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Aquaporins Transcripts with Potential Prognostic Value in Pancreatic Cancer

Lopes, PA; Fonseca, E; da Silva, IV; Vigia, E; Paulino, J; Soveral, G

Pancreatic cancer is anticipated to be the second leading cause of cancer-related death by 2030. Aquaporins (AQPs), a family of water channel proteins, have been linked to carcinogenesis. The aim of this study was to determine AQP gene expression in pancreatic cancer tissues and to validate aquaporins as possible diagnosis and/or prognosis genes. The relative gene expression levels of AQP1, AQP3, AQP5, and AQP9...


Sweet cherry (Prunus avium L.) PAPIP1;4 is a functional aquaporin upregulated b...

Breia, R; Mósca, AF; Conde, A; Correia, S; Conde, C; Noronha, H; Soveral, G; Gonçalves, B; Gerós, H

The involvement of aquaporins in rain-induced sweet cherry (Prunus avium L.) fruit cracking is an important research topic with potential agricultural applications. In the present study, we performed the functional characterization of PaPIP1;4, the most expressed aquaporin in sweet cherry fruit. Field experiments focused on the pre-harvest exogenous application to sweet cherry trees, cultivar Skeena, with a sol...


Differential Expression of Aquaporin-3 and Aquaporin-5 in Pancreatic Ductal Ade...

Direito, I; Paulino, J; Vigia, E; Brito, MA; Soveral, G

Background and objectives: Aquaporin-5 (AQP5) and -3 (AQP3) are protein channels that showed to be up-regulated in a variety of tumors. Our goal was to investigate the expression pattern of AQP5 and AQP3 in pancreatic ductal adenocarcinomas (PDA) and correlate with cell proliferation, tumor stage and progression, and clinical significance. Methods: 35 PDA samples in different stages of differentiation and locat...



Heterologous expression, purification and crystallization of the human mitochon...

Violante, S; Gaspar, MM; Cruz, MEM; Gomes, C; Carvalho, AL; Romão, MJ; Wanders, RJA; Soveral, G; Leandro, P; Tavares de Almeida, I; Ventura, FV


Strategy for the Characterization of a Human Membrane Membrane Protein

Ventura, FV; Violante, S; Gaspar, MM; Carvalho, AL; Cruz, MEM; Romão, MJ; Soveral, G; Wanders, RJA; Leandro, P; Tavares de Almeida, I


Optimization of bacterial overexpression of human carnitine acylcarnitine trans...

Ventura, F; Violante, S; Jorge, AM; Gaspar, MM; Cruz, MEM; Soveral, G; Leandro, P; Tavares de Almeida, I


Water channels are important for osmotic adjustments of yeast cells at low temp...

Soveral, G; Veiga, A; Loureiro-Dias, MC; An, TH; Van Dijck, P; Moura, TF

The importance of aquaporin expression in water permeability in Saccharomyces cerevisiae was assessed by measuring the osmotic water permeability coefficient (P-f) and the activation energies (E-a) from both hypo- and hypertonic experiments performed with whole protoplasts from four strains differing in aquaporin level of expression: parental, double-deleted and overexpressing AQY1 or AQY2. Double-deleted (lowe...



Heterologous expression of the human carnitine acylcarnitine translocase (CACT)

Ventura, FV; Violante, S; Jorge, AM; IJlst, L; Gaspar, MM; van Roermund, CWT; Cruz, MEM; Soveral, G; Wanders, RJA; Leandro, P; Tavares de Almeida, I


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