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Virulence factor of Candida tropicalis isolated from hospitalized patients

Negri, M.; Henriques, Mariana; Svidzinski, T.; Azeredo, Joana; Oliveira, Rosário

Candida tropicalis has been reported to be one of the Candida species which is most likely to cause bloodstream and urinary tract infections in hospitals. Several virulence factors seem to be responsible for C. tropicalis infections, which present high potential for dissemination and mortality. The aim of this study was to investigate the correlation between different virulence factors (enzymes secretion, adhes...


Insights into Candida tropicalis virulence factors

Negri, M.; Henriques, Mariana; Silva, Sónia Carina; Svidzinski, T.; Azeredo, Joana; Oliveira, Rosário

Candida tropicalis is a common nosocomial species related to candidemia and candiduria. Several virulence factors seem to be responsible for C. tropicalis infections, which lead to high mortality. Adhesion to surfaces (medical devices and host cells) and biofilm formation are considered important factors that contribute to the development of candidosis. Therefore, adhesion to urinary catheters and biofilm forma...


Examination of potential virulence factors of Candida tropicalis clinical isola...

Negri, M.; Martins, Margarida Isabel Barros Coelho; Henriques, Mariana; Svidzinski, T.; Azeredo, Joana; Oliveira, Rosário

Candida tropicalis has been reported to be one of the Candida species which is most likely to cause bloodstream and urinary tract infections in hospitalized patients. Accordingly, the aim of this study was to characterize the virulence of C. tropicalis by assessing antifungal susceptibility and comparing the expression of several virulence factors. This study was conducted with seven isolates of C. tropicalis f...


Candida tropicalis biofilms : formation and virulence factors

Oliveira, Rosário; Svidzinski, T.; Henriques, Mariana; Silva, Sónia Carina; Botelho, C. M.; Negri, M.

Significance and objectives: A substantial proportion of Candida tropicalis infections is associated with biofilm formation, especially on catheters. Thus, the aim of this study was to investigate C. tropicalis biofilm formation on silicone and its effect on epithelial cells and enzyme production (hemolysins and proteinases). Methods and results: This study was performed with C. tropicalis (clinical isolate and...


Yeast from urinary nosocomial infection : biofilm and susceptibility to antifun...

Freitas, A. R.; Baeza, L C.; Dota, K. F. D.; Negri, M.; Svidzinski, T.

Urinary infections caused by yeasts of Candida genus, in hospital environment, are frequent. The object of this work was to evaluate the susceptibility profile of yeasts isolated in patients with urinary infection to antifungal agents, comparing with broth methods microdilution and disk diffusion, and it was evaluated the capacity of these yeasts to form biofilm as well. There were used 98 samples isolated from...


Effect of Candida tropicalis biofilm on urinary epithelial cells

Negri, M.; Henriques, Mariana; Lopes, Luís Miguel Reis Henriques; Svidzinski, T.; Azeredo, Joana; Oliveira, Rosário


Effect of Candida tropicalis in planktonic and biofilm form on urinary epitheli...

Negri, M.; Lopes, Luís Miguel Reis Henriques; Henriques, Mariana; Svidzinski, T.; Azeredo, Joana; Oliveira, Rosário


Correlation between Etest®, disk diffusion, and microdilution methods for antif...

Negri, M.; Henriques, Mariana; Svidzinski, T.; Paula, Claudete Rodrigues; Oliveira, Rosário

The correlation between the microdilution (MD), Etest® (ET), and disk diffusion (DD) methods was determined for amphotericin B, itraconazole and fluconazole. The minimal inhibitory concentration (MIC) of those antifungal agents was established for a total of 70 Candida spp. isolates from colonization and infection. The species distribution was: Candida albicans (n=27), C. tropicalis (n=17), C. glabrata (n=16), ...


Candida tropicalis biofilm on latex and silicone catheters

Negri, M.; Henriques, Mariana; Svidzinski, T.; Azeredo, Joana; Oliveira, Rosário


Correlation between E-test, disk difusion, and microdilution methods for antifu...

Negri, M.; Ribeiro, A.; Svidzinski, T.; Henriques, Mariana; Oliveira, Rosário


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