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Molecular cloning and expression analysis of three omega-6 desaturase genes fro...

Teixeira, Monica; Coelho, N.; Olsson, M. E.; Brodelius, P. E.; Carvalho, Isabel Saraiva de; Brodelius, Maria

Two full-length cDNA clones of PoleFAD2 and one full-length cDNA clone of PoleFAD6, encoding omega-6 fatty acid desaturases, the key enzymes for the conversion of oleic into linoleic acid, were isolated from purslane (Portulaca oleracea L.) leaves and seeds. The deduced amino acid sequence of both isoforms of PoleFAD2 showed higher similarities to other microsomal omega-6 desaturases then to PoleFAD6 or other p...


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