25 documents found, page 1 of 3
Correlation of maternal concentrations of plasma testosterone with fetal sex in...
Busato,Eduarda Maciel; Weiss,Romildo Romualdo; Abreu,Ana Claudia Machinski Rangel de; Bergstein-Galan,Tácia Gomes; Marcondes,Felipe Augusto BeckertABSTRACT: The objectives of this study were to evaluate the correlation of fetal sex and plasma testosterone concentrations between the 5th and 8th months of pregnancy in mares and to verify the applicability of this test to predict fetal sex. Blood samples were collected from 21 mares at 30-day intervals of between 150 and 240 days of pregnancy. Plasma testosterone was determined by radioimmunoassay and the se...
Guinea Pigs Naturally Infected by Leishmania enriettii: Clinical Analyses, Para...
Soccol,Vanete Thomaz; Castro,Edilene Alcântara de; Reifur,Larissa; Teixeira,Valéria Natascha; Lange,Rogério Ribas; Luz,EnnioAbstract Leishmania enriettii has only been found in Curitiba metropolitan region, southern Brazil were it was first observed in Cavia porcellus from the vivarium of Instituto de Biologia e Pesquisas Tecnológicas (IBPT - today named TECPAR) by Medina, 1944. Despite more than a half century from its discovery and several research articles on this species, the natural clinical signs in guinea pigs and the parasit...
A Review on COVID-19 Diagnosis Tests Approved for Use in Brazil and the Impact ...
Vedova-Costa,Jean Michel Dela; Ramos,Eliézer Lucas Pires; Boschero,Raphael Aparecido; Ferreira,Gabriela Nascimento; Soccol,Vanete ThomazAbstract With the COVID-19 pandemic, many diagnostic tests (molecular or immunological) were rapidly standardised, given the urgency of the situation, many are still in the process of being validated. The main objective of this study was to review the aspects of the diagnostic kits approved in Brazil and their application in the different federative units to gather epidemiological information. In order to achie...
Probability of occurrence of the Brazilian spotted fever in northeast of Paraná...
Otomura,Flávio Haragushiku; Truppel,Jessé Henrique; Moraes Filho,Jonas; Labruna,Marcelo Bahia; Rossoni,Diogo Francisco; Massafera,RubensAbstract Brazilian spotted fever (BSF) is a fatal zoonosis because of the difficulties in its early diagnosis and treatment. Occurrences of BSF in the northeast of the state of Paraná prompted investigation of areas at risk of this rickettsiosis in the municipalities of Japira, Jaboti, Pinhalão and Tomazina. To determine the areas at risk, 592 serum samples from dogs and 230 from equids were analyzed by means o...
Bovine Herpesvirus 4 in Parana State, Brazil: case report, viral isolation, and...
Kruger,Ernesto Renato; Penha,Tania Regina; Stoffelo,Daura Regina Eira; Roehe,Paulo Michel; Ribeiro,Magda Costa; Soccol,Vanete ThomazBovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinaesub-family and belongs to genus Rhadinovirus. This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. The goal of this work was to characterize a virus strain isolate from a cow’s uterine outflow. From swabs drawn of ute...
Quality Control of Biotechnological Inputs DetectingMycoplasma
Netto,Cristiane; Soccol,Vanete Thomaz; Sepulveda,Lya; Garcia,Gabriel Henrique Oliveira; Timenetsky,JorgeThe aim of this work was to study the Polymerase Chain Reaction (PCR) as a tool of quality control of bovine sera and cellular cultures used in the biotechnological industry. A total of 46 samples of bovine sera derived from two slaughterhouses and 33 samples of BHK21 cells derived from two biotechnological industries were evaluated using the primers GPO-3 (sense) and MGSO (antisense). The PCR technique sensibi...
Torularhodin and Torulene: Bioproduction, Properties and Prospective Applicatio...
Zoz,Liliana; Carvalho,Julio Cesar; Soccol,Vanete Thomaz; Casagrande,Thais Costa; Cardoso,LigiaTorularhodin and torulene are two widespread microbial carotenoids with relatively few studies, as compared to other nutraceutical carotenoids such as β-carotene, lycopene and astaxanthin. Several genera of microorganisms produce it in high concentration (up to 0.1% of the cell dry weight), probably as a protection against photooxidation and free radicals. These pigments, which differ by a terminal carboxylic g...
Experimental infection of BHK21 and Vero cell lines with different Mycoplasma spp.
Netto,Cristiane; Soccol,Vanete Thomaz; Sepulveda,Lya Madureira; Timenetsky,JorgeMycoplasma spp, belongs to the class Mollicutes and is capable to produce alterations in cellular cultures causing damages to the biotechnological industry. Bioproducts generally require two essential inputs, bovine serum and cells. The study herein aims to evaluate the mycoplasma concentrations that affect the growing of BHK21 and Vero cells. The species used were: Mycoplasma orale, M. salivarium, M. arginini ...
Evaluation of probiotic properties of Pediococcus acidilactici B14 in associati...
Ribeiro,Maria Carolina de Oliveira; Vandenberghe,Luciana Porto de Souza; Spier,Michele Rigon; Paludo,Katia Sabrina; Soccol,Carlos RicardoThe aim of this study was to evaluate the probiotic properties of Pediococcus acidilactici B14 and to study its resistance in the gastrointestinal system when combined with Lactobacillus acidophilus ATCC 4356 and used in a potentially symbiotic aerated soy based dessert. P. acidilactici B14 showed some important probiotic characteristics such as survival rate of 45.9% at pH 2.5; 72.4% in 0.3% bile salts and 95....
Polymerase chain reaction and nested-PCR approaches for detecting Cryptosporidi...
Osaki,Silvia Cristina; Soccol,Vanete Thomaz; Costa,Adriana Oliveira; Oliveira-Silva,Marcia Benedita; Pereira,Juliana Tracz; Procopio,Antonio EduardoIntroduction Cryptosporidium is an important protozoan cause of waterborne disease worldwide of concern to public health authorities. To prevent outbreaks of cryptosporidiosis, the monitoring of this parasite in drinking water is necessary. In the present work, the polymerase chain reaction (PCR) and nested-PCR techniques were used to detect Cryptosporidium in raw water from catchment points of four water treat...