22 documents found, page 1 of 3
Understanding lactoferrin multifunctionality by analyzing the full spectrum of ...
Ferreira, Sofia; Guedes, Joana P.; Rocha, Juliana F.; Sousa, Sérgio F.; Rodrigues, L. R.; Côrte-Real, Manuela; Soares, Pedro; Cátia S. PereiraLactoferrin (LF) is a multifunctional protein involved in critical biological processes such as immune response, antimicrobial activity, and iron regulation [1]. Its diverse roles include inhibiting inflammation, infections, and carcinogenesis [2], as well as modulating cellular processes by targeting proton-pumping ATPases such as V-ATPases in cancer cells [3], F- ATPases in bacteria [4], and P-ATPases in yeas...
Development of eugenol derivatives with 5-LOX inhibitory activity
Filho, José L. Pereira; Pereira, Renato B.; Vieira, Tatiana F.; Sousa, Sérgio F.; Coelho, José Ricardo Alves; Pinto, Nuno F. S.; Coelho, Catarina M. M.Eugenol (4-allyl-2-methoxyphenol), is the major chemical constituent in the essential oil of numerous plant species. Several biological properties have been described for this molecule, including modulation of enzymatic targets relevant for the inflammatory response, such as 5-lipoxygenase (5-LOX). As so, there is interest in expanding the chemical space of this molecule to develop new molecules to be used in i...
Evaluation of the impact of two C5 genetic variants on C5-eculizumab complex st...
Peixoto, Vanda; Prudêncio, Cristina; Vieira, Mónica; Sousa, Sérgio F.Complement C5 is the target of the monoclonal antibody eculizumab, used in complement dysregulating disorders, like the rare disease Paroxysmal Nocturnal Hemoglobinuria (PNH). PNH is an acquired hematopoietic stem cell condition characterized by aberrant destruction of erythrocytes, chronic hemolytic anemia, and thromboembolism propensity. C5 is a protein component of the complement system which is part of the ...
The fungicide cymoxanil impairs respiration in Saccharomyces cerevisiae via cyt...
Mendes, Filipa Diana Silva; Santos-Pereira, Cátia; Vieira, Tatiana F.; Pinto, Mélanie Martins; Castro, Bruno B.; Sousa, Sérgio F.; Sousa, Maria JoãoCymoxanil (CYM) is a widely used synthetic acetamide fungicide, but its biochemical mode of action remains elusive. Since CYM inhibits cell growth, biomass production, and respiration in Saccharomyces cerevisiae, we used this model to characterize the effect of CYM on mitochondria. We found it inhibits oxygen consumption in both whole cells and isolated mitochondria, specifically inhibiting cytochrome c oxidase...
In vitro and in silico evaluation of 5-MeO-DMT, LSD, and mescaline’s interactio...
Brito-da-Costa, Andreia Machado; Carvalho, Mariana; Dinis-Oliveira, Ricardo Jorge; Madureira-Carvalho, Áurea; Sousa, Sérgio F.; Silva, Diana Dias da5-Methoxy-N,N-dimethyltryptamine(5-MeO-DMT), lysergic acid diethylamide (LSD), and mescaline are classic hallucinogens known for their recreational use, which increased in the last dec-ades. Despite some available data on the metabolism of these drugs [1-3], a scientific gap exists regarding their possible interactions with CYP450 enzymes. Nevertheless, this information is of crucial relevance to predict drug-d...
In vitro and in silico evaluation of psilocybin and psilocin’s interaction with...
Brito-da-Costa, Andreia Machado; Carvalho, Mariana; Dinis-Oliveira, Ricardo Jorge; Madureira-Carvalho, Áurea; Sousa, Sérgio F.; Silva, Diana Dias daPsilocybin is a hallucinogen produced by “magic mushrooms”, being rapidly metabolized in the organism into psilocin [1, 2]. A scientific gap exists regarding the possible interactions between psilocybin/psilocin and CYP450 enzymes. Given their biological importance, and since the binding of drugs to CYP450 enzymes can interfere with the metabolism of other substrates leading to drug-drug interactions, this rese...
Heterologous expression and structure prediction of a xylanase identified from ...
Sousa, Joana; Santos-Pereira, Cátia; Dias, Joana Sofia Gomes; Costa, Ângela Maria Araújo; Santos, Andréia O.; Franco-Duarte, RicardoXylanases are key biocatalysts in the degradation of the 1,4glycosidic linkages in the xylan backbone of hemicellulose. These enzymes are potentially applied in a wide range of bioprocessing industries under harsh conditions. Metagenomics has emerged as powerful tools for the bioprospection and discovery of interesting bioactive molecules from extreme ecosystems with unique features, such as high temperatures. ...
LC-ESI-UHR-QqTOF-MS/MS profiling and anti-inflammatory potential of the cultiva...
Zeghbib, Walid; Boudjouan, Fares; Carneiro, João; Oliveira, Ana L. S.; Sousa, Sérgio F.; Pintado, Manuela Estevez; Ourabah, Asma; Vasconcelos, VitorOpuntia plants are abundant but still underexplored edible resources of the Algerian region. This work chemically characterizes extracts of different parts of the fruit of the commercial Opuntia ficus-indica (L.) Mill. and the wild Opuntia stricta (Haw.) Haw. growing in Bejaia, and evaluates their anti-inflammatory potential through different cell and cell-free bioassays. The LC-ESI-UHR-QqTOF-MS/MS analysis ena...
The buzz on insecticides: a review of uses, molecular structures, targets, adve...
Araújo, Maria F.; Castanheira, Elisabete M. S.; Sousa, Sérgio F.Insecticides play a critical role in controlling the spread of insect-borne diseases and preserving crop health. These chemical substances are specifically formulated to kill or manage insect populations. Over the years, various types of insecticides have been developed, including organophosphates, carbamates, pyrethroids, and neonicotinoids, each with unique modes of action, physiological targets, and efficacy...
Cymoxanil inhibits respiration through inhibition of mitochondrial complex IV
Mendes, Filipa; Vieira, Tatiana F.; Pereira, Cátia Sofia Santos; Pinto, Mélanie Martins; Castro, Bruno B.; Sousa, Sérgio F.; Sousa, Maria JoãoCymoxanil is a synthetic acetamide fungicide, used against oomycetes. It was first introduced in 1977 and can be used against downy mildew diseases induced by Plasmopara viticola in grapevine cultures and late blight diseases caused by Phytophthora infestans, in tomatoes and potatoes cultures. This fungicide is used in mixed formulations and its higher solubility enables a relatively widespread occurrence in to...