8 documents found, page 1 of 1
An in vivo model of ischemic stroke to study potential pharmacological targets
Mendes, Priscila; Solas, João; Santos, Sofia; Boralho, Adelaide; Ratilal, Bernardo; Mateus, Vanessa; Rocha, JoãoBackground: Ischemic Stroke (IS) is caused by a focal disruption in cerebral blood flow due to the occlusion of a blood vessel, most commonly the middle cerebral artery (MCA) [1]. The ischemic cascade results in neuronal death and inflammatory response, including in situ microglia activation. Inflammation is a double‐edged sword; it can cause tissue injury but can also help in the tissue repair of the brain [2]...
An in vivo model of ischemic stroke to study potential pharmacological targets
Mendes, Priscila; Solas, João; Santos, Sofia; Fernandes, Adelaide; Ratilal, Bernardo; Mateus, Vanessa; Rocha, JoãoBackground: Ischemic Stroke (IS) is caused by a focal disruption in cerebral blood flow due to the occlusion of a blood vessel, most commonly the middle cerebral artery (MCA)—the ischemic cascade results in neuronal death and inflammatory response, including in situ microglia activation. Inflammation is a double‐edged sword; it can cause tissue injury but can also help in the tissue repair of the brain. The onl...
Chronic experimental model of TNBS-induced colitis to study inflammatory bowel ...
Silva, Inês; Solas, João; Pinto, Rui; Mateus, VanessaBackground: Inflammatory bowel disease (IBD) is a world healthcare problem. In order to evaluate the effect of new pharmacological approaches for IBD, we aim to develop and validate chronic trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. Methods: Experimental colitis was induced by the rectal administration of multiple doses of TNBS in female CD-1 mice. The protocol was performed with six experime...
A proposed lectin‐mediated mechanism to explain the in Vivo antihyperglycemic a...
Grácio, Madalena; Rocha, João; Pinto, Rui; Boavida Ferreira, Ricardo; Solas, João; Eduardo‐Figueira, Maria; Sepodes, Bruno; Ribeiro, Ana CristinaExperiments conducted in vitro and in vivo, as well as clinical trials for hypoglycemic therapeutics, support the hypoglycemic properties of the lectin γ-conglutin, a Lupinus seed storage protein, by a mechanism not yet been clarified. Structural studies established that binding of γ-conglutin, in native and denatured form, to insulin occurs by a strong binding that resists rupture when 0.4 M NaCl and 0.4 M gal...
A proposed lectin-mediated mechanism to explain the in vivo antihyperglycemic a...
Grácio, Madalena; Rocha, João; Pinto, Rui; Ferreira, Ricardo Boavida; Solas, João; Eduardo-Figueira, Maria; Sepodes, Bruno; Ribeiro, Ana CristinaExperiments conducted in vitro and in vivo, as well as clinical trials for hypoglycemic therapeutics, support the hypoglycemic properties of the lectin γ-conglutin, a Lupinus seed storage protein, by a mechanism not yet been clarified. Structural studies established that binding of γ-conglutin, in native and denatured form, to insulin occurs by a strong binding that resists rupture when 0.4 M NaCl and 0.4 M gal...
Reduction of inflammation and colon injury by a Pennyroyal phenolic extract in ...
Rocha, João; Direito, Rosa; Lima, Ana; Mota, Joana; Gonçalves, Margarida; Duarte, Maria Paula; Solas, João; Peniche, Bruno Felício; Fernandes, AdelaidePurpose - Little is known about the pharmacological effects of the phenolic compounds of Pennyroyal (Mentha pulegium). This Mediterranean aromatic plant, used as a gastronomic spice and as food preservative by the food industry has been studied mainly due to its essential oil antibacterial properties, composed primarily by monoterpenes. With this work, we aimed to evaluate the effects of a phenolic extract of p...
TNBS-induced colitis in rodents: preliminary results of a chronic model
Silva, Inês; Solas, João; Sousa, Mariana; Modesto, Rita; Mateus, Vanessa; Pinto, RuiChronic inflammatory disease of the gastrointestinal (GI) tract characterized by recurrent ulceration. Treatment of inflammatory bowel disease induce and maintain the patient in remission However do not modify or reverse the underlying pathogenic mechanism. Nonclinical studies for emerging therapeutic strategies. Aim of the study: Evaluate the efficacy of new drugs in inflammatory bowel disease through an anima...
Reduction of inflammation and colon injury by a Pennyroyal phenolic extract in ...
Rocha, João; Direito, Rosa; Lima, Ana; Mota, Joana; Gonçalves, Margarida; Duarte, Maria Paula; Solas, João; Peniche, Bruno Felício; Fernandes, AdelaidePurpose: Little is known about the pharmacological effects of the phenolic compounds of Pennyroyal (Mentha pulegium). This Mediterranean aromatic plant, used as a gastronomic spice and as food preservative by the food industry has been studied mainly due to its essential oil antibacterial properties, composed primarily by monoterpenes. With this work, we aimed to evaluate the effects of a phenolic extract of pe...