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Microwave hydrodiffusion and gravity: a green extraction technology for phenyle...

Sousa, Ana Sofia; Sousa, Sérgio; Silva, Inês V.; Reis, Celso A.; Coscueta, Ezequiel R.; Pintado, Maria Manuela

Watercress is a cruciferous vegetable and the primary natural source of phenylethyl isothiocyanate (PEITC), a powerful health-promoting agent. Conventional extraction of PEITC relies on harmful solvents, which limits sustainability. This study introduces a novel, solvent-free method—microwave hydrodiffusion and gravity (MHG)—to extract PEITC from watercress by-products (WCBP). Results showed that freezing WCBP ...


Impact of extracellular polymeric substances (EPS), biochar, and microbial inoc...

Overall, Alexandra; Moreira, Helena; Sousa, Ana Sofia; Wilfert, Philipp; van Loosdrecht, Mark; Castro, Paula M. L.; Pereira, S. I. A.


Application of a PGP bacterial bioinoculant in a vineyard: impact on soil bioch...

Prieto-Fernández, Ángeles; Pereira, Sofia; Sousa, Ana Sofia; Rodríguez-Garrido, Beatriz; González-Prieto, Serafin; Castro, Paula; Trasar-Cepeda, Carmen

Introduction: Sustainable agriculture increasingly relies on the use of environmentally friendly practices and biotechnological tools to maintain soil health and productivity. The ReCROP project, funded by the PRIMA-Med programme, aims to enhance soil functions and resilience through integrated cropping systems. As part of this initiative, we evaluated the effects of a plant growth-promoting (PGP) bacterial bio...


Exploring the bioactive potential of mushroom aqueous extracts: antimicrobial, ...

Afonso, Tiago Barros; Marçal, Sara; Vale, Pedro; Sousa, Ana Sofia; Nunes, João; Pintado, Manuela

Mushrooms provide essential nutrients and bioactive compounds, namely glucans, protein, and phenolic compounds. In this study, two aqueous extractions were performed (M1 and M2) using Pleurotus ostreatus, Pleurotus eryngii, and Agrocybe cylindracea. In M1, a hot extraction (extract M1) (90 °C, 700 rpm, 1 h) was performed. In M2, a room-temperature extraction (extract M2A) followed by a hot extraction (extract M...


Application of a PGP bacterial bioinoculant in a vineyard: impact on soil bioch...

Prieto-Fernández, Ángeles; Pereira, Sofia; Sousa, Ana Sofia; Rodríguez-Garrido, Beatriz; González-Prieto, Serafin; Castro, Paula; Trasar-Cepeda, Carmen

The ReCROP project, funded by the PRIMA-Med programme, focuses on the development of sustainable agricultural production systems through the combined use of biotechnological tools and environmentally friendly agronomic practices to enhance soil functions and health. As part of this project, a PGP bacterial strain was used as a bioinoculant in a vineyard of the Mencía variety, located within the DO Ribeiro regio...


Anthropometric and Body Composition Changes After Bariatric Surgery—The Effect ...

Guerra, Rita Soares; Pinho-Reis, Cíntia; Sousa, Ana Sofia; Mendes, Joana; Silva, Cláudia

The rise in obesity and its associated health problems increases the need for therapeutic approaches such as bariatric surgery. Therefore, this study aims to explore the changes in the anthropometric and body composition characteristics of patients undergoing bariatric surgery. A retrospective longitudinal study was conducted in subjects ≥ 18 y who underwent bariatric surgery and attended one nutrition appointm...

Date: 2025   |   Origin: IC-online


An overview on mushroom polysaccharides: health-promoting properties, prebiotic...

Araújo-Rodrigues, Helena; Sousa, Ana Sofia; Relvas, João Bettencourt; Tavaria, Freni K.; Pintado, Manuela

Mushroom polysaccharides are recognized as “biological response modifiers”. Besides several bioactivities, a growing interest in their prebiotic potential has been raised due to the gut microbiota modulation potential. This review comprehensively summarizes mushroom polysaccharides' biological properties, structure-function relationship, and underlying mechanisms. It provides a recent overview of the key findin...


Study of the impact of edible mushroom biomass obtained from by-products upcycl...

Cima, André; Voss, Glenise Bierhalz; Salsinha, Ana Sofia; Sousa, Ana Sofia; Gómez-García, Ricardo; Pintado, Manuela

In recent years, there has been a rise in the consumption of mushrooms, leading to an increase in the production of related by-products. Given their nutritional profile, mushrooms have the potential to represent a novel source of dietary fiber, which has been linked to prebiotic benefits. Through the utilization of a human feces’ fermentation model, this study aimed to assess the possible prebiotic effect of by...


Impact of in vitro gastrointestinal digestion on upcycled blackcurrant dried ex...

Sousa, Ana Sofia; Vilas-Boas, Ana A.; Gómez-García, Ricardo; Alonso, Mercedes; De Biasio, Filomena; Gorgoglione, Domenico; Fajardo, Paula

Pomaces, the major by-product of fruit juice processing industries, is rich in bioactive compounds. Among them, polyphenols boost the body's antioxidant capacity, improving cardiovascular health, reducing the risk of diabetes and inflammation, and promoting intestinal microbiota health. Blackcurrant (Ribes nigrum L.) is a highly antioxidant berry rich in anthocyanins, a polyphenols class that also gives the fru...


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