4 documents found, page 1 of 1
Cryopreservation of Mycobacterium bovis isolates
Ikuta, Cássia Yumi; Ambrosio, Simone Rodrigues; Souza Filho, Antonio Francisco de; Grisi-Filho, José Henrique de Hildebrand; Heinemann, Marcos BryanResearch, development of new biotechnological methods, diagnostic tests, confirmation of results, and reinvestigations are possible because of the availability of well-preserved living organisms maintained without any changes. Cryopreservation is a simpler, more reliable and long-term stable method for culture maintenance. Storage temperature and composition of the suspending vehicle are factors that affect the...
Comparison of DNA extraction protocols to detect Mycobacterium bovis in bovine ...
Ikuta, Cássia Yumi; Oliveira, Daniella Carvalho Ribeiro; Souza, Gisele Oliveira de; Souza Filho, Antonio Francisco deThe current scenario of international beef trading has increased the pressure for better and faster diagnosis of bovine tuberculosis. Although traditional culture remains the gold standard method to confirm Mycobacterium bovis infection, it is exceedingly time consuming, and demands viable mycobacteria. Molecular methods overcome the flaws of the bacteriological methods with faster detection and identification....
Genetic profiles of Mycobacterium bovis from a cattle herd in southernmost Brazil
Souza Filho, Antonio Francisco de; Osório, Ana Luiza Alves Rosa; Jorge, Klaudia dos Santos Gonçalves; Araújo, Flábio Ribeiro; Vidal, Carlos Eugênio SotoMycobacterium bovis is the agent of bovine tuberculosis, a disease endemic to all Brazilian states. Molecular typing techniques help to stratify and refine data, providing information that facilitates epidemiological research. In this study, MIRU-VNTR, targeting 24 loci, was employed to identify and characterize the genetic groups of M. bovis isolates obtained from an outbreak of bovine tuberculosis. Eighteen a...
Genotipagem pela técnica MIRU-VNTR de Mycobacterium bovis isolados de tecido bo...
Souza Filho, Antonio Francisco deMycobacterium bovis, um membro do complexo Mycobacterium tuberculosis, é o agente mais comum da tuberculose bovina. Além dos bovinos pode acometer outros animais domésticos, animais silvestres e o homem. Enfermidade endêmica no Brasil, cujo controle se tornou alvo de exigências sanitárias internacionais. Avanços nas técnicas de tipagem molecular têm contribuído para o estudo epidemiológico da tuberculose bovina...