15 documents found, page 1 of 2
Cumulus cell DNA damage linked to fertilization success in females with an ovul...
Rodrigues, B; Sousa, V; Esteves, F; Vale-Fernandes, E; Costa, S; Sousa, D; Brandão, R; Leal, C; Pires, J; Gaivão, I; Teixeira, JP; Nogueira, AJAIntracytoplasmic sperm injection (ICSI) is a widely used technique in fertility centers. ICSI success depends on both nuclear and cytoplasmic oocyte maturation. Cumulus cells, which surround the oocytes, play a pivotal role in oocyte competence. However, the significance of DNA damage in cumulus cells as a marker of fertilization success remains largely unexplored. This study aims to investigate the relationshi...
Equation-Oriented Modeling and Optimization of a Biorefinery Based on Avocado W...
Sousa, D; Rodrigues, D; Castro, PM; Matos, HADue to the growth in the consumption of avocado in recent years, the amount of waste caused by avocado peel and seed has increased. Avocado waste can be transformed into valuable products such as energy, biofuels, and biological products using integrated processes in a biorefinery. This paper considers the detailed modeling, simulation, and optimization of a biorefinery for the production of phenolic compounds,...
Different ability of multidrug-resistant and-sensitive counterpart cells to rel...
Sousa, D; Lima, RT; Lopes-Rodrigues, V; Gonzalez, E; Royo, F; Xavier, CPR; Falcón-Pérez, JM; Vasconcelos, MHCancer multidrug resistance (MDR) is one of the main challenges for cancer treatment efficacy. MDR is a phenomenon by which tumor cells become resistant to several unrelated drugs. Some studies have previously described the important role of extracellular vesicles (EVs) in the dissemination of a MDR phenotype. EVs’ cargo may include different players of MDR, such as microRNAS and drug-efflux pumps, which may be...
Acid sphingomyelinase deficiency: A clinical and immunological perspective
Pinto, C; Sousa, D; Ghilas, V; Dardis, A; Scarpa, M; Macedo, MFAcid sphingomyelinase deficiency (ASMD) is a lysosomal storage disease caused by deficient activity of acid sphingomyelinase (ASM) enzyme, leading to the accumulation of varying degrees of sphingomyelin. Lipid storage leads to foam cell infiltration in tissues, and clinical features including hepatosplenomegaly, pulmonary insufficiency and in some cases central nervous system involvement. ASM enzyme replacement...
Deep sequencing analysis reveals distinctive non-coding RNAs when comparing tum...
Sousa, D; Matthiesen, R; Lima, RT; Vasconcelos, MHMultidrug resistance (MDR) is one of the main limitations of cancer treatment. The overexpression of drug-eux pumps, such as P-glycoprotein (P-gp), is a major cause of MDR. Importantly, di erent studies have shown that extracellular vesicles (EVs) participate in the communication between MDR cells and drug-sensitive counterparts, promoting dissemination of the MDR phenotype. In the present work, we aimed to ide...
Direct Gaze Partially Overcomes Hemispatial Neglect and Captures Spatial Attention
Rato, M; Mares, I; Sousa, D; Senju, A; Martins, IDirect gaze has been shown to be a particularly important social cue, being preferentially processed even when unconsciously perceived. Results from several visual search tasks further suggest that direct gaze modulates attention, showing a faster orientation to faces perceived as looking toward us. The present study aimed to analyze putative modulation of spatial attention by eye gaze direction in patients wit...
Synthesis and evaluation of the tumor cell growth inhibitory potential of new p...
Bizarro, A; Sousa, D; Lima, RT; Musso, L; Cincinelli, R; Zuco, V; Cesare, M; Dallavalle, S; Vasconcelos, MHBackground: Heat shock protein 90 (HSP90) is a well-known target for cancer therapy. In a previous work, some of us have reported a series of 3-aryl-naphtho[2,3-d]isoxazole-4,9-diones as inhibitors of HSP90. Methods: In the present work, various compounds with new chromenopyridinone and thiochromenopyridinone scaffolds were synthesized as potential HSP90 inhibitors. Their binding affinity to HSP90 was studied i...
Novel monoclonal antibody L2A5 specifically targeting sialyl-Tn and short glyca...
Loureiro, L; Sousa, D; Ferreira, D; Chai, W; Lima, L; Pereira, C; Lopes, C; Correia, V; Silva, L; Li, C; Santos, L; Ferreira, JA; Barbas, A; Palma, AIncomplete O-glycosylation is a feature associated with malignancy resulting in the expression of truncated glycans such as the sialyl-Tn (STn) antigen. Despite all the progress in the development of potential anti-cancer antibodies, their application is frequently hindered by low specificities and cross-reactivity. In this study, a novel anti-STn monoclonal antibody named L2A5 was developed by hybridoma techno...
Cell surface targeting of the Melanocortin 5 Receptor (MC5R) requires serine-ri...
Rodrigues, AR; Sousa, D; Almeida, H; Gouveia, AlexandraThe Melanocortin 5 Receptor (MC5R) is a cell surface receptor that belongs to the class of G-protein coupled receptors (GPCRs), which comprises an intracellular carboxylic domain, seven transmembrane helices and an extracellular amino terminal. Over the last few years, MC5R has been implicated in the regulation of lipid metabolism in exocrine glands, muscle and even in adipose tissue and its function is quite d...
Cerebral venous thrombosis causing posterior fossa lesions: description of a ca...
Sousa, D; Ferro, J; Canhão, P; Barinagarrementeria, F; Bousser, MG; Stam, J; Pinto, A; Baptista, M; Béjot, Y; Dequatre-Poncelle, NBACKGROUND: Isolated posterior fossa parenchymal lesions associated with cerebral venous thrombosis (CVT) are rare. Posterior fossa lesions are an independent predictor of death in CVT. We aim to describe the characteristics and outcome of patients with CVT and isolated posterior fossa lesions and assess the safety of anticoagulation in patients with posterior fossa lesions associated with CVT. METHODS: We retr...