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In vitro and in silico characterization of a novel dextranase from Pochonia chl...

Sufiate, Bruna; Araújo, Jackson Victor de; Queiroz, José Humberto de; Gouveia, Angélica S; Cardoso, Evandro; Moreira, Samara Silveira

The objective of this study was to purify, characterize, and phylogenetically and structurally analyze the dextranase produced by the fungus Pochonia chlamydosporia. Dextranase produced by the fungus P. chlamydosporia was purified to homogeneity in two steps, with a yield of 152%, purification factor of 6.84 and specific activity of 358.63 U/mg. Its molecular weight was estimated by SDS-PAGE at 64 kDa. The enzy...

Date: 2019   |   Origin: Oasisbr
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