19 documents found, page 1 of 2
Adaptation of key bacterial vaginosis-associated bacteria to a medium simulatin...
Sousa, Lúcia Filipa Guimarães Vieira; França, Ângela Maria Oliveira Sousa; Pinheiro, Vânia Oliveira; Muzny, Christina A.; Cerca, NunoTo study the influence of simulated in vivo conditions on the interactions of key BVAB, we analyzed the transcriptome of a triple-species biofilm composed of G. vaginalis, F. vaginae, and P. bivia, when biofilms were grown in a rich medium versus a medium mimicking genital tract secretions. Triple-species biofilms formed in mGTS were also analyzed by qPCR to determine each species percentage, revealing that the...
Key bacterial vaginosis-associated bacteria influence each others growth in bio...
Sousa, Lúcia Filipa Guimarães Vieira; Muzny, Christina A.; Cerca, NunoBacterial vaginosis (BV) is a very common gynaecologic condition affecting women of reproductive age worldwide. BV is characterized by a depletion of lactic acid-producing Lactobacillus species and an increase in strict and facultative anaerobic bacteria that develop a polymicrobial biofilm on the vaginal epithelium. Despite multiple decades over research, the etiology of this infection is still not clear. Howe...
Unveiling microbial interactions between Gardnerella vaginalis, Fannyhessea vag...
Sousa, Lúcia Filipa Guimarães VieiraA vaginose bacteriana (VB) é uma infeção vaginal comum em mulheres em idade reprodutiva, caracterizada por um desequilíbrio na microbiota vaginal com um decréscimo do número de espécies de Lactobacillus e um crescimento excessivo de bactérias anaeróbias estritas e facultativas. Apesar de vários estudos demonstrarem uma elevada diversidade de espécies detetadas em casos de VB, a sua etiologia ainda não é clara. ...
Microbial interactions among Gardnerella, Prevotella and Fannyhessea prior to i...
Muzny, Christina A.; Elnaggar, Jacob H.; Sousa, Lúcia Filipa Guimarães Vieira; Lima, Ângela Martins; Aaron, Kristal J.; Eastlund, Isaac C.Introduction The aetiology of bacterial vaginosis (BV), a biofilm-associated vaginal infection, remains unknown. Epidemiologic data suggest that it is sexually transmitted. BV is characterised by loss of lactic acid-producing lactobacilli and an increase in facultative and strict anaerobic bacteria. Gardnerella spp are present in 95\%\textendash100\% of cases; Gardnerella vaginalis has been found to be more vir...
Explorando os mistérios da vaginose bacteriana: como as espécies microbianas se...
Sousa, Lúcia Filipa Guimarães Vieira; França, Ângela Maria Oliveira Sousa; Muzny, Christina A.; Cerca, NunoA vaginose bacteriana (VB) é a infeção vaginal mais comum em mulheres em todo o mundo. A VB é caracterizada por um desequilíbrio na microbiota vaginal marcado por uma diminuição do número de espécies de Lactobacillus e um aumento do número de bactérias anaeróbias, que crescem num biofilme polimicrobiano. A formação de um biofilme polimicrobiano em casos de VB é uma das características mais associadas à VB, no e...
Gardnerella vaginalis, Fannyhessea vaginae, and Prevotella bivia strongly influ...
Sousa, Lúcia Filipa Guimarães Vieira; Novak, Juliano; França, Ângela Maria Oliveira Sousa; Muzny, Christina A.; Cerca, NunoBacterial vaginosis (BV), the most common vaginal infection worldwide, is characterized by the development of a polymicrobial biofilm on the vaginal epithelium. While Gardnerella spp. have been shown to have a prominent role in BV, little is known regarding how other species can influence BV development. Thus, we aimed to study the transcriptome of Gardnerella vaginalis, Fannyhessea vaginae, and Prevotella bivi...
Longitudinal analysis of select bacterial species composition among women with ...
George, S.; Dong, C.; Aaron, K.; Sherman, A.; Van Gerwen, O.; Tamhane, A.; Taylor, C.; Sousa, Lúcia Filipa Guimarães Vieira; Almeida, C.; Cerca, NunoObjective Key BV-associated bacteria that may be important in incident BV (iBV) pathogenesis are Gardnerella species and Prevotella bivia. We quantified the amount of these bacterial species in clue cell biofilms in longitudinal vaginal specimens from iBV cases and controls using peptide nucleic acid fluorescence in situ hybridization (PNA-FISH). Study Design Women with optimal vaginal microbiota self-collected...
The role of prevotella species in female genital tract infections
George, Sheridan D.; Van Gerwen, Olivia T.; Dong, Chaoling; Sousa, Lúcia Filipa Guimarães Vieira; Cerca, Nuno; Elnaggar, Jacob H.Female genital tract infections (FGTIs) include vaginal infections (e.g., bacterial vaginosis [BV]), endometritis, pelvic inflammatory disease [PID], and chorioamnionitis [amniotic fluid infection]. They commonly occur in women of reproductive age and are strongly associated with multiple adverse health outcomes including increased risk of HIV/sexually transmitted infection acquisition and transmission, inferti...
Fighting polymicrobial biofilms in bacterial vaginosis
Sousa, Lúcia Filipa Guimarães Vieira; Pereira, Sofia A.; Cerca, NunoBacterial vaginosis (BV) is the most common cause of vaginal discharge and is often associated with other health consequences mainly in pregnant women. BV is described by an imbalance in the vaginal microbiota where strictly and facultative anaerobic bacteria outgrow the lactic acid- and hydrogen peroxide-producing Lactobacillus species. The species involved in BV are capable to grow and form a polymicrobial bi...
Vaginal sheets with Thymbra capitata essential oil for the treatment of bacteri...
Tomás, Mariana; Sousa, Lúcia Filipa Guimarães Vieira; Oliveira, Ana Sofia; Gomes, Carolina P.; Palmeira-de-Oliveira, Ana; Cavaleiro, CarlosWe aimed to incorporate Thymbra capitata essential oil (TCEO), a potent antimicrobial natural product against bacterial vaginosis (BV)-related bacteria, in a suitable drug delivery system. We used vaginal sheets as dosage form to promote immediate relief of the typical abundant vaginal discharge with unpleasant odour. Excipients were selected to promote the healthy vaginal environment reestablishment and bioadh...