4 documents found, page 1 of 1
Xenopus Pkdcc1 and Pkdcc2 Are Two New Tyrosine Kinases Involved in the Regulati...
Vitorino, Marta; Silva, Ana Cristina; Inacio, Jose Manuel; Ramalho, Jose Silva; Gur, Michal; Fainsod, Abraham; Steinbeisser, Herbert; Belo, José A.Protein Kinase Domain Containing, Cytoplasmic (PKDCC) is a protein kinase which has been implicated in longitudinal bone growth through regulation of chondrocytes formation. Nevertheless, the mechanism by which this occurs remains unknown. Here, we identified two new members of the PKDCC family, Pkdcc1 and Pkdcc2 from Xenopus laevis. Interestingly, our knockdown experiments revealed that these two proteins are ...
Study of Xenopus orthologs of novel genes expressed in the mouse AVE
Becker, Jorg D.; Steinbeisser, Herbert; Belo, A.; Silva, Ana C.; Vitorino, Marta; Filipa, Mário; Marques, Sara
Developmental expression of Shisa-2 in Xenopus laevis
Silva, Ana-Cristina; Filipe, Mario; Vitorino, Marta; Steinbeisser, Herbert; Belo, José A.Shisa is an antagonist of Wnt and FGF signaling, that functions cell autonomously in the endoplasmic reticulum (ER) to inhibit the post-translational maturation of Wnt and FGF receptors. In this paper we report the isolation of a second Xenopus shisa gene (Xshisa-2). Xenopus Shisa-2shows 30.7% identity to Xshisa. RT-PCR analysis indicated that Xshisa-2 mRNA is present throughout early development and shows an i...
Endogenous Cerberus activity is required for anterior head specification in Xen...
Silva, Ana Cristina; Filipe, Mário; Kuerner, Klaus-Michael; Steinbeisser, Herbert; Belo, José A.We analyzed the endogenous requirement for Cerberus in Xenopus head development. 'Knockdown' of Cerberus function by antisense morpholino oligonucleotides did not impair head formation in the embryo. In contrast, targeted increase of BMP, Nodal and Wnt signaling in the anterior dorsal-endoderm (ADE) resulted in synergistic loss of anterior head structures, without affecting more posterior axial ones. Remarkably...