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Non-canonical amino acids as building blocks for peptidomimetics: structure, fu...

This review provides a fresh overview of non-canonical amino acids and their applications in the design of peptidomimetics. Non-canonical amino acids appear widely distributed in nature and are known to enhance the stability of specific secondary structures and/or biological function. Contrary to the ubiquitous DNA-encoded amino acids, the structure and function of these residues are not fully understood. Here,...

Hofmeister effects on protein stability are dependent on the nature of the unfo...

The interpretation of salt´s effects on protein stability discriminates between low concentration regimes, dominated by ion specific-binding or Debye-Hückel screening, and high concentration regimes, generally described by Hofmeister effects. However, predicting a critical concentration at which one effect overcomes the other is still challenging. Reasonable quantitative estimates can be obtained resorting to s...

The synergic effect of water and biomolecules in intracellular phase separation

Phase separation has long been observed within aqueous mixtures of two or more different compounds, such as proteins, salts, polysaccharides and synthetic polymers. A growing body of experimental evidence indicates that phase separation also takes place inside living cells, where intrinsically disordered proteins and other molecules such as RNA are thought to assemble into membraneless organelles. These structu...

Protein folding and quinary interactions: creating cellular organisation throug...

The marginal stability of globular proteins in the cell is determined by the balance between excluded volume effect and soft interactions. Quinary interactions are a type of soft interactions involved in intracellular organisation and known to have stabilising or destabilising effects on globular proteins. Recent studies suggest that globular proteins have structural flexibility, exhibiting more than one functi...

Partitioning of bovine lactoferrin in aqueous two-phase system containing poly(...

The partitioning of the whey protein lactoferrin, which is an iron transporter glycoprotein, in an aqueous two-phase system composed of poly(ethylene glycol) (PEG) and sodium citrate was evaluated. Equilibrium data at 25 °C were determined for each system studied using PEG with a molar mass of 1000 and 4000 g·mol1 at pH values of 5.5, 6.5, and 7.5. An increase in the molar mass of the polymer promoted the expan...

Role of active species formed by electrolysis on electrochemical therapy

Oxidative modification of proteins induced by electrogenerated HO radicals

Most in vitro studies on the oxidative modifications associated to oxidative stress requires the in situ generation of oxygen radicals. The generation of hydroxyl radicals from the decomposition of hydrogen peroxide by metal cations is widely used for this purpose. It is recognized that results obtained in this way may be influenced by non-­‐specific binding between the metal and the substrate [1]. In this cont...

FEBS 50th Anniversary Virtual Issue: Portugal

This celebratory Virtual Issue for the 50th anniversary of FEBS (2014) highlights the high quality and diversity of biochemistry research carried out in Portugal. Up to 2013, 303 articles were published in FEBS Letters and The FEBS Journal / European Journal of Biochemistry with at least one author having Portugal as an address.

Recovery and partitioning of fibrinolytic protease from Bacillus sp. UFPEDA 485...

Fibrinolytic proteases produced by Bacillus sp. has attracted interest in the pharmaceutical industry as a promising alternative in thrombolytic therapy due to their effectiveness in degrading fibrin, its production requiring the development of an efficient recovery process. Aqueous two-phase systems (ATPS) have been recognized as an efficient and economical process for recovering enzymes. To optimize the recov...

NMR and molecular modelling studies on elastase inhibitor-peptides for wound ma...

Proteases play an important and critical role in the physiological process of wound repair. However, excessive and unregulated release of proteolytic enzymes (e.g., elastase) mediates abnormal degradation of healthy tissues, which leads to inflammatory disorders such as chronic wounds. Thus, it is of therapeutic interest to develop novel synthetic inhibitor-peptides of elastase, which can restore the balance be...