13 documents found, page 1 of 2
Volatilomics as an emerging strategy to determine potential biomarkers of femal...
Brinca, Ana Teresa; Anjos, O.; Alves, Maria Manuel; Sousa, Ângela; Oliani, António Hélio; Breitenfeld, Luísa; Passarinha, Luís A.Due to its high prevalence, infertility has become a prominent public health issue, posing a significant challenge to modern reproductive medicine. Some clinical conditions that lead to female infertility include polycystic ovary syndrome (PCOS), endometriosis, and premature ovarian failure (POF). Follicular fluid (FF) is the biological matrix that has the most contact with the oocyte and can, therefore, be use...
Determinação de potenciais biomarcadores de infertilidade feminina : estudo piloto
Brinca, Ana Teresa; Anjos, O.; Alves, Maria Manuel; Sousa, Ângela; Oliani, António Hélio; Breitenfeld, Luísa; Passarinha, Luís A.Devido à sua elevada incidência, a infertilidade tornou-se uma questão de saúde pública proeminente, representando um desafio significativo para a medicina reprodutiva moderna.
Metallic structures: Effective agents to fight pathogenic microorganisms
Pereira, Diana; Carreira, Tiago Soares; Alves, Nuno; Sousa, Ângela; Valente, Joana F.A.The current worldwide pandemic caused by coronavirus disease 2019 (COVID-19) had alerted the population to the risk that small microorganisms can create for humankind’s wellbeing and survival. All of us have been affected, directly or indirectly, by this situation, and scientists all over the world have been trying to find solutions to fight this virus by killing it or by stop/decrease its spread rate. Numerous...
The Effectiveness of Therapeutic Vaccines for the Treatment of Cervical Intraep...
Ventura, Cathy; Luís, Ângelo; Soares, Christiane P.; Venuti, Aldo; Paolini, Francesca; Pereira, L.; Sousa, ÂngelaCervical cancer (CC) is a disease that affects many women worldwide, especially in low-income countries. The human papilloma virus (HPV) is the main causative agent of this disease, with the E6 and E7 oncoproteins being responsible for the development and maintenance of transformed status. In addition, HPV is also responsible for the appearance of cervical intraepithelial neoplasia (CIN), a pre-neoplastic condi...
In Silico Approaches: A Way to Unveil Novel Therapeutic Drugs for Cervical Canc...
Gomes, Diana; Silvestre, Samuel; Duarte, Ana Paula; Venuti, Aldo; Soares, Christiane P.; Passarinha, Luís; Sousa, ÂngelaCervical cancer (CC) is the fourth most common pathology in women worldwide and presents a high impact in developing countries due to limited financial resources as well as difficulties in monitoring and access to health services. Human papillomavirus (HPV) is the leading cause of CC, and despite the approval of prophylactic vaccines, there is no effective treatment for patients with pre-existing infections or ...
Minicircle DNA purification: Performance of chromatographic monoliths bearing l...
Almeida, Ana Margarida; Queiroz, João; Sousa, Fani; Sousa, ÂngelaMinicircle DNA (mcDNA) technology is in the vanguard of vectors designed for gene therapy, since the absence of prokaryotic sequences confers to mcDNA higher biosafety in comparison to other DNA vectors. However, the presence of other isoforms and non-recombined parental molecules hampers the isolation of supercoiled (sc) mcDNA with the chromatographic methods already established for plasmid purification. In th...
Cervical cancer and HPV infection: ongoing therapeutic research to counteract t...
Almeida, Ana Margarida; Queiroz, João; Sousa, Fani; Sousa, ÂngelaCervical cancer is the fourth most common cancer among women worldwide and its development is mainly associated with human papillomavirus infection, a highly sexually transmissible virus. The expression of E6 and E7 viral oncoproteins deregulates cell repairing mechanisms through impairment of tumor suppressor protein functions, such as p53 or retinoblastoma protein. Although the implementation of new preventiv...
Arginine homopeptides for plasmid DNA purification using monolithic supports
Cardoso, Sara; Sousa, Ângela; Queiroz, João; Azzoni, Adriano; Sousa, FaniPurification of plasmid DNA targeting therapeutic applications still presents many challenges, namely on supports and specific ligand development. Monolithic supports have emerged as interesting approaches for purifying pDNA due to its excellent mass transfer properties and higher binding capacity values. Moreover, arginine ligands were already described to establish specific and preferential interactions with ...
HPV-16 targeted DNA vaccine expression: The role of purification
Almeida, Ana Margarida; Tomás, Joana; Pereira, Patrícia; Queiroz, João; Sousa, Fani; Sousa, ÂngelaDNA vaccines have come to light in the last decades as an alternative method to prevent many infectious diseases, but they can also be used for the treatment of specific diseases, such as cervical cancer caused by Human Papillomavirus (HPV). This virus produces E6 and E7 oncoproteins, which alter the cell cycle regulation and can interfere with the DNA repairing system. These features can ultimately lead to the...
Chromatographic HPV-16 E6/E7 plasmid vaccine purification employing L-histidine...
Amorim, Lúcia; Gaspar, Rita; Pereira, Patrícia; Černigoj, Urh; Sousa, Fani; Queiroz, João; Sousa, ÂngelaAffinity chromatography based on amino acids as interacting ligands was already indicated as an alternative compared to ion exchange or hydrophobic interaction for plasmid DNA purification. Understanding the recognition mechanisms occurring between histidine-based ligands and nucleic acids enables more efficient purification of a DNA vaccine, as the binding and elution conditions can be adjusted in order to enh...