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Alternative Heterologous Expression of l‑Arabinose Isomerase from Enterococcus ...

Souza, Ticiane C. de; Oliveira, Ravena Casemiro; Bezerra, Saulo Gonçalves Santiago; Manzo, Ricardo M; Mammarella, Enrique J; Hissa, Denise Cavalcante

This study reports an alternative strategy for the expression of a recombinant l-AI from Enterococcus faecium DBFIQ E36 by auto-induction using glucose and glycerol as carbon sources and residual whey lactose as inducer agent. Commercial lactose and isopropyl β-d-1-thiogalactopyranoside (IPTG) were also evaluated as inducers for comparison of enzyme expression levels. The enzymatic extracts were purifed by afni...

Date: 2021   |   Origin: Oasisbr
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