11 documents found, page 1 of 2

PREVALÊNCIA DA FIBROSE QUÍSTICA EM PORTUGAL: COMPARAÇÃO COM OUTROS PAÍSES DA EU...

RASTREIO NEONATAL DE HEMOGLOBINOPATIAS: IMPLEMENTAÇÃO E RESULTADOS PRELIMINARES

Gardnerella vaginalis outcompetes 29 other bacterial species isolated from BV p...

Despite the worldwide prevalence of bacterial vaginosis (BV), its etiology is still unknown. Although BV has been associated with the presence of biofilm, the ability of BV-associated bacteria to form biofilms is still largely unknown. Here, we isolated 30 BV-associated species and characterized their virulence, using an in vitro biofilm formation model. Our data suggests that Gardnerella vaginalis had the high...

Optimization of an experimental protocol to reduce gene expression variability ...

Analysis of gene expression variability in Staphylococcus epidermidis biofilms

Dissertação de mestrado em Molecular Genetics; S. epidermidis is the most frequently bacteria isolated from human epithelia, and for a long time, it has been regarded as an innocuous commensal bacterium. However, currently, this bacteria persist as a major cause of hospital and community-acquired infections. It is primarily associated with infections of indwelling medical devices, by the formation of a structur...

Assessing and reducing sources of gene expression variability in Staphylococcus...

Gene expression quantification can be a useful tool in studying the properties of bacterial biofilms. Unfortunately, techniques such as RNA extraction, cDNA synthesis, and quantitative PCR (qPCR) can introduce variability into mRNA transcript measurements, obscuring biologically relevant results. Here we sought to identify the steps that impair accurate gene expression quantification from Staphylococcus epiderm...

Can a specific sub-group of biofilm- forming Gardnerella vaginalis strains be t...

In the past half century, bacterial vaginosis (BV) has been a controversial topic in medical microbiology, and despite the wealth of information on this topic, the etiological agent has not yet been definitively identified [1]. The first advances on BV pointed Gardnerella vaginalis as the infectious causative agent of BV [2] but soon after it was found that G. vaginalis was also present in healthy women [3]. Ad...

Analysis of gene expression variability in Staphylococcus epidermidis biofilms

Staphylococcus epidermidis, a normal inhabitant of a human skin and mucosa, has emerged as one of the principle bacterial agents involved in nosocomial infections, particularly, in patients with indwelling medical devices. It’s pathogenesis is related with the ability to adhere and form biofilms on the surface of those medical devices and is also associated with patients’ immune system that can be compromised. ...

Gardnerella vaginalis virulence potential outcompetes with 30 other microorgani...

Bacterial vaginosis (BV) is the most common vaginal disorder affecting millions of women every year, and is usually associated with several adverse health outcomes, including preterm birth and acquisition of sexually transmitted diseases. However, the etiology of BV is still under debate. Recently, new fastidious anaerobic bacteria have been associated with BV, but there are very few studies that comprehensivel...

Functional analysis of virulence potential from Gardnerella vaginalis and other...

In the past half century, bacterial vaginosis (BV) has been a controversial topic in medical microbiology, and despite the wealth of information on this topic, the etiological agent has not yet been definitively identified [1]. The first advances on BV pointed Gardnerella vaginalis as the infectious causative agent of BV [2] but soon after it was found that G. vaginalis was also present in healthy women [3]. Ad...