267 documents found, page 1 of 27
Novel yeast-based biosensor for environmental monitoring of tebuconazole
Mendes, Filipa; Miranda, Eduarda; Amaral, Leslie Ann Silva; Carvalho, Carla; Castro, Bruno B.; Sousa, Maria João; Chaves, Susana Alexandra RodriguesAbstract: Due to increasing demand for high and stable crop production, human populations are highly dependent on pesticide use for growing and storing food. Environmental monitoring of these agrochemicals is therefore of utmost importance, because of their collateral effects on ecosystem and human health. Even though most current-use analytical methods achieve low detection limits, they require procedures that...
Targeting lysosomes in colorectal cancer: exploring the anticancer activity of ...
Ferreira, João C. C.; Granja, Sara; Almeida, Ana F.; Baltazar, Fátima; Gonçalves, M. Sameiro T.; Preto, Ana; Sousa, Maria JoãoColorectal cancer (CRC) has been ranked as one of the cancer types with a higher incidence and one of the most mortal. There are limited therapies available for CRC, which urges the finding of intracellular targets and the discovery of new drugs for innovative therapeutic approaches. In addition to the limited number of effective anticancer agents approved for use in humans, CRC resistance and secondary effects...
Contributions of adaptive laboratory evolution towards the enhancement of the b...
Fernandes, Ticiana; Osório, Carolina; Sousa, Maria João; Franco-Duarte, RicardoChanges in biological properties over several generations, induced by controlling shortterm evolutionary processes in the laboratory through selective pressure, and whole-genome re-sequencing, help determine the genetic basis of microorganism’s adaptive laboratory evolution (ALE). Due to the versatility of this technique and the imminent urgency for alternatives to petroleum-based strategies, ALE has been activ...
Targeting lysosomes in colorectal cancer: exploring the anticancer activity of ...
Ferreira, João C. C.; Granja, Sara Costa; Almeida, Ana F.; Baltazar, Fátima; Gonçalves, M. Sameiro T.; Preto, Ana; Sousa, Maria JoãoColorectal cancer (CRC) has been ranked as one of the cancer types with a higher incidence and one of the most mortal. There are limited therapies available for CRC, which urges the finding of intracellular targets and the discovery of new drugs for innovative therapeutic approaches. In addition to the limited number of effective anticancer agents approved for use in humans, CRC resistance and secondary effects...
Evaluation of antifungal activity and potential application as fluorescent prob...
Gomes, Vanessa S. D.; Ferreira, João C. C.; Boto, Renato E.; Almeida, Paulo; Fernandes, José R.; Sousa, Maria João; Reis, Lucinda V.The antifungal performance and the possible use as fluorescent probes of a series of squarylium dyes derived from indolenine and benzo[e]indole previously synthesized was evaluated. Some photophysical properties were performed in ethanol and phosphate buffer, and the type of aggregates form in phosphate buffer was analyzed. Using the 1,3-diphenylisobenzofuran assay, a qualitative assessment of the capacity of d...
Novel benzo[α]phenoxazinium chlorides functionalized with sulfonamide groups as...
Ferreira, J. C. C.; Sousa, Rui Pedro Carvalho Lima; Preto, Ana; Sousa, Maria João; Gonçalves, M. Sameiro T.The demand for new fluorophores for different biological target imaging is increasing. Benzo[<i>a</i>]phenoxazine derivatives are fluorochromophores that show promising optical properties for bioimaging, namely fluorescent emission at the NIR of the visible region, where biological samples have minimal fluorescence emission. In this study, six new benzo[<i>a</i>]phenoxazinium chlorides possessing sulfonamide gr...
A glimpse at an early stage of microbe domestication revealed in the variable g...
Silva, Margarida; Pontes, Ana; Franco-Duarte, Ricardo; Soares, Pedro; Sampaio, José Paulo; Sousa, Maria João; Brito, Patrícia HMicrobe domestication has a major applied relevance but is still poorly understood from an evolutionary perspective. The yeast Torulaspora delbrueckii is gaining importance for biotechnology but little is known about its population structure, variation in gene content or possible domestication routes. Here, we show that T. delbrueckii is composed of five major clades. Among the three European clades, a lineage ...
Cymoxanil inhibits respiration through inhibition of mitochondrial complex IV
Mendes, Filipa; Vieira, Tatiana F.; Pereira, Cátia Sofia Santos; Pinto, Mélanie Martins; Castro, Bruno B.; Sousa, Sérgio F.; Sousa, Maria JoãoCymoxanil is a synthetic acetamide fungicide, used against oomycetes. It was first introduced in 1977 and can be used against downy mildew diseases induced by Plasmopara viticola in grapevine cultures and late blight diseases caused by Phytophthora infestans, in tomatoes and potatoes cultures. This fungicide is used in mixed formulations and its higher solubility enables a relatively widespread occurrence in to...
Hop by-products: pharmacological activities and potential application as cosmetics
Pereira, Olívia R.; Santos, Gleiciara; Sousa, Maria JoãoHops (Humulus lupulus L.) are known worldwide as a raw material in beer production due their flavor and preservative values. The beneficial properties of the plant have been mostly associated with the female hop inflorescences (cones), which is also the part used in the brewing industry. However, some studies indicate the presence of compounds associated with health benefits in the vegetative parts of hops or s...
Análise preliminar de formulações Ayurvédicas como fonte potencial para cosméticos
Zacharias, Alessandra; Martins, Valter; Paula, Vanessa B.; Morais, Jorge Sá; Sousa, Maria JoãoGhee (manteiga clarificada) é uma preparação Ayurvédica (medicina tradicional indiana) rica em PUFA’s, com potencial anti-inflamatório e antioxidante. Além do uso culinário, é usado em queimaduras, cicatrização e tratamento de doenças cutâneas. Rosmarinus officinalis L. e Rosa spp. apresentam atividades antibacteriana, anti-inflamatória, anti tumoral e antioxidante. Desde a antiguidade, estas plantas são utiliz...