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Remote triggering of TGF-β/Smad2/3 signaling in human adipose stem cells laden ...
Matos, A. M.; Gonçalves, A. I.; Rodrigues, M. T.; Miranda, M. S.; Haj, A. E.; Reis, R. L.; Gomes, Manuela E.Injuries affecting load bearing tendon tissues are a significant clinical burden and efficient treatments are still unmet. Tackling tendon regeneration, tissue engineering strategies aim to develop functional substitutes that recreate native tendon milieu. Tendon mimetic scaffolds capable of remote magnetic responsiveness and functionalized magnetic nanoparticles (MNPs) targeting cellular mechanosensitive recep...
Triggering the activation of Activin A type II receptor in human adipose stem c...
Gonçalves, A. I.; Rotherham, M.; Markides, H.; Rodrigues, M. T.; Reis, R. L.; Gomes, M. E.; Haj, A. J.Stem cell therapies hold potential to stimulate tendon regeneration and homeostasis, which is maintained in response to the native mechanical environment. Activins are members of the mechano-responsive TGF-β superfamily that participates in the regulation of several downstream biological processes. Mechanosensitive membrane receptors such as activin can be activated in different types of stem cells via magneti...
Bi-directional modulation of cellular interactions in an in vitro co-culture mo...
Calejo, I.; Costa-Almeida, R.; Gonçalves, A. I.; Berdecka, D.; Reis, R. L.; Gomes, Manuela E.Objectives: This work aimed at studying in vitro interactions between human tendonâ derived cells (hTDCs) and preâ osteoblasts (preâ OBs) that may trigger a cascade of events involved in enthesis regeneration. Materials and methods The effect of 5 osteogenic medium (OM) conditions over the modulation of hTDCs and preâ OBs towards the tenogenic and osteogenic phenotypes, respectively, was studied. Three differen...
Injectable hyaluronic acid hydrogels enriched with platelet lysate as a cryosta...
Neves, Lisete S.; Babo, Pedro Miguel Sousa; Gonçalves, A. I.; Almeida, Raquel Costa; Caridade, Sofia Glória Ferreira; Mano, J. F.Cell-based regenerative medicine strategies hold a great potential to revolutionize the treatment of a large number of injuries with limited regenerative potential. However, the effectiveness of the simple injection of a cell suspension in a target site/tissue of action is often limited by the dispersion of cells toward other tissues, hindering their therapeutic action. Nevertheless, the development of a custom...
Tissue-engineered magnetic cell sheet patches for advanced strategies in tendon...
Gonçalves, A. I.; Rodrigues, M. T.; Gomes, Manuela E.Tendons are powerful 3D biomechanically structures combining a few cells in an intrincated and highly hierarchical niche environment. When tendon homeostasis is compromised, restoration of functionality upon injury is limited and requires alternatives to current augmentation or replacement strategies. Cell sheet technologies are a powerful tool for the fabrication of living extracellular-rich patches towards re...
Exploring the potential of starch/polycaprolactone aligned magnetic responsive ...
Gonçalves, A. I.; Rodrigues, M. T.; Carvalho, P. P.; Bañobre-López, M.; Paz, E.; Freitas, P.; Gomes, Manuela E.The application of magnetic nanoparticles (MNPs) in tissue engineering (TE) approaches opens several new research possibilities in this field, enabling a new generation of multifunctional constructs for tissue regeneration. This study describes the development of sophisticated magnetic polymer scaffolds with aligned structural features aimed at applications in tendon tissue engineering (TTE). Tissue engineering...
Erratum to chapter 10, ‘Tendon stem cell niche’
Almeida, Raquel Costa; Gonçalves, A. I.; Gershovich, Pavel; Rodrigues, Márcia T.; Reis, R. L.; Gomes, Manuela E.In Chapter 10, the Table of Contents, and the Contributors List, the lead author’s last name was misspelled ‘Costa-Alameida’; it should have been spelled ‘CostaAlmeida’.
In vitro and in vivo assessment of magnetically actuated biomaterials for tendo...
Santos, L. J.; Silva, M.; Gonçalves, A. I.; Pesqueira, T.; Rodrigues, M. T.; Reis, R. L.; Gomes, M. E.Tendon regeneration can be undermined by the formation of fibrous adhesions (scar tissue) between the healing tendon and the surrounding tissues. Scarring is associated with the recruitment of inflammatory cells such as macrophages and mast cells[1]. To tackle this issue we advocate the implementation of postoperative rehabilitation programmes to control inflammation levels and scarring, ensuring this way bette...
Tendon regeneration through a scaffold-free approach: development of tenogenic ...
Gonçalves, A. I.; Rodrigues, M. T.; Reis, R. L.; Gomes, Manuela E.Tendon's regeneration is limited, demanding for cell-based strategies to fully restore their functionality upon injury. The concept of magnetic force-based TE(1), generally using magnetic nanoparticles may enable, for example, stem cell stimulation and/or remote control over TE constructs. Thus, we originally propose the development of magnetic cell sheets (magCSs) with tenogenic capability, aimed at promoting ...
The effect of magnetic stimulation on the osteogenic and chondrogenic different...
Lima, João; Gonçalves, A. I.; Rodrigues, Márcia T.; Reis, R. L.; Gomes, Manuela E.The use of magnetic nanoparticles (MNPs) towards the musculoskeletal tissues has been the focus of many studies, regarding MNPs ability to promote and direct cellular stimulation and orient tissue responses. This is thought to be mainly achieved by mechano-responsive pathways, which can induce changes in cell behavior, including the processes of proliferation and differentiation, in response to external mechani...