49 documents found, page 1 of 5
The role of ethanol oxidation during carboxydotrophic growth of clostridium aut...
Diender, Martijn; Dykstra, James C.; Parera Olm, Ivette; Kengen, Servé W. M.; Stams, Alfons Johannes Maria; Sousa, Diana Zita MachadoThe WoodLjungdahl pathway is an ancient metabolic route used by acetogenic carboxydotrophs to convert CO into acetate, and some cases ethanol. When produced, ethanol is generally seen as an end product of acetogenic metabolism, but here we show that it acts as an important intermediate and co-substrate during carboxydotrophic growth of Clostridium autoethanogenum. Depending on CO availability, C. autoethanogenu...
Principles, advances, and perspectives of anaerobic digestion of lipids
Holohan, B. Conall; Duarte, Maria Salomé; Szabo-Corbacho, Alejandra; Cavaleiro, A. J.; Salvador, Andreia F.; Pereira, M. A.; Ziels, Ryan M.Several problems associated with the presence of lipids in wastewater treatment plants are usually overcome by removing them ahead of the biological treatment. However, because of their high energy content, waste lipids are interesting yet challenging pollutants in anaerobic wastewater treatment and codigestion processes. The maximal amount of waste lipids that can be sustainably accommodated, and effectively c...
Enhanced glycerol conversion by Thermoanaerobacter strains
Magalhães, Carla Isabel Pereira; Ribeiro, Joaquim Alfredo França Martins; Guedes, Ana P.; Arantes, Ana Luísa; Sousa, Diana Zita MachadoGlycerol-rich waste streams produced as a surplus by the biodiesel industry can be treated and valorized by anaerobic microbial communities to produce biogas. Glycerol is a highly reduced compound. Its complete degradation to methane and carbon dioxide requires a syntrophic cooperation of anaerobic bacteria and archaea, either directly or through propionate, lactate or ethanol as intermediates. The aim of this ...
Acetate degradation at low pH by the moderately acidophilic sulfate reducer Aci...
Sánchez-Andrea, Irene; van der Graaf, Charlotte M.; Hornung, Bastian; Bale, Nicole J.; Jarzembowska, Monika; Sousa, Diana Zita MachadoIn acid drainage environments, biosulfidogenesis by sulfate-reducing bacteria (SRB) attenuates the extreme conditions by enabling the precipitation of metals as their sulfides, and the neutralization of acidity through proton consumption. So far, only a handful of moderately acidophilic SRB species have been described, most of which are merely acidotolerant. Here, a novel species within a novel genus of moderat...
Anaerobic microbial methanol conversion in marine sediments
Fischer, Peter Q.; Sánchez-Andrea, Irene; Stams, Alfons Johannes Maria; Villanueva, Laura; Sousa, Diana Zita MachadoMethanol is an ubiquitous compound that plays a role in microbial processes as a carbon and energy source, intermediate in metabolic processes or as end product in fermentation. In anoxic environments, methanol can act as sole carbon and energy source for several guilds of microorganisms: sulfate reducing microorganisms, nitrate reducing microorganisms, acetogens and methanogens. In marine sediments, these guil...
Propionate production from carbon monoxide by synthetic cocultures of acetobact...
Moreira, João Paulo Carvalho; Diender, M.; Arantes, Ana Luísa; Boeren, S.; Stams, Alfons Johannes Maria; Alves, M. M.; Alves, Joana I.Gas fermentation is a promising way to convert CO-rich gases to chemicals. We studied the use of synthetic cocultures composed of carboxydotrophic and propionigenic bacteria to convert CO to propionate. So far, isolated carboxydotrophs cannot directly ferment CO to propionate, and therefore, this cocultivation approach was investigated. Four distinct synthetic cocultures were constructed, consisting of Acetobac...
Effect of sulfate on carbon monoxide conversion by a thermophilic syngas-fermen...
Alves, J. I.; Visser, Michael; Arantes, Ana Luísa; Nijsse, Bart; Plugge, Caroline M.; Alves, M. M.; Stams, Alfons Johannes MariaA syngas-degrading enrichment culture, culture T-Syn, was dominated by a bacterium closely related to Desulfofundulus australicus strain AB33T (98% 16S rRNA gene sequence identity). Culture T-Syn could convert high CO concentrations (from pCO 34 kPa to pCO 170 kPa), both in the absence and in the presence of sulfate as external electron acceptor. The products formed from CO conversion were H2 and acetate. With ...
Genome-guided analysis allows the identification of novel physiological traits ...
Strepis, Nikolaos; Naranjo, Henry D.; Meier-Kolthoff, Jan; Göker, Markus; Shapiro, Nicole; Kyrpides, Nikos; Klenk, Hans-Peter; Schaap, Peter J.The genus Trichococcus currently contains nine species: T. flocculiformis, T. pasteurii, T. palustris, T. collinsii, T. patagoniensis, T. ilyis, T. paludicola, T. alkaliphilus, and T. shcherbakoviae. In general, Trichococcus species can degrade a wide range of carbohydrates. However, only T. pasteurii and a non-characterized strain of Trichococcus, strain ES5, have the capacity of converting glycerol to mainly ...
The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulf...
Sánchez-Andrea, Irene; Guedes, Iame Alves; Hornung, Bastian; Boeren, Sjef; Lawson, Christopher E.; Sousa, Diana Zita Machado; Bar-Even, ArrenSix CO2 fixation pathways are known to operate in photoautotrophic and chemoautotrophic microorganisms. Here, we describe chemolithoautotrophic growth of the sulphate-reducing bacterium Desulfovibrio desulfuricans (strain G11) with hydrogen and sulphate as energy substrates. Genomic, transcriptomic, proteomic and metabolomic analyses reveal that D. desulfuricans assimilates CO2 via the reductive glycine pathway...
Long-chain fatty acids degradation by desulfomonile species and proposal of can...
Alves, J. I.; Salvador, Andreia Filipa Ferreira; Carvalho, Ana Rita Castro; Zheng, Ying; Nijsse, Bart; Atashgahi, Siavash; Sousa, Diana Zita MachadoMicrobial communities with the ability to convert long-chain fatty acids (LCFA) coupled to sulfate reduction can be important in the removal of these compounds from wastewater. In this work, an enrichment culture, able to oxidize the long-chain fatty acid palmitate (C16:0) coupled to sulfate reduction, was obtained from anaerobic granular sludge. Microscopic analysis of this culture, designated HP culture, reve...