Lanuze Rose; Queiroz,José Humberto de

The objectives of this study were to optimize protease production from the nematophagous fungus Monacrosporium thaumasium (NF34a) and evaluate its larvicidal activity and biological stability. An isolate of the nematophagous fungus Monacrosporium thaumasium (NF34a) was used to produce the enzyme. The Plackett-Burman design was used in order to scan which components of the culture medium could have a significant...

Date: 2013   |   Origin: Oasisbr

Control of Strongyloides westeri by nematophagous fungi after passage through t...

Araujo,Juliana Milani; Araújo,Jackson Victor de; Braga,Fabio Ribeiro; Tavela,Alexandre de Oliveira; Ferreira,Sebastião Rodrigo

Strongyloides westeri is the most prevalent nematode among equines aged up to four months and causes gastrointestinal disorders. The objective of this study was to observe the control of infective S. westeri larvae (L3) by the nematophagous fungi Duddingtonia flagrans (AC001) and Monacrosporium thaumasium (NF34) after passage through the gastrointestinal tract of female donkeys. Twelve dewormed female donkeys t...

Date: 2012   |   Origin: Oasisbr

Atividade larvicida do extrato bruto enzimático do fungo Duddingtonia flagras s...

Braga,Fabio Ribeiro; Araujo,Juliana Milani; Tavela,Alexandre de Oliveira; Araújo,Jackson Victor de; Soares,Filippe Elias de Freitas

INTRODUÇÃO: Angiostrongylus vasorum é um nematóide que parasita cães domésticos e eventualmente o homem. MÉTODOS: O objetivo deste trabalho foi observar a atividade predatória in vitro do extrato bruto enzimático do fungo Duddingtonia flagrans sobre larvas de primeiro estádio A. vasorum em condições laboratoriais no meio ágar-água 2%. RESULTADOS: Ao final do experimento, os percentuais de redução das L1 de A. v...

Date: 2011   |   Origin: Oasisbr

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