17 documents found, page 1 of 2
A dual-function SNF2 protein drives chromatid resolution and nascent transcript...
Carmo, Catarina; Coelho, João; Silva, Rui D.; Tavares, Alexandra; Boavida, Ana; Gaetani, Paola; Guilgur, Leonardo G.; Martinho, Rui GonçaloMitotic chromatin is largely assumed incompatible with transcription due to changes in the transcription machinery and chromosome architecture. However, the mechanisms of mitotic transcriptional inactivation and their interplay with chromosome assembly remain largely unknown. By monitoring ongoing transcription in Drosophila early embryos, we reveal that eviction of nascent mRNAs from mitotic chromatin occurs a...
Endoplasmic reticulum membranes are continuously required to maintain mitotic s...
Araújo, Margarida; Tavares, Alexandra; Vieira, Diana V.; Telley, Ivo A.; Oliveira, Raquel A.Membrane organelle function, localization, and proper partitioning upon cell division depend on interactions with the cytoskeleton. Whether membrane organelles also impact the function of cytoskeletal elements remains less clear. Here, we show that acute disruption of the ER around spindle poles affects mitotic spindle size and function in Drosophila syncytial embryos. Acute ER disruption was achieved through t...
A dual‐function SNF2 protein drives chromatid resolution and nascent transcript...
Carmo, Catarina; Coelho, João; Silva, Rui; Tavares, Alexandra; Boavida, Ana; Gaetani, Paola; Guilgur, Leonardo G; Martinho, Rui GoncaloMitotic chromatin is largely assumed incompatible with transcription due to changes in the transcription machinery and chromosome architecture. However, the mechanisms of mitotic transcriptional inactivation and their interplay with chromosome assembly remain largely unknown. By monitoring ongoing transcription in Drosophila early embryos, we reveal that eviction of nascent mRNAs from mitotic chromatin occurs a...
Estratégias para um sector da cultura mais inclusivo: white paper e recomendações
Tavares, Alexandra; Vasconcelos, Beatriz; Trigo, Bruno; Pires, Cristiana Vale; Bossuet, Filipa; Baker, Julia; Silva, Mariana DuarteO MIL, nos formatos de festival e de convenção, antecipa futuras tendências e provoca as questões que determinam o futuro dos setores da música e da cultura.Na edição de 2021, o MIL introduziu um novo formato ao seu programa. Os laboratórios de ideias são sessões privadas de trabalho e de debate exploratório focadas em desafios atuais para o setor da música e da cultura. Tendo por base uma questão de partida, o...
An SNF2 helicase-like protein links mitotic transcription termination to sister...
Carmo, Catarina; Coelho, João; Silva, Rui; Tavares, Alexandra; Boavida, Ana; Gaetani, Paola; Martinho, Rui Gonçalo; Oliveira, Raquel A.Mitotic chromatin is largely assumed incompatible with transcription due to changes in the transcription machinery and chromosome architecture. However, the mechanisms of mitotic transcriptional inactivation and their interplay with chromosome assembly remain largely unknown. By monitoring ongoing transcription in Drosophila early embryos, we reveal that eviction of nascent mRNAs from mitotic chromatin occurs a...
Biallelic BUB1 mutations cause microcephaly, developmental delay, and variable ...
Carvalhal, Sara; Bader, Ingrid; Rooimans, Martin A.; Oostra, Anneke B.; Balk, Jesper A.; Feichtinger, René G.; Beichler, Christine; Speicher, Michael R.Budding uninhibited by benzimidazoles (BUB1) contributes to multiple mitotic processes. Here, we describe the first two patients with biallelic BUB1 germline mutations, who both display microcephaly, intellectual disability, and several patient-specific features. The identified mutations cause variable degrees of reduced total protein level and kinase activity, leading to distinct mitotic defects. Both patients...
Characterization of a MOB1 homolog in the Apicomplexan parasite toxoplasma gondii
Delgado, Inês L. S.; Tavares, Alexandra; Francisco, Samuel; Santos, Dulce; Coelho, João; Basto, Afonso P.; Zúquete, Sara; Müller, JoachimMonopolar spindle One Binder1 (MOB1) proteins are conserved components of the tumor-suppressing Hippo pathway, regulating cellular processes such as cytokinesis. Apicomplexan parasites present a life cycle that relies on the parasites’ ability to differentiate between stages and regulate their proliferation; thus, Hippo signaling pathways could play an important role in the regulation of the apicomplexan life c...
Metaphase chromosome structure is dynamically maintained by condensin I-directe...
Piskadlo, Ewa; Tavares, Alexandra; Oliveira, Raquel AMitotic chromosome assembly remains a big mystery in biology. Condensin complexes are pivotal for chromosome architecture yet how they shape mitotic chromatin remains unknown. Using acute inactivation approaches and live-cell imaging in Drosophila embryos, we dissect the role of condensin I in the maintenance of mitotic chromosome structure with unprecedented temporal resolution. Removal of condensin I from pre...
MOB1A
Carmona, Bruno; Tavares, Alexandra; Nolasco, Sofia; Leitão, Alexandre; Soares, HelenaThe accuracy of cell division is fundamental for the maintenance of cell ploidy and genomic stability. During cell division, many events, like DNA replication, chromosome segregation, mitosis completion, and cytokinesis, must be tightly controlled. The deregulation of these events is closely associated with severe pathology. Among other factors, the accuracy of cell division relies on the correct placement of t...
Mob1, hippo pathway member, is critical for toxoplasma gondii replication
Tavares, Alexandra; Delgado, Inês; Francisco, Samuel; Coelho, João; Leitão, Alexandre; Soares, Helena; Nolasco, SofiaToxoplasma gondii is an obligate intracellular parasite of great veterinary and medical importance. It is able to evade the immune system of the host by converting from rapidly proliferating tachyzoites to latent bradyzoite cysts and this parasite number control is a key to the success of the infection. Pathways controlling cell division/proliferation like the Hippo pathway are likely candidates for regulating ...