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Effect of surface coating on the biodistribution profile of gold nanoparticles ...

Tiago Morais; Maria Elisa Soares; Jose Alberto Duarte; Leonor Soares; Silvia Maia; Paula Gomes; Eulalia Pereira; Sonia Fraga; Helena Carmo

Successful application of gold nanoparticles (AuNPs) in biomedicine requires extensive safety assessment for which biokinetic studies are crucial. We evaluated the biodistribution of AuNPs (similar to 20 nm) with different surface coatings: citrate, 11-MUA and 3 pentapeptides, CALNN, CALND and CALNS, after i.v. administration to rats (0.6-1 mg Au/kg). Biodistribution was evaluated based on Au tissue content mea...


P2Y receptors on astrocytes and microglia mediate opposite effects in astroglia...

Clara Quintas; Sonia Fraga; Jorge Goncalves; Gloria Queiroz

Nucleotides released upon brain injury signal to astrocytes and microglia playing an important role in astrogliosis, but the participation of microglia in the purinergic modulation of astrogliosis is still unclear. Highly enriched astroglial cultures and co-cultures of astrocytes and microglia were used to investigate the influence of microglia in the modulation of astroglial proliferation mediated by nucleotid...


The P2Y(1) and P2Y(12) receptors mediate autoinhibition of transmitter release ...

Clara Quintas; Sonia Fraga; Jorge Goncalves; Gloria Queiroz

In the sympathetic nervous system, ATP is a co-transmitter and modulator of transmitter release, inhibiting noradrenaline release by acting on P2Y autoreceptors, but in peripheral tissues the subtypes involved have only scarcely been identified. We investigated the identity of the noradrenaline release-inhibiting P2Y subtypes in the epididymal portion of vas deferens and tail artery of the rat. The subtypes ope...


Influence of microglia on nucleotide-induced astroglia proliferation

Gloria Queiroz; Clara Quintas; Diogo Pestana; Sonia Fraga; Jorge Goncalves


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