5 documents found, page 1 of 1
Molecular mechanisms beyond glucose transport in diabetes-related male infertility
Alves, M. G.; Martins, A. D.; Rato, L.; Moreira, P. I.; Socorro, S.; Oliveira, P. F.Diabetes mellitus (DM) is one of the greatest public health threats in modern societies. Although during a few years it was suggested that DM had no significant effect in male reproductive function, this view has been challenged in recent years. The increasing incidence of DM worldwide will inevitably result in a higher prevalence of this pathology in men of reproductive age and subfertility or infertility asso...
Identification of androgen receptor variants in testis from humans and other ve...
Laurentino, S. S.; Pinto, Patricia IS; Tomas, J.; Cavaco, J. E. B.; Sousa, M.; Barros, A.; Power, Deborah; Canario, Adelino V. M.; Socorro, S.The androgen receptor (AR) is a ligand-activated transcription factor member of the nuclear receptor superfamily. The existence of alternatively spliced variants is well recognised for several members of this superfamily, most of them having functional importance. For example, several testicular oestrogen receptor variants have been suggested to play a role in the regulation of spermatogenesis. However, informa...
Structure, tissue distribution and estrogen regulation of splice variants of th...
Pinto, Patricia IS; Teodósio, H. R.; Socorro, S.; Power, Deborah; Canario, Adelino V. M.Estrogen actions are mainly mediated by specific nuclear estrogen receptors (ERs), for which different genes and a diversity of transcript variants have been identified, mainly in mammals. In this study, we investigated the presence of ER splice variants in the teleost fish gilthead sea bream (Sparus auratus), by comparison with the genomic organization of the related species Takifugu rubripes. Two exon2-delete...
Immunohistochemical detection of estrogen receptors in fish scales
Pinto, Patricia IS; Estêvão, M. Dulce; Redruello, Begoña; Socorro, S.; Canario, Adelino V. M.; Power, DeborahCalcium mobilization from internal stores, such as scales, induced by 17b-estradiol during sexual maturation in salmonids is well documented. This calcium mobilization from scales is proposed to be mediated by the estrogen receptor (ER). However, the ER subtypes involved and signaling mechanisms responsible for this effect remain to be fully characterized. In the present study, we have localized ERa, ERba and E...
A cDNA for European sea bass (Dicentrachus labrax) 11 beta-hydroxylase: Gene ex...
Socorro, S.; Martins, Rute S. T.; Deloffre, Laurence A. M.; Mylonas, Constantinos C.; Canario, Adelino V. M.Steroid P450 11 -hydroxylase, encoded by the CYP11B gene, is a key mitochondrial enzyme for the production of 11-oxygenated androgens, which have been shown to be potent masculinising steroids in several Wsh species. In this study we have isolated a CYP11B cDNA of 1903 base pairs from the testis of European sea bass (Dicentrarchus labrax) encoding a predicted protein of 552 amino acids. The amino acid identitie...