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Compounds altering fat storage in Daphnia magna

Jordão, Rita; Garreta, Elba; Campos, Bruno; Lemos, Marco; Soares, Amadeu M.V.M.; Tauler, Romà; Barata, Carlos

The analysis of lipid disruptive effects in invertebrates is limited by our poor knowledge of the lipid metabolic pathways. A recent study showed that tributyltin activated the ecdysteroid, juvenile hormone and retinoic X receptor signaling pathways, and disrupted the dynamics of neutral lipids in the crustacean Daphnia magna impairing the transfer of triacylglycerols to eggs and hence promoting their accumulat...

Date: 2016   |   Origin: IC-online

Induction of multixenobiotic defense mechanisms in resistant Daphnia magna clon...

Jordão, Rita; Campos, Bruno; Lemos, Marco; Soares, Amadeu M.V.M.; Tauler, Romà; Barata, Carlos

Multixenobiotic resistance mechanisms (MXR) were recently identified in Daphnia magna. Previous results characterized gene transcripts of genes encoding and efflux activities of four putative ABCB1 and ABCC transporters that were chemically induced but showed low specificity against model transporter substrates and inhibitors, thus preventing us from distinguishing between activities of different efflux transpo...

Date: 2016   |   Origin: IC-online

Obesogens beyond Vertebrates: Lipid Perturbation by Tributyltin in the Crustace...

Jordão, Rita; Casas, Josefina; Fabrias, Gemma; Campos, Bruno; Piña, Benjamín; Lemos, Marco; Soares, Amadeu M V M; Tauler, Romà; Barata, Carlos

The analysis of obesogenic effects in invertebrates is limited by our poor knowledge of the regulatory pathways of lipid metabolism. Recent data from the crustacean Daphnia magna points to three signaling hormonal pathways related to the molting and reproductive cycles [retinoic X receptor (RXR), juvenile hormone (JH), and ecdysone] as putative targets for exogenous obesogens. Objective: The present study addre...

Date: 2015   |   Origin: IC-online

Transcriptomic, biochemical and individual markers in transplanted Daphnia magn...

Rivetti, Claudia; Campos, Bruno; Faria, Melissa; Català, Nuria De Castro; Malik, Amrita; Muñoz, Isabel; Tauler, Romà; Soares, Amadeu M. V. M.

Daphnia magna individuals were transplanted across 12 sites from three Spanish river basins (Llobregat, Ebro, Jucar) showing different sources of pollution. Gene transcription, feeding and biochemical responses in the field were assessed and comparedwith those obtained in re-constitutedwater treatments spikedwith organic eluates obtained fromwater samples collected at the same locations and sampling periods. Up...


Obesogens beyond vertebrates: lipid perturbation by tributyltin in the crustace...

Jordão, Rita; Casas, Josefina; Fabrias, Gemma; Campos, Bruno; Pina, Benjamín; Lemos, Marco F. L.; Soares, Amadeu M. V. M.; Tauler, Romà; Barata, Carlos

Background: The analysis of obesogenic effects in invertebrates is limited by our poor knowledge of the regulatory pathways of lipid metabolism. Recent data from the crustacean Daphnia magna points to three signaling hormonal pathways related to the molting and reproductive cycles [retinoic X receptor (RXR), juvenile hormone (JH), and ecdysone] as putative targets for exogenous obesogens. Objective: The present...


Identifying major pesticides affecting bivalve species exposed to agricultural ...

Damasio, Joana; Navarro-Ortega, Alícia; Tauler, Romà; Lacorte, Silvia; Barcelo, Damià; Soares, Amadeu M. V. M.; López, Miguel Angel; Riva, Mari Carmen

The aim of this investigation was to identify major pesticides that may cause detrimental effects in bivalve species affected by agricultural pollution. Investigations were carried out using freshwater clams (Corbicula fluminea) transplanted in the main drainage channels that collect the effluents coming from agriculture fields in the Ebro Delta (NE Spain) during the main growing season of rice (from May to Aug...


Combined use of Daphnia magna in situ bioassays, biomarkers and biological indi...

Damásio, Joana; Tauler, Romà; Teixidó, Elisabeth; Rieradevall, Maria; Prat, Narcis; Riva, Maria Carmen; Soares, Amadeu M. V. M.; Barata, Carlos

Environmental factors affecting aquatic invertebrate communities were assessed using Daphnia magna in situ bioassays and biological indices based on community assemblages of benthic macroinvertebrates. Investigations were carried out in two heavily industrialized and urbanized river basins from the NE of Spain (Llobregat and Besos). Measures of energy consumption (i.e. algal grazing), and of specific biochemica...


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