8 documents found, page 1 of 1
Strychnos pseudoquina and its purified compounds present an effective in vitro ...
Lage, Paula Sousa; Andrade, Pedro Henrique Rocha de; Lopes, Amanda de Santana; Fumagalli, Miguel Angel Chávez; Valadares, Diogo GarciaThe development of new and cost-effective alternative therapeutic strategies to treat leishmaniasis has become a high priority. In the present study, the antileishmanial activity of Strychnos pseudoquina St. Hil. was investigated and pure compounds that presented this biological effect were isolated. An ethyl acetate extract was prepared, and it proved to be effective against Leishmania amazonensis. A bioactivi...
Poloxamer 407 (Pluronic® F127)-based polymeric micelles for amphotericin B : in...
Mendonça, Débora Vasconcelos Costa; Lage, Letícia Martins dos Reis; Lage, Daniela Pagliara; Chávez Fumagalli, Miguel Angel; Ludolf, FernandaIn the present study, a Poloxamer 407-based amphotericin B (AmpB)-containing polymeric micelles system (AmpB/M) was employed in the treatment of Leishmania amazonensis-infected BALB/c mice. Initially, the in vitro antileishmanial activity (IC50 value) of AmpB/M and B-AmpB/M (empty micelles) against stationary promastigotes and amastigotes-like forms of the parasites was determined, and results were of 1.83 ± 0....
Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs u...
Chávez Fumagalli, Miguel Angel; Martins, Vívian Tamietti; Testasicca, Miriam Conceição de Souza; Lage, Daniela Pagliara; Costa, Lourena EmanueleIn Brazil, the percentage of infected dogs living in areas where canine visceral leishmaniasis (CVL) is endemic ranges from 10 to 62%; however, the prevalence of infection in dogs is probably higher than figures reported from serological studies. In addition, problems with the occurrence of false-positive or false-negative results in the serodiagnosis of CVL have been reported. The present work analyzed the pot...
Treatment of murine visceral leishmaniasis using an 8-hydroxyquinoline-containi...
Duarte, Mariana Costa; Lage, Letícia Martins dos Reis; Lage, Daniela Pagliara; Martins, Vivian Tamietti; Carvalho, Ana Maria Ravena SeverinoNewtherapeutics are urgently needed to treat visceral leishmaniasis (VL). Due to the fact that drug discovery is a long and expensive process, the development of delivery systems to carry old and toxic drugs could be considered, as well as the evaluation of new molecules that have already shown to present biological activity. In this context, the present study evaluated the in vitro and in vivo antileishmanial ...
Evaluation of immune responses and protection induced by A2 and nucleoside hydr...
Zanin, Francisca Helena Calheiros; Coelho, Eduardo Antônio Ferraz; Tavares, Carlos Alberto Pereira; Silva, Eduardo de Almeida Marques daSeveral antigens have been tested as vaccine candidates against Leishmania infections but controversial results have been reported when different antigens are co-administered in combined vaccination protocols. Immunization with A2 or nucleoside hydrolase (NH) antigens was previously shown to induce Th1 immune responses and protection in BALB/c mice against Leishmania donovani and L. amazonensis (A2) or L. donov...
Comparative evaluation of phenol and thimerosal as preservatives for a candidat...
Mayrink, Wilson; Tavares, Carlos Alberto Pereira; Deus, Rosângela Barbosa de; Pinheiro, Melina Barros; Guimarães, Tânia Mara Pinto DabésFor decades thimerosal has been used as a preservative in the candidate vaccine for cutaneous leishmaniasis, which was developed by Mayrink et al. The use of thimerosal in humans has been banned due to its mercury content. This study addresses the standardization of phenol as a new candidate vaccine preservative. We have found that the proteolytic activity was abolished when the test was conducted using the can...
Synergistic action of praziquantel and host specific immune response against Sc...
Ribeiro, Fabio; Mello, Rômulo Teixeira de; Tavares, Carlos Alberto Pereira; Kusel, John Robert; Coelho, Paulo Marcos ZechA interação entre a resposta imune específica ao Schistosoma mansoni e praziquantel (PZQ) foi avaliada em modelo murino. Em camundongos portadores de imunidade concomitante, parasitos com 6 dias de idade e tratados com PZQ, foram eliminados mais eficazmente do que parasitos de apenas 24 h, apesar de ambos mostrarem uma redução significativa da carga parasitária quando comparados com os respectivos controles tra...
Schistosoma mansoni: protective immunity in mice cured by chemotherapy at the c...
Bezerra, Fernando Schemelzer de Moraes; Coelho, Paulo Marcos Zech; Tavares, Carlos Alberto PereiraAiming at demonstrating a decrease of acquired immunity after chemotherapeutic cure, a group of mice was infected with 25 Schistosoma mansoni cercariae (LE strain). A part of these animals was treated with 400 mg/kg oxamniquine, at 120 days after infection. Challenge infections were carried out at 45, 90 and 170-day-intervals after treatment (185, 210 and 290 days after primoinfection, respectively). Recovery o...