11 documents found, page 1 of 2
Higher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin ...
Souza, Daniel Menezes; Cota, Renata Guerra de Sá; Carneiro, Cláudia Martins; Souza, Juliana Vitoriano de; Giunchetti, Rodolfo CordeiroBackground: The immune response in the skin of dogs infected with Leishmania infantum is poorly understood, and limited studies have described the immunopathological profile with regard to distinct levels of tissue parasitism and the clinical progression of canine visceral leishmaniasis (CVL). Methodology/Principal Findings: A detailed analysis of inflammatory cells (neutrophils, eosinophils, mast cells, lympho...
Clinical, hematological and biochemical alterations in hamster (Mesocricetus au...
Moreira, Nádia das Dores; Souza, Juliana Vitoriano de; Roatt, Bruno Mendes; Vieira, Paula Melo de Abreu; Vital, Wendel CouraBackground: Leishmaniasis remains among the most important parasitic diseases in the developing world and visceral leishmaniasis (VL) is the most fatal. The hamster Mesocricetus auratus is a susceptible model for the characterization of the disease, since infection of hamsters with L. infantum reproduces the clinical and pathological features of human VL. In this context, it provides a unique opportunity to stu...
Kinetics of cell migration to the dermis and hypodermis in dogs vaccinated with...
Souza, Juliana Vitoriano de; Reis, Alexandre Barbosa; Moreira, Nádia das Dores; Giunchetti, Rodolfo Cordeiro; Oliveira, Rodrigo Corrêa deThe search for new immunobiologicals against canine visceral leishmaniasis (CVL) has intensified in the last decade. However, it still remains to be elucidated that mechanisms of the innate immune response in situ after immunization (a.i.). The aim of this study was to investigate the kinetics of cell migration in the skin dogs with distinct antigenic compounds of the LBSap vaccine. Our major findings indicated...
Parasite burden in hamsters infected with two different strains of Leishmania (...
Moreira, Nádia das Dores; Souza, Juliana Vitoriano de; Roatt, Bruno Mendes; Vieira, Paula Melo de Abreu; Ker, Henrique GamaTo develop and test new therapeutics and immune prophylaxis strategies for visceral leishmaniasis (VL), understanding tissue parasitism evolution after experimental infection with Leishmania infantum is important. Experimental infection in a hamster model (Mesocricetus auratus) reproduces several typical aspects of canine and human VL that are closely related to the inoculum’s route. We quantified the parasitis...
Dogs immunized with LBSap vaccine displayed high levels of IL-12and IL-10 cytok...
Souza, Juliana Vitoriano de; Moreira, Nádia das Dores; Souza, Daniel Menezes; Roatt, Bruno Mendes; Soares, Rodrigo Dian de Oliveira AguiarThe complex interplay between cytokines and chemokines regulates innate and adaptive immuneresponses against pathogens; specifically, cytokine and chemokine expression drives activation ofimmune effector cells and their recruitment to tissue infection sites. Herein, we inoculated dogs withLeishmania braziliensis antigens plus saponin (the LBSap vaccine), as well as with the vaccine components,and then used real...
Humoral and cellular immune responses in dogs with inapparent natural Leishmani...
Vital, Wendel Coura; Marques, Marcos José; Giunchetti, Rodolfo Cordeiro; Carvalho, Andréa Teixeira de; Moreira, Nádia das DoresMolecular analysis, serology and immunophenotyping for T lymphocytes and their subsets, B lymphocytes and monocytes were performed on dogs naturally infected with Leishmania infantum. Animals were categorised as asymptomatic dogs I (AD-I), with negative serology and positive molecular results, and asymptomatic dogs II (AD-II), with positive serology and positive molecular results, and these were compared to sym...
Performance of LBSap vaccine after intradermal challenge with L. infantum and s...
Roatt, Bruno Mendes; Soares, Rodrigo Dian de Oliveira Aguiar; Souza, Juliana Vitoriano de; Vital, Wendel Coura; Braga, Samuel LeôncioIn the last decade, the search for new vaccines against canine visceral leishmaniasis has intensified. However, the pattern related to immune protection during long periods after experimental infection in vaccine trials is still not fully understood. Herein, we investigated the immunogenicity and parasitological levels after intradermal challenge with Leishmania infantum plus salivary gland extract in dogs immu...
Cinética de migração celular e expressão da enzima óxido nítrico sintase induzi...
Souza, Juliana Vitoriano deA busca por novos imunobiológicos contra LVC, capazes de ativar uma resposta imune protetora e duradoura no cão, tem se intensificado na última década. No entanto, ainda são poucos os trabalhos que explicam os mecanismos iniciais da resposta inata pós-imunização (p.i) in situ. O presente estudo teve como objetivo central avaliar a cinética de migração celular na pele de cães frente aos distintos componentes ant...
Histological study of cell migration in the dermis of hamsters after immunisati...
Moreira, Nádia das Dores; Giunchetti, Rodolfo Cordeiro; Carneiro, Cláudia Martins; Souza, Juliana Vitoriano de; Roatt, Bruno MendesVaccine candidates, including live and/or killed parasites, Leishmania -purified fractions, defined recombinant antigens and antigen-encoding DNA-plasmids have been proposed to use as vaccine anti- Leishmania . More recently, the hamsters have been used to pre selection of antigens candidate to apply in further experiments using canine model. In this report we evaluated the kinetics of cell migration in dermal ...
Cell recruitment and cytokines in skin mice sensitized with the vaccine adjuvan...
Souza, Juliana Vitoriano de; Moreira, Nádia das Dores; Carvalho, Andréa Teixeira de; Carneiro, Cláudia Martins; Siqueira, Fernando Augusto MathiasVaccine adjuvants are substances associated with antigens that are fundamental to the formation of an intense, durable, and fast immune response. In this context, the use of vaccine adjuvants to generate an effective cellular immune response is crucial for the design and development of vaccines against visceral leishmaniasis. The objective of this study was to evaluate innate inflammatory response induced by th...