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Fungicide application can improve production of tomato coinfected with Begomovi...
Guimarães,Leysimar Ribeiro Pitzr; Nozaki,Denise Nakada; Moura,Mônika Fecury; Spadotti,David Marques de Almeida; Mituti,Tatiana; Krause-Sakate,RenateAbstract: The objective of this work was to evaluate the effect of fungicide application on the concentration of Tomato severe rugose virus (ToSRV, Begomovirus) in the 'Mariana' hybrid tomato coinfected with ToSRV and Tomato chlorosis virus (ToCV, Crinivirus) and the progression of viral concentration by qPCR, as well as to quantify fruit yield and quality. Experiment I consisted in the application of fungicide...
Efficient detection of Frankliniella schultzei (Thysanoptera, Thripidae) by cyt...
Leão,Evelynne Urzêdo; Spadotti,David Marques de Almeida; Rocha,Kelly Cristina G.; Lima,Élison Fabricio B.; Tavella,Luciana; Turina,MassimoABSTRACT Identification of Thysanoptera is based mainly on external morphology examination that can be time-consuming and difficult for non taxonomic experts. In this work, we propose a rapid and efficient molecular method to identify Frankliniella schultzei, an important and widespread pest thrips vector of tospoviruses in South America countries. Species-specific primers designed in the mitochondrial cytochro...
The wild type of Momordica charantia is not infected by potyviruses that cause ...
Spadotti,David Marques de Almeida; Buriolla,José Edivaldo; Rezende,Jorge Alberto Marques; Souza,Vinicius CastroIn the present work, the susceptibility of wild and domesticated plants of Momordica charantia to viruses from the genus Potyvirus that cause diseases in papaya (Carica papaya) and cucurbit crops was evaluated. The plants were subjected to experimental and natural infection with Papaya ringspot virus types P and W (PRSV-P and PRSV-W) and Zucchini yellow mosaic virus (ZYMV). None of the potyviruses infected the ...
Levantamento revela a predominância do Lettuce mottle virus em três regiões pro...
De Marchi,Bruno Rossitto; Spadotti,David Marques de Almeida; Oliveira,Milena Leite de; Sanches,Márcio Martinello; Krause-Sakate,RenateEntre os problemas fitossanitários da cultura da alface estão as doenças causadas por vírus. Três vírus causam sintomas de mosaico praticamente indistinguíveis: o Lettuce mosaic virus (LMV, Potyvirus), o Lettuce mottle virus (LeMoV, Sequivirus) e o Bidens mosaic virus (BiMV, Potyvirus). Através de RT-PCR utilizando-se oligonucleotídeos específicos para cada um destes vírus, amostras de alface e plantas invasora...
Bidens mosaic virus: detecção via RT-PCR e identificação de Galinsoga parviflor...
Sanches,Márcio Martinello; Spadotti,David Marques de Almeida; De Marchi,Bruno Rossito; Pavan,Marcelo Agenor; Krause-Sakate,RenateO Bidens mosaic virus (BiMV) é uma espécie tentativa do gênero Potyvirus, que infecta alface (Lactuca sativa). Na ausência de métodos eficientes para diagnose deste vírus, o objetivo do trabalho foi a síntese de oligonucleotídeos específicos e sua otimização em testes de RT-PCR em uma só etapa, partindo-se de extrações de RNA total. Os oligonucleotídeos 8851sens (5'AGG CAG TTC GCA CGG CAT AC 3´) e 9211ant (5´ C...