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Metagenomic sequencing with spiked primer enrichment for viral diagnostics and ...

Deng, X.; Achari, A.; Federman, S.; Yu, G.; Somasekar, S.; Bártolo, I.; Yagi, S.; Mbala-Kingebeni, P.; Kapetshi, J.; Ahuka-Mundeke, S.

Metagenomic next-generation sequencing (mNGS), the shotgun sequencing of RNA and DNA from clinical samples, has proved useful for broad-spectrum pathogen detection and the genomic surveillance of viral outbreaks. An additional target enrichment step is generally needed for high-sensitivity pathogen identification in low-titre infections, yet available methods using PCR or capture probes can be limited by high c...


Establishment and cryptic transmission of Zika virus in Brazil and the Americas

Faria, N. R.; Quick, J.; Claro, I. M.; Th?z?, J.; Jesus, J. G. de; Giovanetti, M.; Kraemer, M. U. G.; Hill, S. C.; Black, A.; Costa, A. C. da

University of Oxford. Department of Zoology, Oxford, UK / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.; University of Birmingham. Institute of Microbiology and Infection. Birmingham, UK.; University of Oxford. Department of Zoology. Oxford UK.; University of Oxford. Department of Zoology. Oxford, UK / Harvard Medical School. Boston, MA, USA / Boston C...

Date: 2017   |   Origin: Oasisbr

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