282 documents found, page 1 of 29
Symmetrical Neck Swelling After Percutaneous Coronary Intervention
Pereira Dias, Liliana; Coelho, Ana Cristina; Mota, Cristina; Sousa, Maria JoãoCervical edema after iodinated contrast media administration can raise the suspicion of a hypersensitivity reaction, but other diagnoses must be considered. The authors present a rare case of a man who, during the diagnostic investigation of a patient with thoracic pain developed severe submandibular swelling and local pain after a percutaneous coronary intervention with iodinated contrast media. The cervical c...
Current and novel approaches in yeast cell death research
Chaves, Susana Alexandra Rodrigues; Rego, António; Cátia S. Pereira; Sousa, Maria João; Côrte-Real, ManuelaThe study of cell death mechanisms in fungi, particularly yeasts, has gained substantial interest in recent decades driven by the potential for biotechnological advancements and therapeutic interventions. Examples include the development of robust yeast strains for industrial fermentations and high-value compound production, novel food preservation strategies against spoilage yeasts, and the identification of t...
Microbial production of single cell oil from biowaste-derived volatile fatty ac...
Fernandes, Ticiana; Parpot, Pier; Soares-Silva, Isabel; Sousa, Maria João; Franco-Duarte, RicardoEfforts to achieve a circular economy emphasize waste minimization while maximizing its value through recycling and reuse. Volatile Fatty Acids (VFAs), derived from organic waste streams, hold biotechnological potential as raw materials for producing value-added compounds offering sustainable alternatives to traditional petroleum-based approaches. The phenotypic and genomic plasticity of non-Saccharomyces yeast...
Bee Venom-Loaded Niosomes as Innovative Platforms for Cancer Treatment: Develop...
Pinto, Maria Beatriz; Pires, Patrícia C.; Calhelha, Ricardo C.; Silva, Ana Rita; Sousa, Maria João; Vilas-Boas, Miguel; Falcão, Soraia; Veiga, FranciscoDespite past efforts towards therapeutical innovation, cancer remains a highly incident and lethal disease, with current treatments lacking efficiency and leading to severe side effects. Hence, it is imperative to develop new, more efficient, and safer therapies. Bee venom has proven to have multiple and synergistic bioactivities, including antitumor effects. Nevertheless, some toxic effects have been associate...
Combining fluconazole with benzo[a]phenoxazine derivatives as a promising strat...
Pacheco, Maria Inês Costa; Guimarães, Bárbara; Pereira-Silva, Patrícia; Costa-Barbosa, Augusto; Gonçalves, M. Sameiro T.; Sousa, Maria JoãoThe rise in non-albicans Candida species, exhibiting unpredictable antifungal resistance, complicates treatment and contributes to the growing threat of invasive, life-threatening infections. This study evaluates the antifungal activity of four benzo[a]phenoxazine derivatives (C34, C35, A42, and A44) against 14 Candida strains following EUCAST standards. Fluconazole interactions are analysed through fractional ...
The fungicide cymoxanil impairs respiration in Saccharomyces cerevisiae via cyt...
Mendes, Filipa Diana Silva; Santos-Pereira, Cátia; Vieira, Tatiana F.; Pinto, Mélanie Martins; Castro, Bruno B.; Sousa, Sérgio F.; Sousa, Maria JoãoCymoxanil (CYM) is a widely used synthetic acetamide fungicide, but its biochemical mode of action remains elusive. Since CYM inhibits cell growth, biomass production, and respiration in Saccharomyces cerevisiae, we used this model to characterize the effect of CYM on mitochondria. We found it inhibits oxygen consumption in both whole cells and isolated mitochondria, specifically inhibiting cytochrome c oxidase...
Synthesis, photophysics, and potential antifungal activity of Benzo[a]phenoxazines
Guimarães, Bárbara S. G.; Sousa, Maria João; Gonçalves, M. Sameiro T.The synthesis and full characterization of new hydroxyl benzo[a]phenoxazinium chlorides with N-(di)propyl and/or N-isopropyl groups in the 5 and 9 positions are described. Photophysical studies carried out in ethanol and water revealed strong absorbances and significant fluorescence emissions at up to 676 nm. The antifungal activity was assessed against Saccharomyces cerevisiae and the results provide possible ...
Novel yeast-based biosensor for environmental monitoring of tebuconazole
Mendes, Filipa; Miranda, Eduarda; Amaral, Leslie Ann Silva; Carvalho, Carla; Castro, Bruno B.; Sousa, Maria João; Chaves, Susana Alexandra RodriguesAbstract: Due to increasing demand for high and stable crop production, human populations are highly dependent on pesticide use for growing and storing food. Environmental monitoring of these agrochemicals is therefore of utmost importance, because of their collateral effects on ecosystem and human health. Even though most current-use analytical methods achieve low detection limits, they require procedures that...
The Digital Transition of Healthcare: A Pilot Experience
Amorim, Liliana; Moaris, Joana; Costa, Rui; Castanho, Teresa; Neves, Carolina; Sousa, Maria João; Sousa, Rita; Martins, Filipa; Domingues, RuiThe healthcare context is undergoing a significant transformation, facing a series of complex and unprecedented challenges. This transformation is triggered by accessibility constraints to healthcare services, increased expectations from patients and sing financial burdens. Rather than being seen as unsolvable obstacles, these challenges can be perceived as powerful stimuli, encouraging the search for innovativ...
Anticancer activity of benzo[a]phenoxazine compounds promoting lysosomal dysfun...
Ferreira, João Carlos Canossa; Gonçalves, M. Sameiro T.; Preto, Ana; Sousa, Maria JoãoSpecific cancer therapy remains a problem to be solved. Breast and colorectal cancer are among the cancers with the highest prevalence and mortality rates. Although there are some therapeutic options, there are still few effective agents for those cancers, which constitutes a clinical problem that requires further research efforts. Lysosomes play an important role in cancer cells’ survival, and targeting lysoso...