114 documents found, page 1 of 12
One-step purification of L-asparaginase from cell extracts using carbon xerogels
Cristóvão, Raquel O.; Barros, Rita A. M.; Marramaque, Teresa P.; Aguiar, Gonçalo G.; Almeida, Mafalda R.; Carabineiro, Sónia A. C.L-asparaginase (ASNase, EC 3.5.1.1) is an enzyme with wide applications in the pharmaceutical sector and food processing industries. It is mainly used as a biotherapeutic for treating Acute Lymphoblastic Leukemia (ALL) and to reduce acrylamide formation in starchy compounds. Despite its relevance, current purification methods for microbial enzymes involve complex and expensive techniques. To overcome this drawb...
Effective separation of nucleic acids from complex bacterial lysates using thre...
Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, FaniNo abstract available.
Irreversible temperature indicator based cellulose membranes conjugated with le...
Carvalho, Margarida; Tavares, Ana P. M.; Marques. Maria A.; Alves, Joaquim J.; Figueira, Joana; Morais, Maria; Pinto, Joana V.; Moreira, Felismina T. C.This research focuses on the development of thermochromic membranes made of cellulose acetate (CA) for temperature monitoring of sensitive food products. Two dual TC membranes developed for the control of different temperature ranges were formulated using a three-component system: a leuco-dye membrane (crystal violet lactone, CVL) integrated with an acidic membrane containing the color developer (salicylic acid...
Irreversible temperature indicator based cellulose membranes conjugated with le...
Carvalho, Margarida; Tavares, Ana P. M.; Marques, Maria A.; Alves, Joaquim J.; Figueira, Joana; Morais, Maria; Pinto, Joana V.; Moreira, Felismina T. C.This research focuses on the development of thermochromic membranes made of cellulose acetate (CA) for temperature monitoring of sensitive food products. Two dual TC membranes developed for the control of different temperature ranges were formulated using a three-component system: a leuco-dye membrane (crystal violet lactone, CVL) integrated with an acidic membrane containing the color developer (salicylic acid...
Supported ionic liquids to purify microbial L-Asparaginase
Almeida, Mafalda R.; Nunes, João C. F.; Pereira, Matheus M.; Bento, Heitor B. S.; Pedrolli, Danielle B.; Santos-Ebinuma, Valéria C.; Neves, Márcia C.L-Asparaginase (ASNase) is a versatile enzyme that converts L-asparagine into ammonia and aspartic acid. This enzyme has applications in the food industry and health sector. However, high purity ASNase is required, resulting in high production costs. Therefore, in this work, two supported ionic liquids (SILs), specifically silica modified with dimethylbutylpropylammonium chloride ([Si][N3114]Cl) or triethylprop...
Investigation of laccase activity in cholinium-based ionic liquids using experi...
Chan, Kam Khong; Pereira, Ana F.; Valente, Ana I.; Tavares, Ana P. M.; Coutinho, João A. P.; Ooi, Chien WeiLaccases hold great potential for biotechnological applications, particularly in environmental pollutant remediation. Laccase activity is governed by the solvent environment, and ionic liquids (ILs) emerge as a versatile solvent for activation or stabilization of enzymes. Herein, effects of cholinium-based ILs formulated with carboxylic acids, inorganic acid, and amino acids as anionic species, on the catalytic...
Portable colorimetric sensors for L-asparagine detection
Nunes, João C. F.; Neves, Márcia C.; Freire, Mara G.; Tavares, Ana P. M.L-asparaginase (ASNase) is an amidohydrolase enzyme used in the pharmaceutical industry as an anticancer agent in lymphoproliferative disorders treatment. ASNase is also applied in biosensors for L-asparagine detection in blood serum samples of acute lymphoblastic leukemia (ALL) patients. Nevertheless, the high cost of non-portable and sensitive L-asparagine detection methods, mainly performed by chromatography...
Recombinant laccase biosynthesis for efficient polydopamine coating
Magalhães, Flávia F.; Neves, Márcia C.; Pedro, Augusto Q.; Freire, Mara G.; Santos-Ebinuma, Valéria C.; Tavares, Ana P. M.Laccases are versatile biocatalysts with interest for various industrial applications. This study reports the expression of Trametes versicolor laccase in Komagataella phaffii X33. The cultivation parameters (methanol and CuSO4 concentration, and temperature) for recombinant laccase production were studied in an orbital shaker. Enhanced laccase production was achieved by adding 1 % (v/v) methanol daily, supplem...
Recent developments and challenges in the application of fungal laccase for the...
Magalhães, Flávia F.; Pereira, Ana F. S.; Cristóvão, Raquel O.; Barros, Rita A. M.; Faria, Joaquim L.; Silva, Cláudia G.; Freire, Mara G.According to the European Environment Agency, the textile industry is responsible for 20% of global water pollution due to dyeing and finishing products, thus facing severe environmental challenges. It is essential to design more biocompatible and sustainable treatment processes capable of removing dyes from industrial wastewater to fight this environmental hazard. Chemical industries must change traditional ch...
Enhancing plastic waste recycling: evaluating the impact of additives on the en...
Aguiar, Mariana I. S.; Sousa, Andreia F.; Teixeira, Gabriel; Tavares, Ana P. M.; Ferreira, Ana M.; Coutinho, João A. P.While (bio)degradation of polymers is a recognized challenge, the influence of additives on this process remains poorly understood. Their presence in commercial polyethylene (PE) may inhibit the degradation process or complicate the recycling. This study aims to develop an enzymatic degradation process for post-consumer high-density polyethylene (HDPE). The HDPE degradation was performed using laccase from Tram...