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Machine learning assisted classification of staphylococcal biofilm maturity
van Dun, S.C.J.; Knol, R.; Silva-Herdade, Ana S.; Veiga, Ana Salomé; Castanho, Miguel A. R. B.; Nibbering, P.H.; Pijls, B.G.C.W.; van der Does, A.M.An increasing incidence of device-related, biofilm-associated infections has been observed in clinical practice worldwide. In vitro biofilm models are essential to study these burdensome infections and to design and test potential new treatment approaches. However, there is considerable variation in in vitro biofilm models, and a generally accepted systematic description of biofilm maturity - apart from incubat...
Improved Triamcinolone acetonide-eluting contact lenses based on cyclodextrins ...
Marto-Costa, Carolina; Toffoletto, Nadia; Salema-Oom, Madalena; Antunes, Alexandra M. M.; Pinto, Carlos A.; Saraiva, Jorge A.; Silva-Herdade, Ana S.Contact lenses (CLs) constitute an advantageous platform for the topical release of corticosteroids due to their prolonged contact with the eye. However, the lipophilic nature of corticosteroids hampers CLs' ability to release therapeutic amounts. Two approaches to improve loading and release of triamcinolone acetonide (TA) from poly(2-hydroxyethyl methacrylate)-based hydrogels were investigated: adding 2-hydro...
Improved triamcinolone acetonide-eluting contact lenses based on cyclodextrins ...
Marto-Costa, Carolina; Toffoletto, Nadia; Salema-Oom, Madalena; Antunes, Alexandra M.M.; Pinto, Carlos A.; Saraiva, Jorge A.; Silva-Herdade, Ana S.Contact lenses (CLs) constitute an advantageous platform for the topical release of corticosteroids due to their prolonged contact with the eye. However, the lipophilic nature of corticosteroids hampers CLs' ability to release therapeutic amounts. Two approaches to improve loading and release of triamcinolone acetonide (TA) from poly(2-hydroxyethyl methacrylate)-based hydrogels were investigated: adding 2-hydro...
Towards non-invasive intravital microscopy: advantages of using the ear lobe in...
Mota-Silva, Iara; Castanho, Miguel A. R. B.; Silva-Herdade, Ana S.Inflammation is essential in the protection of the organism and wound repair, but in cases of chronic inflammation can also cause microvasculature deterioration. Thus, inflammation monitorization studies are important to test potential therapeutics. The intravital microscopy (IVM) technique monitors leukocyte trafficking in vivo, being a commonly used procedure to report systemic conditions. Although the cremas...
A designed cyclic analogue of gomesin has potent activity against Staphylococcu...
Dias, Susana; Pinto, Sandra; Silva-Herdade, Ana S.; Cheneval, Olivier; Craik, David J; Coutinho, Ana; Castanho, Miguel A. R. B.; Henriques, Sónia T.Background: Infections caused by bacterial biofilms are very difficult to treat. The use of currently approved antibiotics even at high dosages often fails, making the treatment of these infections very challenging. Novel antimicrobial agents that use distinct mechanisms of action are urgently needed. Objectives: To explore the use of [G1K,K8R]cGm, a designed cyclic analogue of the antimicrobial peptide gomesin...
Functional food components, intestinal permeability and inflammatory markers in...
Lacerda, Joana Franco; Lagos, Ana Catarina; Carolino, Elisabete; Silva-Herdade, Ana S.; Silva, Manuel; Guerreiro, Catarina SousaInflammatory bowel diseases (IBD) are characterized by a chronic inflammatory process that affects the intestinal barrier structure. Recent evidence suggests that some food components can influence the integrity of the intestinal barrier and thus its permeability. We aimed at assessing the effect of food components on the intestinal permeability (IP) and on inflammatory markers in individuals with IBD by a sing...
Retinal vascular reactivity in type 1 diabetes patients without retinopathy usi...
Sousa, David Cordeiro; Leal, Ines; Moreira, Susana; Vale, Sónia do; Silva-Herdade, Ana S.; Aguiar, Patrício; Dionísio, Patrícia; Pinto, LuisPurpose: We hypothesize that patients with type 1 diabetes (T1D) may have abnormal retinal vascular responses before diabetic retinopathy (DR) is clinically evident. Optical coherence tomography angiography (OCTA) was used to dynamically assess the retinal microvasculature of diabetic patients with no clinically visible retinopathy. Methods: Controlled nonrandomized interventional study. The studied population ...
Effects of diabetes on microcirculation and leukostasis in retinal and non-ocul...
Silva-Herdade, Ana S.; Mota-Silva, Iara; Calado, Ângelo; Saldanha, Carlota; Nguyen, Ngan-Ha; Hou, Isabella; Castanho, Miguel A. R. B.; Roy, SayonChanges in retinal microcirculation are associated with the development of diabetic retinopathy (DR). However, it is unclear whether such changes also develop in capillary beds of other non-retinal tissues. Here, we investigated microcirculatory changes involving velocity of rolling neutrophils, adherence of neutrophils, and leukostasis during development of retinal vascular lesions in diabetes in other non-ret...
Optical coherence tomography angiography study of the retinal vascular plexuses...
Sousa, David Cordeiro; Leal, Ines; Moreira, Susana; Vale, Sónia do; Silva-Herdade, Ana S.; Dionísio, Patrícia; Castanho, Miguel A. R. B.; Pinto, LuisAim Previous data suggest the existence of retinal vascular changes and impaired autoregulation in the very early stages of diabetic retinopathy (DR). We compared the retinal plexuses between patients with type 1 diabetes (T1D) without DR and a demographically similar healthy cohort, using optical coherence tomography angiography (OCT-A). Methods Patients with T1D and no signs of DR were prospectively recruited...
A protocol to evaluate retinal vascular response using optical coherence tomogr...
Sousa, David Cordeiro; Leal, Ines; Moreira, Susana; Vale, Sónia do; Silva-Herdade, Ana S.; Aguiar, Patrício; Dionísio, Patrícia; Pinto, LuisIntroduction: Optical coherence tomography angiography (OCT-A) is a novel diagnostic tool with increasing applications in ophthalmology clinics that provides non-invasive high-resolution imaging of the retinal microvasculature. Our aim is to report in detail an experimental protocol for analyzing both vasodilatory and vasoconstriction retinal vascular responses with the available OCT-A technology. Methods: A co...