3 documents found, page 1 of 1
Direct Assay to Evaluate Phosphoenolpyruvate Carboxykinase Activity
Amaral,Bruno S. do; Correa,Katia Celina S.; Corrêa,Arlene G.; Souza,Dulce H. F. de; Cass,Quezia B.Phosphoenolpyruvate carboxykinase (PEPCK) is a ubiquitous enzyme found in all known groups of organisms, acting in the reversible conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) in the presence of divalent metal ion, and dependent of adenosine 5’-triphosphate (ATP) or guanosine-5’-triphosphate (GTP). PEPCK is an important enzyme in the metabolism of some organisms, such as Trypanosoma cruzi, being...
Leishmanicidal galloylquinic acids are noncompetitive inhibitors of arginase
Sousa,Lorena R. F. de; Ramalho,Suelem D.; Fernandes,João B.; Silva,Maria Fátima das G. F. da; Iemma,Mônica R. da C.; Corrêa,Caroindes J.Leishmanicidal galloylquinic acids were isolated from the ethyl acetate extract of Byrsonima coccolobifolia. These phenols and gallic acid showed to be a new class of potent noncompetitive inhibitors of arginase ARG (Ki ranging from 0.10 to 0.68 µmol L-1) from Leishmania amazonensis. Quinic acid did not exhibit significant inhibition of ARG, indicating that galloyl moiety has important features that allows the ...
Enzymatic inhibition studies of selected flavonoids and chemosystematic signifi...
Moraes,Valéria R. de S.; Tomazela,Daniela M.; Ferracin,Ricardo J.; Garcia,Cleverson F.; Sannomiya,Míriam; Soriano,M. del Pilar C.Our taxonomic interest in the Neoraputia stimulated an investigation of N. paraensis searching for alkaloids. Fractions were monitored by ¹H NMR and ESI-MS/MS and only those which showed features of anthranilate alkaloids and flavonoids absent in the previous investigations were examined. Stems afforded the alkaloids flindersine, skimmianine, 8-methoxyflindersine and dictamnine; leaves yielded 3',4',7,8-tetrame...