61 documents found, page 1 of 7
A new approach for extracellular RNA recovery from Rhodovulum sulfidophilum
Riscado, Micaela; Carapito, Rita; Maia, Cláudio J.; Pichon, Chantal; Freire, Mara G.; Sponchioni, Mattia; Sousa, FaniThe development of RNA-based drugs is highly pursued due to the possibility of creating viable and effective therapies. However, their translation to clinical practice strongly depends on efficient technologies to produce substantial levels of these biomolecules, with high purity and high quality. RNAs are commonly produced by chemical or enzymatic methods, displaying these limitations. In this sense, recombina...
Effective separation of nucleic acids from complex bacterial lysates using thre...
Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, FaniNo abstract available.
Multimodal ionic liquid-based chromatographic supports for an effective RNA pur...
Carapito, Rita; Bernardo, Sandra C.; Pereira, Matheus M.; Neves, Márcia C.; Freire, Mara G.; Sousa, FaniNucleic acids have been considered interesting molecules to be used as biopharmaceuticals for the treatment of various diseases, in gene therapy strategies. In particular, RNA arises as the most promising approach because it does not require access to the nucleus of cells to exert its function; however, it is quite challenging due to its labile nature. To increase the possibility of translating RNA-based techno...
Purification of RNA from recombinant lysates using biocompatible amino-acid-bas...
Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, FaniRNA-based biopharmaceuticals are being envisioned as a powerful tool for the development of innovative medicines for prevalent diseases. Despite their undeniable relevance, the ubiquitous unstable nature of RNA coupled with the laborious and costly methods required for its extraction and purification still challenge the widespread application of this biopolymer. On the basis of the high affinity between amino-a...
Aqueous biphasic systems composed by biocompatible amino acid-based ionic liqui...
Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, FaniThe emergence of RNA as a promising biopharmaceutical paved the way for the development of innovative medicines with broad therapeutic and prophylactic efficiencies in infectious diseases and cancer. Notwithstanding their relevance on this field, the labile nature and intrinsic low stability of RNA coupled with the laborious and costly methods of extraction and purification of this biopolymer constitute the mai...
Integrated extraction, purification and preservation of DNA with ionic liquid-b...
Valente, Ana I.; Dinis, Teresa B. V.; Tavares, Ana P. M.; Sousa, Fani; Freire, Mara G.The production of deoxyribonucleic acid (DNA) in large-scale for therapeutic purposes presents several challenges. An effective downstream process is highly demanding, as it should be capable of extracting, purifying, and preserving DNA integrity, by reducing its degradation by endonucleases.1,2 A technique that allows the integration of several downstream steps is aqueous biphasic systems (ABS). Through the al...
Optimization of the production and purification of non-viral vectors for gene t...
Valente, Ana I.; Castro, Leonor S.; Rios, Inês D.; Pedro, Augusto Q.; Tavares, Ana P. M.; Sousa, Fani; Freire, Mara G.Cancer ranks the second leading cause of death globally and has significant societal and economic costs. To address this issue, the production of biopharmaceuticals is standing out. Nucleic acids are gaining popularity in preventing infections and as therapeutic agents in gene therapy, aiming to fix the target dysfunction by transfecting eukaryotic cells with gene-based products. Non-viral vectors, including pl...
Multimodal ionic liquid-based chromatographic supports for an effective RNA pur...
Carapito, Rita; Bernardo, Sandra C.; Pereira, Matheus M.; Neves, Márcia C.; Freire, Mara G.; Sousa, FaniNucleic acids have been considered interesting molecules to be used as biopharmaceuticals for the treatment of various diseases, in gene therapy strategies. In particular, RNA arises as the most promising approach because it does not require access to the nucleus of cells to exert its function; however, it is quite challenging due to its labile nature. To increase the possibility of translating RNA-based techno...
Integrated platform resorting to ionic liquids comprising the extraction, purif...
Dinis, Teresa B. V.; Valente, Ana I.; Tavares, Ana P. M.; Sousa, Fani; Freire, Mara G.The large-scale production of therapeutically targeted-deoxyribonucleic acid (DNA) has passed through several challenges, postponing the tangible implementation of an effective, economic and sustainable manufacturing system. Such challenges comprise the need to develop an integrative downstream process able to extract, purify and long-term preserve DNA, whilst reducing the risk of degradation by endonucleases t...
Multimodal ionic liquid-based chromatographic supports for an effective RNA pur...
Carapito, Ana Rita Mugeiro; Bernardo, Sandra C.; Pereira, Matheus M.; Neves, Márcia C.; Freire, Mara G.; Sousa, FaniNucleic acids have been considered interesting molecules to be used as biopharmaceuticals for the treatment of various diseases, in gene therapy strategies. In particular, RNA arises as the most promising approach because it does not require access to the nucleus of cells to exert its function; however, it is quite challenging due to its labile nature. To increase the possibility of translating RNA-based techno...