12 documents found, page 1 of 2
SCI1 Is a Direct Target of AGAMOUS and WUSCHEL and Is Specifically Expressed in...
Cruz, Joelma O.; San Martin, Juca A.B.; Lubini, Greice; Strini, Edward J.; Sobral, Rómulo Sacramento; Pinoti, Vitor F.; Ferreira, Pedro B.The specified floral meristem will develop a pre-established number of floral organs and, thus, terminate the floral meristematic cells. The floral meristematic pool of cells is controlled, among some others, by WUSCHEL (WUS) and AGAMOUS (AG) transcription factors (TFs). Here, we demonstrate that the SCI1 (Stigma/style cell-cycle inhibitor 1) gene, a cell proliferation regulator, starts to be expressed since th...
The dynamics of flower development in Castanea sativa Mill
Alhinho, Ana Teresa Talhinhas Barata; Ramos, Miguel Jesus Nunes; Alves, Sofia; Rocheta, Margarida; Morais-Cecílio, Leonor; Gomes-Laranjo, JoséThe sweet chestnut tree (<i>Castanea sativa</i> Mill.) is one of the most significant Mediterranean tree species, being an important natural resource for the wood and fruit industries. It is a monoecious species, presenting unisexual male catkins and bisexual catkins, with the latter having distinct male and female flowers. Despite the importance of the sweet chestnut tree, little is known regarding the molecul...
Genome-wide identification of epigenetic regulators in Quercus suber L.
Silva, Helena G.; Sobral, Rómulo Sacramento; Magalhães, Alexandre P.; Morais-Cecílio, Leonor; Costa, Maria Manuela RibeiroModifications of DNA and histones, including methylation and acetylation, are critical for the epigenetic regulation of gene expression during plant development, particularly during environmental adaptation processes. However, information on the enzymes catalyzing all these modifications in trees, such as <i>Quercus suber</i> L., is still not available. In this study, eight DNA methyltransferases (DNA Mtases) a...
Evolutionary conservation of the orchid MYB transcription factors DIV, RAD, and...
Valoroso, Maria Carmen; Sobral, Rómulo Sacramento; Saccone, Giuseppe; Salvemini, Marco; Costa, Maria Manuela Ribeiro; Aceto, SerenaThe MYB transcription factors DIVARICATA (DIV), DIV-and-RAD-Interacting-Factor (DRIF), and the small interfering peptide RADIALIS (RAD) can interact, forming a regulatory module that controls different plant developmental processes. In the snapdragon Antirrhinum majus, this module, together with the TCP transcription factor CYCLOIDEA (CYC), is responsible for the establishment of floral dorsoventral asymmetry. ...
Successive domain rearrangements underlie the evolution of a regulatory module ...
Raimundo, João Alexandre Pereira; Sobral, Rómulo Sacramento; Laranjeira, Sara; Costa, Maria Manuela RibeiroThe establishment of new interactions between transcriptional regulators increases the regulatory diversity that drives phenotypic novelty. To understand how such interactions evolve, we have studied a regulatory module (DDR) composed by three MYB-like proteins: DIVARICATA (DIV), RADIALIS (RAD), and DIV-and-RAD-Interacting Factor (DRIF). The DIV and DRIF proteins form a transcriptional complex that is disrupted...
Molecular characterization of flower development of Quercus suber L
Sobral, Rómulo SacramentoQuercus suber, or cork oak, is an important forest species in Southern Europe and Northern Africa. This perennial monoecious species has an accentuated protandrous system: in late winter, and sometimes in autumn, male flowers develop in catkins that emerge close to the dormant buds. Female flowers develop in spring (4 to 8 weeks after male flowering) in a process that takes more than one month to full completio...
Role of floral organ identity genes in the development of unisexual flowers of ...
Sobral, Rómulo Sacramento; Costa, Maria Manuela RibeiroMonoecious species provide an excellent system to study the specific determinants that underlie male and female flower development. Quercus suber is a monoecious species with unisexual flowers at inception. Despite the overall importance of this and other tree species with a similar reproductive habit, little is known regarding the mechanisms involved in the development of their male and female flowers. Here, w...
Antibiotic free selection for the high level biosynthesis of a silk-elastin-lik...
Barroca, Mário Jorge Faria; Rodrigues, Paulo; Sobral, Rómulo Sacramento; Costa, Maria Manuela Ribeiro; Chaves, Susana R.; Machado, RaulSilk-elastin-like proteins (SELPs) are a family of genetically engineered recombinant protein polymers exhibiting mechanical and biological properties suited for a wide range of applications in the biomedicine and materials fields. They are being explored as the next generation of biomaterials but low productivities and use of antibiotics during production undermine their economic viability and safety. We have ...
The quest for molecular regulation underlying unisexual flower development
Sobral, Rómulo Sacramento; Silva, Helena G; Morais-Cecílio, Leonor; Costa, Maria Manuela RibeiroThe understanding of the molecular mechanisms responsible for the making of a unisexual flower has been a long-standing quest in plant biology. Plants with male and female flowers can be divided mainly into two categories: dioecious and monoecious, and both sexual systems co-exist in nature in ca of 10% of the angiosperms. The establishment of male and female traits has been extensively described in a hermaphro...
Comparative transcriptomic analysis of male and female flowers of monoecious Qu...
Rocheta, Margarida; Sobral, Rómulo Sacramento; Magalhães, Joana; Amorim, Maria I.; Ribeiro, Teresa; Pinheiro, Miguel; Egas, ConceiçãoMonoecious species provide a comprehensive system to study the developmental programs underlying the establishment of female and male organs in unisexual flowers. However, molecular resources for most monoecious non-model species are limited, hampering our ability to study the molecular mechanisms involved in flower development of these species. The objective of this study was to identify differentially express...