3 documents found, page 1 of 1
Ionic-liquid-based aqueous biphasic systems with controlled ph: the ionic liqui...
Ventura, Sónia P. M.; Sousa, Sílvia G.; Serafim, Luísa S.; Lima, Álvaro S.; Freire, Mara G.; Coutinho, João A. P.This work addresses the effect of different anions in imidazolium-based ionic liquids (ILs) through the formation of aqueous biphasic systems (ABS) using a phosphate buffer solution (KH 2PO 4/K 2HPO 4 mixture of salts) aiming at controlling the pH value of the coexisting aqueous phases. Twelve ABS were investigated and the corresponding solubility curves were determined by the cloud point titration method at 29...
Ionic liquid based aqueous biphasic systems with controlled pH: the ionic liqui...
Ventura, Sónia P. M.; Sousa, Sílvia G.; Serafim, Luísa S.; Lima, Álvaro S.; Freire, Mara G.; Coutinho, João A. P.This work addresses the evaluation of the ability of ionic liquid cations on the formation of aqueous biphasic systems (ABSʼs), with K₂HPO₄ or a mixture of inorganic salts, K₂HPO₄/KH₂PO₄, aiming at controlling the pH values of the coexisting aqueous phases. Using chloride-based ionic liquids, the effects of the cation core, the length of the alkyl side chain, and the positional isomerism on ABS formation abilit...
Design of ionic liquids for lipase purification
Ventura, Sónia P. M.; Sousa, Sílvia G.; Freire, Mara G.; Serafim, Luísa S.; Lima, Álvaro S.; Coutinho, João A. P.Aqueous two-phase systems (ATPS) are considered as efficient downstream processing techniques in the production and purification of enzymes, since they can be considered harmless to biomolecules due to their high water content and due to the possibility of maintaining a neutral pH value in the medium. A recent type of alternative ATPS is based on hydrophilic ionic liquids (ILs) and salting-out inducing salts. T...