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Preserving accuracy in GenBank

Bidartondo, M. I.; Bruns, T. D.; Blackwell, M.; Edwards, I.; Taylor, A. F. S.; Horton, T.; Zhang, N.; Koljalg, U.; May, G.; Kuyper, T. W.; Gilbert, G.

GenBank, the public repository for nucleotide and protein sequences, is a critical resource for molecular biology, evolutionary biology, and ecology. While some attention has been drawn to sequence errors (1), common annotation errors also reduce the value of this database. In fact, for organisms such as fungi, which are notoriously difficult to identify, up to 20% of DNA sequence records may have erroneous lin...


Reconstructing the early evolution of Fungi using a six-gene phylogeny

James, T. Y.; Kauff, F.; Schoch, C. L.; Matheny, P. B.; Hofstetter, V.; Cox, C. J.; Celio, G.; Gueidan, C.; Fraker, E.; Miadlikowska, J.; Lumbsch, H. T.

The ancestors of fungi are believed to be simple aquatic forms with flagellated spores, similar to members of the extant phylum Chytridiomycota (chytrids). Current classifications assume that chytrids form an early-diverging clade within the kingdom Fungi and imply a single loss of the spore flagellum, leading to the diversification of terrestrial fungi. Here we develop phylogenetic hypotheses for Fungi using d...


Assembling the fungal tree of life: progress, classification, and evolution of ...

Lutzoni, F.; Kauff, F.; Cox, C. J.; McLaughlin, D.; Celio, G.; Dentinger, C.; Padamsee, M.; Hibbett, D. S.; James, T. Y.; Baloch, E.; Grube, M.

Based on an overview of progress in molecular systematics of the true fungi(Fungi/Eumycota) since 1990, little overlap was found among single-locus data matrices, which explains why no large-scale multilocus phylogenetic analysis had been undertaken to reveal deep relationships among fungi. As part of the project ‘‘Assembling the Fungal Tree of Life’’ (AFTOL), results of four Bayesian analyses are reported with...


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