8 documents found, page 1 of 1
Enhancing plastic waste recycling: Evaluating the impact of additives on the en...
Aguiar, Mariana I.S.; Sousa, Andreia F.; Teixeira, Gabriel; Tavares, Ana P.M.; Ferreira, Ana M.; Coutinho, João A. P.While (bio)degradation of polymers is a recognized challenge, the influence of additives on this process remains poorly understood. Their presence in commercial polyethylene (PE) may inhibit the degradation process or complicate the recycling. This study aims to develop an enzymatic degradation process for post-consumer high-density polyethylene (HDPE). The HDPE degradation was performed using laccase from Tram...
Supported ionic liquids as customizable materials to purify immunoglobulin G
Capela, Emanuel V.; Bairos, Jéssica; Pedro, Augusto Q.; Neves, Márcia C.; Aires-Barros, M. Raquel; Azevedo, Ana M.; Coutinho, João A.P.Over the past few years, antibodies such as immunoglobulin G, IgG, have increased their market share as alternative therapeutics. However, their production at high purity levels is still costly due to the absence of a cost-effective platform for their recovery and purification from the complex biological media in which they are produced. This work describes, for the first time, that materials modified with ioni...
Multi-walled carbon nanotubes as a platform for Immunoglobulin G attachment
Almeida, Mafalda R.; Barros, Rita A.M.; Pereira, Matheus M.; Castro, Daniel; Faria, Joaquim L.; Freire, Mara G.; Silva, Cláudia G.; Tavares, Ana P.M.Nanomaterials have been extensively used in different applications due to their peculiar characteristics and nanoscale dimensions. Among nanoparticles, carbon-based nanomaterials are becoming highly attractive for biomedical applications such as diagnosis, tissue engineering, drug delivery, and biosensing. The conjugation of carbon-based nanomaterials with antibodies combines the properties of these materials w...
Biomedical-related applications of functionalized nanomaterials
Almeida, Mafalda R.; Neves, Márcia C.; Morales-Torres, Sergio; Freire, Mara G.; Faria, Joaquim L.; Santos-Ebinuma, Valéria C. [UNESP]; Silva, Cláudia G.Made available in DSpace on 2022-04-28T19:50:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01; This chapter provides an overview of the use of nanoparticles in the biomedical field. Due to their unique properties, nanomaterials have enabled the development of innovative technologies within the biomedical field, making them a popular topic of research. Nanoparticles (NPs) can be prepared from a wi...
A passive direct methanol fuel cell as transducer of an electrochemical sensor,...
Carneiro, Liliana P.T.; Ferreira, Nádia S.; Tavares, Ana P.M.; Pinto, Alexandra M.F.R.; Mendes, Adélio; Sales, Maria Goreti FerreiraThis work describes an electrochemical sensor with a biomimetic plastic antibody film for carcinoembryonic antigen (CEA, an important biomarker in colorectal cancer), integrated in the electrical circuit of a direct methanol fuel cell (DMFC), working in passive mode and used herein as power supply and signal transducer. In detail, the sensing layer for CEA consisted of a Fluorine-doped Tin Oxide (FTO) conductiv...
Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sul...
González Amado, Marlen; Tavares, Ana P.M.; Freire, Mara G.; Soto, Ana; Rodríguez, OscarCheese whey is an environmental problem as an effluent, but also a source of valuable raw materials, namely proteins and lactose. In this work, Aqueous Two-Phase Systems (ATPS) were studied for the recovery of lactose, BSA, β-lactoglobulin and α-lactalbumin, key components of cheese whey. ATPS formed by PEG (molecular weights: 200–8000 g.mol−1) with sodium or ammonium sulfate were investigated. Partitioning of ...
Insights on the laccase extraction and activity in ionic-liquid-based aqueous b...
Capela, Emanuel V.; Valente, Ana I.; Nunes, João C.F.; Magalhães, Flávia F.; Rodriguez, Oscar; Soto, Ana; Freire, Mara G.; Tavares, Ana P.M.Due to their catalytic properties, selectivity, and efficiency, enzymes are excellent biocatalysts. In particular, laccases are versatile multi-copper oxidases with great interest for a wide plethora of biotechnological and environmental applications. Even though several laccase-catalysed processes have been reported at an industrial level, the high costs of their downstream processing required to provide bioca...
Development of suported ionic liquids for the purification of antileukemic drugs
Almeida, Mafalda R.; Neves, Márcia C.; Santos-Ebinuma, Valéria C.; Freire, Mara G.; Tavares, Ana P.M.L-asparaginase (LA) is an antileukemic biopharmaceutical of current high-cost. LA is produced via fermentation and its purification usually comprises precipitation, liquid- liquid extraction and chromatography techniques [1]. This work aims to develop sustainable technologies to purify LA. Functionalized nanomaterials, namely supported ionic liquids (SILs), are used as cost-effective purification techniques for...