7 documents found, page 1 of 1
Serum amyloid A proteins reduce bone mass during mycobacterial infections
Gomes, AC; Sousa, DM; Oliveira, TC; Fonseca, O; Pinto, RJ; Silvério, D; Fernandes, AI; Moreira, AC; Silva, T; Teles, MJ; Pereira, L; Saraiva, MIntroductionOsteopenia has been associated to several inflammatory conditions, including mycobacterial infections. How mycobacteria cause bone loss remains elusive, but direct bone infection may not be required. MethodsGenetically engineered mice and morphometric, transcriptomic, and functional analyses were used. Additionally, inflammatory mediators and bone turnover markers were measured in the serum of healt...
Polymeric microspheres/cells/extracellular matrix constructs produced by auto-a...
Mielan, B; Sousa, DM; Krok-Borkowicz, M; Eloy, P; Dupont, C; Lamghari, M; Pamula, EModular tissue engineering (MTE) is a novel “bottom-up” approach to create engineered biological tissues from microscale repeating units. Our aim was to obtain microtissue constructs, based on polymer microspheres (MSs) populated with cells, which can be further assembled into larger tissue blocks and used in bone MTE. Poly(L-lactide-co-glycolide) MS of 165 ± 47 µm in diameter were produced by oil-in-water emul...
Sympathetic activity in breast cancer and metastasis: partners in crime
Conceição, F; Sousa, DM; Paredes, J; Lamghari, MThe vast majority of patients with advanced breast cancer present skeletal complications that severely compromise their quality of life. Breast cancer cells are characterized by a strong tropism to the bone niche. After engraftment and colonization of bone, breast cancer cells interact with native bone cells to hinder the normal bone remodeling process and establish an osteolytic “metastatic vicious cycle”. The...
Nociceptive mechanisms driving pain in a post-traumatic osteoarthritis mouse model
Alves, CJ; Couto, M; Sousa, DM; Magalhães, A; Neto, E; Leitão, L; Conceição, F; Monteiro, AC; Ribeiro-da-Silva, M; Lamghari, MIn osteoarthritis (OA), pain is the dominant clinical symptom, yet the therapeutic approaches remain inadequate. The knowledge of the nociceptive mechanisms in OA, which will allow to develop effective therapies for OA pain, is of utmost need. In this study, we investigated the nociceptive mechanisms involved in post-traumatic OA pain, using the destabilization of the medial meniscus (DMM) mouse model. Our resu...
Exploring Poly(Ethylene Glycol)-Poly(Trimethylene Carbonate) Nanoparticles as C...
Leite, DM; Sousa, DM; Lamghari, M; Pêgo, APCurrent treatment options for bone-related disorders rely on a systemic administration of therapeutic agents that possess low solubility and intracellular bioavailability, as well as a high pharmacokinetic variability, which in turn lead to major off-target side effects. Hence, there is an unmet need of developing drug delivery systems that can improve the clinical efficacy of such therapeutic agents. Nanoparti...
The Mechanisms Underlying the Biological Response to Wear Debris in Periprosthe...
Couto, M; Vasconcelos, DP; Sousa, DM; Sousa, B; Conceição, F; Neto, E; Lamghari, M; Alves, CJJoint replacement surgery is the gold-standard therapeutic approach to treat patients with end-stage hip and knee arthritis, providing pain relief and joint function recovery. Despite the improvements in implant design and surgical techniques, revisions after total joint replacement are expected to grow. The periprosthetic inflammation, featured by the sustained inflammatory response to the implant debris, elic...
Osteoblasts are inherently programmed to repel sensory innervation
Leitão, L; Neto, E; Conceição, F; Monteiro, AC; Couto, M; Alves, CJ; Sousa, DM; Lamghari, MTissue innervation is a complex process controlled by the expression profile of signaling molecules secreted by tissue-resident cells that dictate the growth and guidance of axons. Sensory innervation is part of the neuronal network of the bone tissue with a defined spatiotemporal occurrence during bone development. Yet, the current understanding of the mechanisms regulating the map of sensory innervation in th...